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    Wyświetlanie 1-9 z 9
    Tytuł:
    The occurrence of Toxoplasma gondii and Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from East Poland with the use of PCR
    Autorzy:
    Sroka, J
    Szymanska, J.
    Wojcik-Fatla, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/51137.pdf
    Data publikacji:
    2009
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Polska
    tick
    Ixodes ricinus
    Toxoplasma gondii
    Borrelia burgdorferi
    occurrence
    polymerase chain reaction
    genotyping
    B1 gene
    human population
    infection
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2009, 16, 2; 313-319
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Ocena wspolwystepowania Toxaplasma gondii i Borrelia burgdorferi w kleszczach Ixodes ricinus w oparciu o badania PCR
    Autorzy:
    Wojcik-Fatla, A.
    Sroka, J.
    Zajac, V.
    Cisiak, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/839306.pdf
    Data publikacji:
    2010
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    pasozyty
    Toxoplasma gondii
    Borrelia burgdorferi
    wspolwystepowanie
    kleszcze
    Ixodes ricinus
    metody badan
    lancuchowa reakcja polimerazy
    Źródło:
    Annals of Parasitology; 2010, 56, 3; 207
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Optimization of flotation, DNA extraction and PCR methods for detection of Toxoplasma gondii oocysts in cat faeces
    Autorzy:
    Sroka, J.
    Karamon, J.
    Dutkiewicz, J.
    Wójcik-Fatla, A.
    Cencek, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/2081971.pdf
    Data publikacji:
    2018
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    flotation
    Toxoplasma gondii
    faeces
    Real time PCR
    nested PCR
    cats
    oocysts detection
    Opis:
    Introduction and objective. The aim of the study was to compare the effectiveness of selected oocysts concentration methods, DNA extraction protocols and PCR assays targeting the B1 gene, for the development of procedures which would be effective and useful in laboratory practice for the detection of T. gondii in faecal samples from cats. Materials and method. In order to compare the influence of the flotation fluids on microscopy and PCR detection of T. gondii, saturated solutions of saccharose, MgSO4, ZnSO4 and NaNO3 were used. To determine the sensitivity of PCR tests used: Real time PCR (RT) and nested PCR, water samples spiked with T. gondii tachyzoites and oocysts were tested. DNA was extracted using DNeasy Blood and Tissue Kit (Qiagen) (K1). The same PCR tests were used to assess the efficacy of T. gondii DNA detection in samples of cat faeces spiked with oocysts, using DNA extraction by a K1 set and a K2 set (QIamp DNA Stool Mini Kit, (Qiagen). Results. The initial results showed that NaNO3 was most useful as a flotation fluid due to the lack negative effect on the oocysts and amplification efficacy in PCR. The level of detection for water samples (100 μl) was determined as 100 tachyzoites and 1–50 oocysts in RT, and 2–20 oocysts in nested PCR. The limit of detection (LD) for stool samples (250 mg) spiked with oocysts, where the K1 set was used, determined as 250 and 5 oocysts in RT and nested PCR, respectively. For samples extracted with the K2 set, LD in RT was determined as 1–50 oocysts (depending on the variant) and 50 oocysts in nested PCR. Conclusions. The most effective methods for detection of T. gondii in cat faeces seem to be centrifugal flotation with NaNO3, followed by DNA extraction with removing of inhibitors (K2 set) and Real Time PCR targeting B1 gene.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2018, 25, 4; 680-685
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The occurrence of Toxoplasma gondii infection in people and animals from rural environment of Lublin region - estimate of potential role of water as a source of infection
    Autorzy:
    Sroka, J
    Wojcik-Fatla, A.
    Szymanska, J.
    Dutkiewicz, J.
    Zajac, V.
    Zwolinski, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/49789.pdf
    Data publikacji:
    2010
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    parasite
    protozoan parasite
    Toxoplasma gondii
    occurrence
    people
    animal
    domestic animal
    rural environment
    Lublin region
    epidemiology
    serological examination
    farm inhabitant
    potable water
    polymerase chain reaction
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2010, 17, 1; 125-132
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Prevalence of Toxoplasma gondii infection in cats in southwestern Poland
    Autorzy:
    Sroka, J.
    Karamon, J.
    Dutkiewicz, J.
    Wójcik-Fatla, A.
    Zając, V.
    Cencek, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/2081746.pdf
    Data publikacji:
    2018
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Polska
    PCR
    seroprevalence
    Toxoplasma gondii
    IFAT
    cats
    oocysts
    coproscopy
    Opis:
    Objective. An assessment of the prevalence of Toxoplasma gondii infection in cats from southwestern Poland using serology, coproscopy and PCR methods. Materials and method. In total, 208 cats (139 females and 68 males), aged 0.5–12 years (mean=2.6) from 25 localities in southwestern Poland were examined by indirect immunofluorescence assay (IFAT) to estimate the T. gondii serological status. Faecal samples of 41 cats were examined for the presence of oocysts/DNA T. gondii by microscopy and Real-time/nested PCR. After flotation (with NaNO3), pellets from faecal samples were disrupted by 10 cycles of freezing (liquid nitrogen) and warming. DNA was extracted using QIamp DNA Stool Mini Kit (Qiagen), according to the manufacturer’s instruction. Results. The positive results in IFAT for anti-T. gondii IgG and IgM antibodies were found in 143 of 208 tested cats (68.8%). Among positive results, 14.5%, 34.1% and 51.4% were detected in titre ranges 128–512, 1,000–2,000 and ≥ 4,000, respectively. In 23.1% of cat sera anti- T. gondii IgM antibodies were found. The prevalence of anti-Toxoplasma antibodies was significantly greater in older cats (>1 year) (83.5%) than in younger cats (48.3%) (P<0.05), in females (74.1%) than in males (58.8%) (P<0.05), and in cats kept outdoors than indoors (69.7% vs. 16.7%) (P<0.01). Among the 41 faecal samples examined, the presence of structures resembling T. gondii oocysts was found in 2 samples (4.9%), and for one of these samples (2.4% of the total) the result was also confirmed by PCR. Conclusions. T. gondii infection in domestic cats is highly prevalent in southwestern Poland. Information on the prevalence of infection in cats can be useful for assessing T. gondii environmental contamination and the risk for public health.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2018, 25, 3; 1-5
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Preliminary study on the occurrence of Toxoplasma gondii in Ixodes ricinus ticks from north-western Poland with the use of PCR
    Autorzy:
    Sroka, J
    Wojcik-Fatla, A.
    Zwolinski, J.
    Zajac, V.
    Sawczuk, M.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/51615.pdf
    Data publikacji:
    2008
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    polymerase chain reaction
    parasite
    genotyping
    Polska
    tick
    toxoplasmosis
    Ixodes ricinus
    B1 gene
    zoonotic disease
    occurrence
    Toxoplasma gondii
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2008, 15, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Toxoplasma gondii infection in selected species of free-living animals in Poland
    Autorzy:
    Sroka, J.
    Karamon, J.
    Wójcik-Fatla, A.
    Dutkiewicz, J.
    Bilska-Zając, E.
    Zając, V.
    Piotrowska, W.
    Cencek, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/2085130.pdf
    Data publikacji:
    2019
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Polska
    Toxoplasma gondii
    nested PCR
    genotyping
    free-living animals
    Opis:
    Introduction and objective. Free-living animals can play an important role as a reservoir of Toxoplasma gondi;, however, data concerning this issue in Poland are still limited.The aim of study was to assess the occurrence of T. gondii infection by using molecular methods in free-living animals in selected regions of Poland. Materials and method. Tissues samples of 396 animals (foxes, muskrats, birds, martens, badgers, polecats, raccoons, minks, raccoon dogs, otters, small rodents and insectivores, and grass snakes were collected from various regions of Poland. After samples digestion, DNA was isolated using QIAmp DNA Mini Kit (Qiagen). DNA extraction from small rodents and insectivores samples was performed without digestion. Next, nested PCR (B1 gene) and, for a part of nested PCR positive amplicons, RFLP PCR, were performed according to the method by Grigg and Boothroyd (2001). The other part of nested PCR positive DNA isolates were genotyped using 5 genetic markers: SAG1, SAG2 (5’- and 3’), SAG3, BTUB and GRA6, based on the method by Dubey et al. (2006). These PCR products were sequenced and compared with the NCBI database using Blast. Results. In total, in 50 of the 396 examined animals DNA of T. gondii was detected (12.6%). The highest percentages of positive results in PCR was obtained in martens (40.9%) and badgers (38.5%), lower in birds (27.3%) and the lowest in foxes (7.4%). The RFLP and multilocus PCR analysis showed the dominance of T. gondii clonal type II (or II/III). Conclusions. The results of this study indicate the frequent T. gondii infection among free-living animals in Poland, especially martens and badgers, which may indirectly indicate that these animals contribute to the spread of the parasite in the sylvatic environment in Poland. The genotyping analysis showed the dominance of T. gondii clonal type II (or II/III).
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2019, 26, 4; 656-660
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Study on prevalence of Toxoplasma gondii infection in free-living animals in Poland
    Autorzy:
    Sroka, J.
    Karamon, J.
    Bilska-Zajac, E.
    Stojecki, K.
    Rozycki, M.
    Kusyk, P.
    Wojcik-Fatla, A.
    Zajac, V.
    Cencek, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/6474.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    Toxoplasma gondii
    animal infection
    prevalence
    free living animal
    Polska
    Źródło:
    Annals of Parasitology; 2016, 62, Suppl.
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Potential sources of infection with selected zoonotic agents in the veterinary work environment - pilot studies
    Autorzy:
    Wójcik-Fatla, A.
    Sroka, J.
    Zając, V.
    Sawczyn-Domańska, A.
    Kloc, A.
    Zwoliński, J.
    Kłapeć, T.
    Studzińska, M.B.
    Chmura, R.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2085448.pdf
    Data publikacji:
    2020
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    environment
    Coxiella burnetii
    Toxoplasma gondii
    veterinarians
    Leptospira spp
    Cryptosporidium spp
    Giardia duodenalis
    Opis:
    Introduction and Objective. The problem of occupational biohazards is very important, especially in the field of agriculture and in human and veterinary medicine. The aim of the study was to determine the potential sources of infection in veterinary professionals with selected zoonotic agents, including: Toxoplasma gondii, Giardia duodenalis, Leptospira spp., Cryptosporidium spp. and Coxiella burnetii. Materials and Method. A total of 50 air samples from barns, piggeries and veterinary surgeries were examined for the presence of Leptospira spp. and C. burnetii DNA. Serum samples of 86 pigs and 80 cows were tested for the presence of antibodies to Leptospira spp. and to phase I and II C. burnetii antigens. Serum of 70 cats were tested for the presence of antibodies to T. gondii and 65 samples of cat faeces for the presence of T. gondii oocysts. The presence of G. duodenalis and Cryptosporidium spp. were examined in 50 of dog faeces and 50 of bovine faeces samples. Results. DNA of Leptospira spp. was detected in 2 air samples from the piggeries (4%). C. burnetii DNA was not found in any sample. Anti-Leptospira spp. antibodies were detected in 51 (59.3%) of examined pigs. Neither anti-Leptospira spp. nor anti-C. burnetii antibodies were found among samples of bovine serum. Anti-T. gondii antibodies was found in 52 cat serum samples (74.3%). Among samples of cat faeces, no T. gondii oocysts were detected. In one sample of cattle stool (2%), G. duodenalis was detected and in another (2%) – Cryptosporidium spp. G. duodenalis was detected in 7 samples (14%) and Cryptosporidium spp. in 2 samples (2%) of dog faeces. Conclusions. The results of this study demonstrate the potential risk of infection with Leptospira spp. in veterinarians working with pigs. Veterinarians could be also be at risk of infection with T. gondii and G. duodenalis.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2020, 27, 1; 146-150
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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