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Wyszukujesz frazę "Pseudomonas" wg kryterium: Temat


Tytuł:
Media optimization for depolymerization of alginate by Pseudomonas aeruginosa AG LSL-11
Autorzy:
Koti, B.A.
Manohar, S.
Lalitha, J.
Powiązania:
https://bibliotekanauki.pl/articles/11457.pdf
Data publikacji:
2014
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
depolymerization
alginate
Pseudomonas aeruginosa
bacteria
Opis:
An agar degrading bacterium Pseudomonas aeruginosa AG LSL-11 was acclimatized to alginate for the production of alginase. Production parameters such as pH, temperature, influence of simple carbohydrates and nitrogen sources, and effect of NaCl on growth and alginase production were carried out. Maximum growth was observed at pH 9.0 and 35 °C, while alginase was produced optimally at pH 9.0 and 30 °C. The alginase produced by Pseudomonas aeruginosa AG LSL-11 was inducible by alginate, and repressed by other simple sugar when supplemented along with alginate in the medium. The bacterium did not require NaCl for growth and production of alginase. The activity staining of partially purified culture supernatant after native PAGE revealed the presence of a single alginase.
Źródło:
International Letters of Natural Sciences; 2014, 14
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Methods for eradication of the biofilms formed by opportunistic pathogens using novel techniques – A review
Autorzy:
Zabielska, Julia
Tyfa, Agnieszka
Kunicka-Styczyńska, Alina
Powiązania:
https://bibliotekanauki.pl/articles/764814.pdf
Data publikacji:
2016
Wydawca:
Uniwersytet Łódzki. Wydawnictwo Uniwersytetu Łódzkiego
Tematy:
biofilm eradication
Pseudomonas aeruginosa
microbial colonization
Opis:
W niekorzystnych warunkach środowiska, mikroorganizmy zasiedlają zarówno powierzchnie abiotyczne, jak i biotyczne takie jak tkanki zwierzęce czy roślinne, tworząc struktury biofilmu charakteryzujące się wysoką opornością. Adhezja mikroorganizmów, szczególnie patogenów oportunistycznych, niesie niebezpieczeństwo zasiedlania materiałów medycznych, co może doprowadzić do infekcji u osób z obniżoną odpornością. Chociaż dotychczasowe badania wskazują różne metody zapobiegania tworzeniu biofilmu, jego całkowita eliminacja ze środowiska jest nadal niemożliwa. Przedstawione opracowanie stanowi przegląd nowoczesnych metod usuwania dojrzałego biofilmu tworzonego przez patogeny oportunistyczne. Spośród wielu metod opisano m.in. zastosowanie: zimnej plazmy, ultradźwięków, pola elektrycznego, ozonowania wody, terapii fagowej, enzymów działających bezpośrednio na macierz biofilmu, bakteriocyn, środków chemicznych syntetycznych oraz pochodzenia naturalnego.
The inconvenient environmental conditions force microorganisms to colonize either abiotic surfaces or animal and plant tissues and, therefore, form more resistant structures – biofilms. The phenomenon of microbial adherence, opportunistic pathogens in particular, is of a great concern. Colonization of medical devices and biofilm formation on their surface, may lead to severe infections mainly in humans with impaired immune system. Although, current research consider various methods for prevention of microbial biofilms formation, still, once a biofilm is formed, its elimination is almost impossible. This study focuses on the overview of novel methods applied for eradication of mature opportunistic pathogens' biofilms. Among various techniques the following: cold plasma, electric field, ultrasounds, ozonated water treatment, phagotherapy, matrix targeting enzymes, bacteriocins, synthetic chemicals and natural origin compounds used for biofilm matrix disruption were briefly described.
Źródło:
Acta Universitatis Lodziensis. Folia Biologica et Oecologica; 2016, 12; 26-37
1730-2366
2083-8484
Pojawia się w:
Acta Universitatis Lodziensis. Folia Biologica et Oecologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Increased production of carrageenase by Pseudomonas aeruginosa ZSL-2 using Taguchi experimental design
Autorzy:
Ziayoddin, M.
Lalitha, J.
Shinde, M.
Powiązania:
https://bibliotekanauki.pl/articles/11042.pdf
Data publikacji:
2014
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
production
carrageenase
Pseudomonas aeruginosa
Taguchi method
Opis:
The culture conditions for the production of carrageenase were optimized using one-factor-at-a-time method combined with orthogonal array design. With the one-factor-at-a-time method revealed optimal conditions for carrageenase production were 24 h of fermentation period, 28 °C incubation temperature at pH 8.0 with NaNO3 as nitrogen source and carrageenan as carbon source in MMS media. Further optimization of carrgeenase production by using orthogonal experimental design L9 (34) with four factors, temperature, pH, NH4NO3 and carrageenan with their relevant levels revealed optimised conditions for carrageenase production were temperature of 28 °C, pH 8.0, 2 g L-1 NaNO3 and 2 g L-1 carrageenan. The order of the factors affecting the fermentation process was found to be temperature > pH > NaNO3 > carrageenan. The temperature played a significant role on the carrageenase production. Higher carrageenase yield with activity of 0.542 ±0.045 U ml-1 was obtained in the optimised medium when compared to those of basal medium. Carrageenase hydrolysed products of carrageenan were identified by LC-ESI-MS as neocarrabiose, neocarrabiose-4 sulfate, neocarratetraose, neocarratetraose-4 sulfate, anhydrogalactose, galactose, galactose-4 sulphate and sulphate.
Źródło:
International Letters of Natural Sciences; 2014, 12, 2
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A Bioremediation Study of Raw and Treated Crude Petroleum Oil Polluted Soil with Aspergillus niger and Pseudomonas aeruginosa
Autorzy:
Ojewumi, M. E.
Anenih, E. V.
Taiwo, O. S.
Adekeye, B. T.
Awolu, O. O.
Ojewumi, E. O.
Powiązania:
https://bibliotekanauki.pl/articles/124955.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
bioremediation
Aspergillus niger
Pseudomonas aeruginosa
pollution
Opis:
This study was conducted to investigate the degree of bioremediation that would occur in the samples of soil polluted with raw and treated crude petroleum (oil) with the aid of Aspergillus niger (fungi) and Pseudomonas aeruginosa (bacteria). This was achieved by monitoring the Organic carbon content in the soil over 45 days and pH over 25 days. Four systems of 500 g soil were polluted with 40 g treated crude petroleum, while four systems were contaminated with 40 g raw crude petroleum. The eight systems were labeled accordingly. Two systems for raw crude control and treated crude control (RCC and TCC) were left as control, two systems for raw crude Aspergillus niger and treated crude Aspergillus niger (RCA and TCA) were treated with Aspergillus niger only, two systems for raw crude Pseudomonas aeruginosa and treated crude Pseudomonas aeruginosa (RCP and TCP) were treated with Pseudomonas aeruginosa only and the last two systems for raw crude Aspergillus niger and Pseudomonas aeruginosa (RCAP and TCAP) were treated with both Pseudomonas aeruginosa and Aspergillus niger. At the end of the bioremediation period, the results obtained showed that the pH was not particularly a solid parameter to estimate the degree of bioremediation. This is because a proper trend in the results obtained could not be determined. Additionally, it was observed that Aspergillus niger (fungi) and Pseudomonas aeruginosa (bacteria) alone and separate remediate raw crude polluted soil better than treated crude polluted soil. This was determined by the TOC (Total Organic Carbon) values on the 45th day of the experiment.
Źródło:
Journal of Ecological Engineering; 2018, 19, 2; 226-235
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Opportunistic Gram-negative rods capability of creating biofilm structures on polivynyl chloride and styrene-acronitrile copolymer surfaces
Autorzy:
Zabielska, Julia
Kunicka-Styczyńska, Alina
Rajkowska, Katarzyna
Tyfa, Agnieszka
Powiązania:
https://bibliotekanauki.pl/articles/1038901.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Pseudomonas aeruginosa
biofilm
environmental strains
polymer surfaces
Opis:
Biofilms are highly organized microbial communities displaying high resistance to disinfectants and other external environmental factors. Medical equipment, such as stents and catheters, can be colonized by a variety of bacteria including opportunistic pathogens circulating in the environment and dangerous to immunocompromised patients. Application of materials resistant to biofilm formation will minimize the risk of patients' infection. Hence, the aim of this research was to determine the biofilm growth of environmental bacteria isolates on polyvinyl chloride and styrene-acronitrile copolymer surfaces. Nine strains (Pseudomonas aeruginosa, Burkholderia cepacia and Serratia liquefacies) isolated from cosmetics, and a reference P. aeruginosa strain ATCC 15442, were tested. The ability and dynamics of biofilm formation on intubation catheters (30°C, up to 24 h) in bacterial growth cultures (107-108 CFU/ml) was investigated, with subsequent sonication and quantification by agar plate count method. The results indicated that all the tested bacteria expressed a strong ability for the polymer surface adhesion, reaching 4.6 to 6.7 log CFU/cm2 after 30 minutes. Moreover, for the majority of strains, the level of 24-hour biofilm production was from 6.67-7.61 log CFU/cm2. This research indicates that the environmental strains circulating between the cosmetics and patients may pose a threat of biofilm formation on medical equipment surfaces, and presumably in the clinical surroundings as well.
Źródło:
Acta Biochimica Polonica; 2015, 62, 4; 733-737
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Impedance Sensors Made in PCB and LTCC Technologies for Monitoring Growth and Degradation of Pseudomonal Biofilm
Autorzy:
Chabowski, K.
Junka, A. F.
Piasecki, T.
Nowak, D.
Nitsch, K.
Smutnicka, D.
Bartoszewicz, M.
Moczała, M.
Szymczyk, P.
Powiązania:
https://bibliotekanauki.pl/articles/221326.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Pseudomonas aeruginosa
biofilm
interdigitated sensor
impedance spectroscopy
Opis:
The suitability of low-cost impedance sensors for microbiological purposes and biofilm growth monitoring was evaluated. The sensors with interdigitated electrodes were fabricated in PCB and LTCC technologies. The electrodes were golden (LTCC) or gold-plated (PCB) to provide surface stability. The sensors were used for monitoring growth and degradation of the reference ATCC 15442 Pseudomonas aeruginosa strain biofilm in invitro setting. During the experiment, the impedance spectra of the sensors were measured and analysed using electrical equivalent circuit (EEC) modelling. Additionally, the process of adhesion and growth of bacteria on a sensor’s surface was assessed by means of the optical and SEM microscopy. EEC and SEM microscopic analysis revealed that the gold layer on copper electrodes was not tight, making the PCB sensors susceptible to corrosion while the LTCC sensors had good surface stability. It turned out that the LTCC sensors are suitable for monitoring pseudomonal biofilm and the PCB sensors are good detectors of ongoing stages of biofilm formation.
Źródło:
Metrology and Measurement Systems; 2017, 24, 2; 369-380
0860-8229
Pojawia się w:
Metrology and Measurement Systems
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Hemorrhagic pneumonia in neonatal minks in Greece concomitant with Leismania infantum detection
Autorzy:
Filioussis, G.
Petridou, E.
Papadopoulos, D.
Karavanis, E.
Morgan, E.
Billinis, C.
Papadopoulos, E.
Powiązania:
https://bibliotekanauki.pl/articles/2087727.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
mink
hemorrhagic pneumonia
Pseudomonas aeruginosa
Leismania infantum
Greece
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 2; 405-408
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cross-infections with Pseudomonas aeruginosa in patients with cystic fibrosis attending the Warsaw Centre
Zakażenia krzyżowe Pseudomonas aeruginosa u pacjentów z mukowiscydozą pozostających pod opieką Instytutu Matki i Dziecka
Autorzy:
Milczewska, Justyna
Wołkowicz, Tomasz
Zacharczuk, Katarzyna
Kwiatkowska, Monika
Sands, Dorota
Powiązania:
https://bibliotekanauki.pl/articles/1032970.pdf
Data publikacji:
2015
Wydawca:
Wydawnictwo ALUNA
Tematy:
PFGE
Pseudomonas aeruginosa
cross-infections
cystic fibrosis
Opis:
Aim: 1. To assess the prevalence of cross-infections with P. aeruginosa in order to evaluate the epidemiological situation of this infection in patients with cystic fibrosis attending our centre; 2. To correlate the clinical features of the patients carrying a potentially transmissible strain with the entire study group in order to determine the risk factors and possible effects of its acquisition. Material and methods: 170 Pseudomonas aeruginosa strains obtained from the respiratory tract of 75 cystic fibrosis patients attending the Warsaw Centre in 2011 and 2012 were typed using restriction enzyme analysis-pulsed field gel electrophoresis (Spe I restriction enzyme was used). Simultaneously, the information concerning contacts between patients, as well as several clinical data regarding the course of the disease were collected. Results: Twenty four clusters of strains were detected. The main cluster included 49 isolates derived from 21 patients. The other detected clusters included 2 to 12 isolates derived from 1 to 7 patients. Three clusters comprised the isolates derived from three pairs of siblings. There were 15 clusters containing 2 to 7 strains belonging to the same patient. The remaining 24 patients were infected with their own strains, not fitting any clonal group. Several clinical parameters showed that the 21 patients whose strains constituted the main cluster, were in worse clinical condition than the other patients in the study group. Moreover, the total duration of their hospitalizations in order to perform intravenous antibiotic treatment was longer. Conclusions: 1. Frequent hospitalizations of CF patients with a more severe course of the disease seem to be a risk factor of cross-infections with P. aeruginosa. 2. Intensification of measures to prevent cross-infection, such as hygienic precautions, patient segregation, introduction of home intravenous antibiotic therapy programme, as well as further education of patients and their parents should lead to the improvement of the epidemiological situation in our centre.
Cel: 1. Ocena częstości występowania zakażeń krzyżowych Pseudomonas aeruginosa i ich możliwych konsekwencji klinicznych u pacjentów chorujących na mukowiscydozę leczonych w naszym ośrodku; 2. Porównanie cech klinicznych grupy pacjentów, u których doszło do zakażenia krzyżowego z cechami klinicznymi całej grupy badanej, w celu określenia czynników ryzyka i możliwych skutków takiego zakażenia. Materiał i metody: 170 szczepów Pseudomonas aeruginosa wyizolowanych w latach 2011-2012 z dróg oddechowych 75 pacjentów z mukowiscydozą pozostających pod opieką Instytutu Matki i Dziecka typowano metodą makrogenomowej analizy restrykcyjnej z elektroforezą w zmiennym polu elektrycznym (z wykorzystaniem endonukleazy Spe I). Zbierano wywiady dotyczące najważniejszych parametrów klinicznych, a także kontaktów pomiędzy pacjentami. Wyniki: W toku analizy wyodrębniono 24 grupy klonalne. Największa grupa zawierała 49 izolatów pochodzących od 21 chorych. Inne grupy obejmowały od 2 do 12 izolatów pochodzących od 1 do 7 pacjentów. Trzy grupy zawierały szczepy pochodzące od trzech rodzeństw. Piętnaście grup klonalnych obejmowało od 2 do 7 szczepów należących do tego samego pacjenta. Pozostałych 24 pacjentów było zakażonych szczepami, nie należącymi do żadnej grupy klonalnej. Ocena parametrów klinicznych wykazała, że przebieg choroby u 21 pacjentów, których szczepy stanowiły największą grupę klonalną, był cięższy niż u pozostałych pacjentów w grupie badanej. Także łączny czas trwania ich hospitalizacji (antybiotykoterapii dożylnych) był dłuższy. Wnioski: 1. Częste hospitalizacje pacjentów z mukowiscydozą w gorszym stanie klinicznym wydają się być istotnym czynnikiem ryzyka zakażeń krzyżowych Pseudomonas aeruginosa. 2. Intensyfikacja działań zapobiegających zakażeniom krzyżowym, takich jak środki higieny, izolacja chorych, wprowadzenie w Polsce programu domowej antybiotykoterapii dożylnej, a także dalsza edukacja pacjentów i ich rodziców odnośnie dróg transmisji zakażeń bakteryjnych, prawdopodobnie przyczynią się do poprawy sytuacji epidemiologicznej w naszym ośrodku.
Źródło:
Developmental Period Medicine; 2015, 1; 60-65
1428-345X
Pojawia się w:
Developmental Period Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evaluation of petroleum hydrocarbons adsorbtion and biodegradation by Pseudomonas aeruginosa cells entrapped into silica-alginate beads
Autorzy:
Mizielińska, Małgorzata
Kowalska, Urszula
Łopusiewicz, Łukasz
Powiązania:
https://bibliotekanauki.pl/articles/1179265.pdf
Data publikacji:
2017
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Pseudomonas aeruginosa
biodegradation
bioimmobilization
encapsulation
petroleum hydrocarbons
Opis:
The aim of the work was to elaborate new method of wool entrapping into silica alginate beads and checking of their adsorption properties. The purpose of the present study was also to determine water bioremediation efficiency using Pseudomonas aeruginosa cells immobilized into wool entrapped into silica-alginate beads. The results of study showed that obtained capsules are stable in saline water environment. Additionally neither silica adsorbers nor wool impacted negatively on viability of bacterial strains that were entrapped into these capsules. Moreover, it should be underline that 83,01% of adsorbed engine oil has been degraded by immobilized microorganisms after 3 months of biodegradation process. The results proved that properties and non-toxic character of these beads are suitable enough to use them in purification of water from petroleum hydrocarbons.
Źródło:
World Scientific News; 2017, 80; 256-267
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isolation and characterization of naphthalene biodegrading Methylobacterium radiotolerans bacterium from the eastern coastline of the Kingdom of Saudi Arabia
Autorzy:
Nzila, A.
Thukair, A.
Sankara, S.
Chanbasha, B.
Musa, M. M.
Powiązania:
https://bibliotekanauki.pl/articles/204862.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
biodegradation
bioremediation
naphthalene
Methylobacterium radiotolerans
Pseudomonas aeruginosa
Opis:
Bioremediation is based on microorganisms able to use pollutants either as a source of carbon or in co-metabolism, and is a promising strategy in cleaning the environment. Using soil contaminated with petroleum products from an industrial area in Saudi Arabia (Jubail), and after enrichment with the polycyclic aromatic hydrocarbon (PAH) naphthalene, a Methylobacterium radiotolerans strain (N7A0) was isolated that can grow in the presence of naphthalene as the sole source of carbon. M. radiotolerans is known to be resistant to gamma radiation, and this is the first documented report of a strain of this bacterium using a PAH as the sole source of carbon. The commonly reported Pseudomonas aeruginosa (strain N7B1) that biodegrades naphthalene was also identified, and gas chromatography analyses have shown that the biodegradation of naphthalene by M. radiotolerans and P. aeruginosa did follow both the salicylate and phthalate pathways.
Źródło:
Archives of Environmental Protection; 2016, 42, 3; 25-32
2083-4772
2083-4810
Pojawia się w:
Archives of Environmental Protection
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Outbreak of Pseudomonas aeruginosa mastitis in a dairy cow herd in northern Greece and its control with an autogenous vaccine
Autorzy:
Petridou, E.J.
Fragkou, I.A.
Lafi, S.Q.
Giadinis, N.D.
Powiązania:
https://bibliotekanauki.pl/articles/2087183.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
dairy cows
Pseudomonas aeruginosa
clinical mastitis
control
autogenous vaccine
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 2; 303-305
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Contact lens-related combined Pseudomonas aeruginosa, Acanthamoeba and Candida keratitis among adolescents girls
Autorzy:
Świerczyńska, Marta
Tronina, Agnieszka
Bogocz, Michał
Mrukwa-Kominek, Ewa
Filipek, Erita
Powiązania:
https://bibliotekanauki.pl/articles/2046699.pdf
Data publikacji:
2022-03-31
Wydawca:
Medical Education
Tematy:
infectious keratitis
contact lenses
Candida
Acanthamoeba
Pseudomonas aeruginosa
Opis:
Background: We present a case of combined Pseudomonas aeruginosa, Acanthamoeba and Candida keratitis and clinical course of treatment in two adolescents girls who did not follow the rules of proper use of soft contact lenses. Case reports: Two teenage contact lens users sought medical attention, reporting visual impairment, redness and pain of the eye. Slit lamp examination showed unilateral corneal ulcer. Confocal microscopy revealed images compatible with amoebic cysts and yeasts cells, whereas the results of conjunctival swabs confirmed the presence of Pseudomonas aeruginosa. After topical and general treatment in the first patient, the corneal defect was healed, and visual acuity improved. The girl remains under the constant supervision of an ophthalmological clinic. The medications were gradually reduced, but with time she developed crystalline keratopathy. Regrettably, the second patient, despite the applied treatment had recurrent inflammation and progressive corneal thinning, which required amniotic membrane transplantation to prevent perforation. Conclusion: The early identification of the pathogen as well as adequate treatment is crucial, as contact lens-associated keratitis are potentially sight threatening. Patient and caregiver education, adequate contact lens hygiene and consistent follow-up are important to prevent serious complications.
Źródło:
OphthaTherapy; 2022, 9, 1; 36-44
2353-7175
2543-9987
Pojawia się w:
OphthaTherapy
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Optimization of agarase production by alkaline Pseudomonas aeruginosa ZSL-2 using Taguchi experimental design
Autorzy:
Ziayoddin, M.
Lalitha, J.
Shinde, M.
Powiązania:
https://bibliotekanauki.pl/articles/11008.pdf
Data publikacji:
2014
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
optimization
agarase
production
alkaline
Pseudomonas aeruginosa
Taguchi method
Opis:
The culture conditions for the production of extracellular agarase by Pseudomonas aeruginosa ZSL-2 were optimized using One-Factor-At-A-Time combined with orthogonal array design. One-Factor-At-A-Time method investigates the effect of time, temperature, NaCl, carbon sources, nitrogen sources and pH on agarase production. The optimized culture conditions obtained from the statistical analysis were temperature of 30 °C, pH 8.5, NH4NO3 2 g L-1 and agar 3 g L-1. The L9 orthogonal array design was used to select the fermentation parameters influencing the yield of agarase. The order of the factors affecting the fermentation process was found to be NH4NO3 > pH > agar > temperature, with temperature playing a significant role on the agarase production (p < 0.10). The higher yields than those in basal media culture were obtained in the final optimized medium with activity of 0.439 ± 0.013 U ml-1. Extracellular agarase hydrolysed agar into a range of oligosaccharides which were analysed by LC-ESI-MS spectrometry as anhydrogalactose, galactose, agarobiose, agarotetrose and agarohexaose.
Źródło:
International Letters of Natural Sciences; 2014, 12, 2
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Złośliwe zapalenie ucha zewnętrznego – opis przypadku
Malignant Otitis Externa – case report
Autorzy:
Lepka, Paulina
Katkowska, Anna
Barnaś, Szczepan
Powiązania:
https://bibliotekanauki.pl/articles/1033526.pdf
Data publikacji:
2018
Wydawca:
Oficyna Wydawnicza Mediton
Tematy:
Pseudomonas aeruginosa
cukrzyca
zapalenie ucha zewnętrznego
złośliwe zapalenie ucha zewnętrznego
Opis:
Malignant Otitis Externa is a rare infection in the external auditory canal, involving the temporal bone, that can be life-threatening for the patient. Immunocompromised elderly patients with diabetes are especially prone to the condition. Mortality rate used to be nearly 50%, whereas nowadays, due to long-term antibiotic therapy including fluoroquinolones, third and fourthgeneration cephalosporins, gentamicin and topical therapy, it has been reduced to about 15%. Diagnosis is based on laboratory tests, imaging studies and microbiological testing. The main etiological factor inducing the development of malignant external otitis is Pseudomonas aeruginosa. The paper presents a case report of a 71-year-old man with uncontrolled diabetes and malignant otitis externa. We describe the way an accurate diagnosis was established, together with the process of treatment, which was completed with clinical improvement, both systemic and local. The patient remains under care of the ENT, endocrine and neurological department. This report summarises information about the malignant otitis externa.
„Złośliwe” zapalenie ucha zewnętrznego (malignant external otitis) jest rzadko występującym zakażeniem przewodu słuchowego zewnętrznego i kości podstawy czaszki mogącym zagrażać życiu chorego. Schorzenie to występuje głównie u osób starszych z zaburzeniami odporności oraz cukrzycą. Śmiertelność wynosiła niegdyś około 50%, aktualnie dzięki stosowaniu wła- ściwej, długotrwałej antybiotykoterapii m.in fluorochinolonów, cefalosporyn III i IV generacji, gentamycyny i dzięki leczeniu miejscowemu wynosi około 15%. Rozpoznanie opiera się na badaniach laboratoryjnych, obrazowych i mikrobiologicznych. Czynnikiem etiologicznym izolowanym najczęściej jest Pseudomonas aeruginosa. Przedstawiono przypadek procesu diagnostycznego u 71-letniego pacjenta z niewyrównaną cukrzycą, ze złośliwym zapaleniem ucha zewnętrznego oraz proces terapeutyczny u tego chorego, zakończony poprawą stanu ogólnego i miejscowego. Pacjent pozostaje pod kontrolą w poradniach ambulatoryjnych: laryngologicznej, endokrynologicznej, neurologicznej. Niniejsze opracowanie będzie podsumowaniem wiadomości o złośliwym zapaleniu ucha zewnętrznego.
Źródło:
Otorynolaryngologia - przegląd kliniczny; 2018, 17, 3; 118-123
1643-658X
Pojawia się w:
Otorynolaryngologia - przegląd kliniczny
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antibacterial activity of modified zinc oxide nanoparticles against Pseudomonas aeruginosa isolates of burn infections
Autorzy:
Aysa, Noor Hadi
Salman, Halah Dawood
Powiązania:
https://bibliotekanauki.pl/articles/1191368.pdf
Data publikacji:
2016
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Antimicrobial activity
Pseudomonas aeruginosa
ZnO
burn infection
nanoparticles
surface modification
Opis:
In this research antimicrobial activity of nanoparticles ZnO on perilous bacteria such as Pseudomonas aeruginosa was evaluated. P. aeruginosa is important pathogen that caused burn wound infections as it is multi-drug resistant and has several virulence factors. Fifteen samples of P. aeruginosa were collected from patients who suffering from Burn infections in Al-Hilla teaching hospital burn unit with the age range between (7-80) years old for both genders. After collecting burn samples, the diagnosis and characterization were performed by culturing and biochemical tests. ZnO NPs were synthesized by chemical method, Zinc oxide nanoparticles are well-known to be one of the multifunctional inorganic compounds which are widely used in medical applications. This study aims to prepare ZnO nanoparticles with particle size ranging from 23-29  nm. In the present study, surface modification of ZnO nanoparticles was performed, and influence of modification of the structure and morphological properties was investigated. The resulting nanoparticles were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and atomic force (AFM). Zinc oxide nanoparticles with the average diameter of about 29 nm were modified with an oleic acid to exert more compatibility. From the results obtained it is suggested that modified ZnO-nanoparticles could be used effectively in safety environmental and medical applications. Antibacterial activity for nanoparticle ZnO against P. aeruginosa isolates was measured by: Agar Diffusion Technique and Minimum Inhibitory Concentration (MIC)/Minimum bactericidal Concentration (MBC) with microdilution. The best zone of inhibition was (35.5mm) at a concentration of 40 μg/ml of nano-ZnO in one strain of P. aeruginosa while the lowest inhibition zone was (16 mm) at a concentration of 20 μg/ml of nano ZnO in one strain also. In addition, all P. aeruginosa isolates were completely inhibited at the concentration of 3.7 μg/ml of nano-ZnO (MIC) but no significant antibacterial activity was observed at concentrations less than 1.8 μg/ml of nano-ZnO and the (MBC) was same as MIC (3.7 μg/ml) for all P.aeruginosa isolates.
Źródło:
World Scientific News; 2016, 33; 1-14
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł

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