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    Wyświetlanie 1-8 z 8
    Tytuł:
    Wild game as a reservoir of Anaplasma phagocytophilum in North-Western Poland
    Autorzy:
    Adamska, M
    Skotarczak, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/840225.pdf
    Data publikacji:
    2007
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    parasite
    Polska
    reservoir
    roe deer
    bacteria
    Anaplasma phagocytophilum
    polymerase chain reaction
    intracellular parasite
    tick
    Źródło:
    Annals of Parasitology; 2007, 53, 2
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wild game as a reservoir of Anaplasma phagocytophilum in North-Western Poland
    Autorzy:
    Adamska, M.
    Skotarczak, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/2143862.pdf
    Data publikacji:
    2007
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    parasite
    Polska
    reservoir
    roe deer
    bacteria
    Anaplasma phagocytophilum
    polymerase chain reaction
    intracellular parasite
    tick
    Opis:
    Background. The aim of the study was to determine the role of game animals as reservoirs of Anaplasma phagocytophilum, a bacteria species transmitted by Ixodes ricinus ticks, from north−western Poland (Zachodniopomorskie vovoidship). The area under question is endemic for A. phagocytophilum. Material and methods. Blood and spleen samples were taken from 72 roe deer between April and December 2003. Animals culled during winter did not harbour ticks, on the other hand 155 individuals of Ixodes ricinus were collected from 35 of 43 animals taken during spring. We tested all samples for A. phagocytophilum by PCR amplification of the msp2 gene. An individual was considered infected if pathogens were detected in at least one isolate (blood or a tissue sample). Results. DNA from A. phagocytophilum was not found in isolates from ticks collected from the animals. The general level of infection for the roe deer was 31.94% (23/72). DNA of A. phagocytophilum was most commonly detected in blood samples; only in three cases was anaplasma DNA detected in spleen and not in blood. Ruminants seem to be the most competent reservoir for A. phagocytophilum in north−western Poland.
    Źródło:
    Wiadomości Parazytologiczne; 2007, 53, 2; 103-107
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Occurrence of Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Babesia microti in Ixodes ricinus ticks collected from selected areas of Opolskie Province in south-west Poland
    Autorzy:
    Asman, M.
    Witecka, J.
    Solarz, K.
    Zwonik, A.
    Szilman, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/2085175.pdf
    Data publikacji:
    2019
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Ixodes ricinus
    Opolskie Province
    Polska
    Borrelia burgdorferi sensu lato
    Anaplasma phagocytophilum
    Babesia microti
    Borrelia burgdorferi sensu lato genospecies
    Opis:
    Introduction. Ticks (Acari: Ixodida) are vectors and/or reservoirs of many pathogens, i.e. Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Babesia microti. These pathogens are ethiological agents of such diseases as Lyme borreliosis, human granulocytic anaplasmosis and human babesiosis. Objective. The aim of the study was to evaluate the role of the Ixodes ricinus in the transmission of Borrelia burgdorferi sensu lato, Borrelia afzelii, Borrelia garinii, Borrelia burgdorferi sensu stricto, Anaplasma phagocytophilum and Babesia microti in Opolskie Province in Poland. Materials and method. DNA from 222 ticks was isolated by the ammonia method. The pair of primers specific to the flagelline gene was used to detect of B. burgdorferi s. l. To detect of genospecies of this spirochete, three pairs of internal primers were used. In turn, two pairs of primers specific to the 16S rDNA gene and the 18S rRNA were used, respectively, for the detection of A. phagocytophilum and B. microti. Borrelia burgdorferi s. l., A. phagocytophilum, and B. microti were detected in 4.5%, 2.7% and 5.4% of examined ticks, respectively. Results and conclusions. Of the ten ticks infected with B. burgdorferi s. l., B. afzelii was found in seven, undefinied genospecies in two, and mixed infection with B. afzelii and B. burgdorferi s. s. in one. The study demonstrated the potential risk of exposure of humans and animals to infections of B. burgdorferi s. l., A. phagocytophilum and B. microti in the examined area of Poland.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2019, 26, 4; 544-547
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Co-infection with Borrelia species and other tick-borne pathogens in humans: two cases from Poland
    Autorzy:
    Welc-Faleciak, R
    Hildebrandt, A.
    Sinski, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/49402.pdf
    Data publikacji:
    2010
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    human disease
    human health
    tick-borne disease
    tick-borne pathogen
    man
    Borrelia
    Anaplasma phagocytophilum
    Babesia
    co-infection
    Polska
    prevalence
    Ixodes ricinus
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2010, 17, 2; 309-313
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Anaplasma phagocytophilum infection of red foxes [Vulpes vulpes]
    Autorzy:
    Karbowiak, G
    Vichova, B.
    Majlathova, V.
    Hapunik, J.
    Pet'ko, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/50073.pdf
    Data publikacji:
    2009
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Polska
    Mazovia region
    parasite
    Anaplasma phagocytophilum
    infection
    animal disease
    red fox
    Vulpes vulpes
    16S rRNA gene
    msp4 gene
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2009, 16, 2; 299-300
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Identification of the piroplasms isolated from horses with clinical piroplasmosis in Poland
    Identyfikacja piroplazm izolowanych od koni w Polsce w klinicznych przypadkach choroby
    Autorzy:
    Adaszek, L.
    Gorna, M.
    Krzysiak, M.
    Adaszek, M.
    Garbal, M.
    Winiarczyk, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/838784.pdf
    Data publikacji:
    2011
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    Anaplasma
    Babesia
    Ehrlichia
    Polska
    Theileria
    animal disease
    disease symptom
    diseased animal
    horse
    identification
    piroplasm
    piroplasmosis
    polymerase chain reaction
    ricketsia
    treatment
    tick-borne disease
    Źródło:
    Annals of Parasitology; 2011, 57, 1
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Identification of the piroplasms isolated from horses with clinical piroplasmosis in Poland
    Identyfikacja piroplazm izolowanych od koni w Polsce w klinicznych przypadkach choroby
    Autorzy:
    Adaszek, L.
    Górna, M.
    Krzysiak, M.
    Adaszek, M.
    Garbal, M.
    Winiarczyk, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/2143301.pdf
    Data publikacji:
    2011
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    Anaplasma
    Babesia
    Ehrlichia
    Polska
    Theileria
    animal disease
    disease symptom
    diseased animal
    horse
    identification
    piroplasm
    piroplasmosis
    polymerase chain reaction
    ricketsia
    treatment
    tick-borne disease
    Opis:
    The study was aimed at determining the cause of the diseases in three horses exhibiting symptoms of fever, ataxia, mucus membrane paleness, haematuria and thrombocytopenia. The PCR technique revealed the presence in the blood of 18S RNA Babesia/Theileria spp. genetic material. DNA amplification using primers RLB F2 and RLB R2 produced 430 bp size products. The sequences of these PCR products demonstrated a 95.6–97.5% similarity with the sequence of the fragment of 18S RNA Babesia equi, gene number DQ287951 in the GenBank. The treatment utilizing the subcutaneous application of the imidocarb resulted in gradual recovery of the diseased animals.
    Źródło:
    Wiadomości Parazytologiczne; 2011, 57, 1; 21-26
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Prevalence of Anaplasma phagocytophilum in Ixodes ricinus ticks determined by polymerase chain reaction with two pairs of primers detecting 16S rRNA and ankA genes
    Autorzy:
    Chmielewska-Badora, J
    Zwolinski, J.
    Cisak, E.
    Wojcik-Fatla, A.
    Buczek, A.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/51023.pdf
    Data publikacji:
    2007
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Lublin region
    Polska
    pathogen
    Ixodes ricinus
    Eastern Poland
    tick
    determination
    animal pathogen
    human pathogen
    ankA gene
    16S rRNA gene
    granulocytic anaplasmosis
    bacteria
    Anaplasma phagocytophilum
    polymerase chain reaction
    Opis:
    A total of 684 Ixodes ricinus ticks (321 nymphs, 184 males, and 179 females) were collected by fl agging lower vegetation in 6 forest districts located on the territory of Lublin province (eastern Poland). Ticks were examined by polymerase chain reaction (PCR) method for the presence of Anaplasma phagocytophilum DNA with two pairs of primers: EHR521/EHR747 for detecting 16S rRNA gene, and LA6/LA1 for detecting ankA gene. To study the relationship between infection in ticks and people occupationally exposed to tick bite, blood serum samples of 261 forestry workers employed in the same forest districts were examined by immunofl uorescence method for the presence of specifi c antibodies against A. phagocytophilum. A total of 70 ticks out of 684 examined (10.2%) showed the presence of A. phagocytophilum 16S rRNA gene. The prevalence of infection was signifi cantly dependent on tick’s stage (χ2=49.2, p<0.00001) and geographical locality (χ2=34.4, p<0.00001). The percentage of I. ricinus females infected with A. phagocytophilum (24.6%) was signifi cantly greater compared to males (6.5%) and nymphs (4.4%) (p<0.00001). Only 19 ticks out of 684 examined (2.8%) showed the presence of A. phagocytophilum ankA gene, signifi cantly less compared to 16S rRNA gene (p<0.00001). The prevalence of infection demonstrated by the presence of ankA gene was also signifi cantly dependent on tick’s stage (χ2=23.6, p<0.00001) but not on locality (χ2=9.8, p=0.082). A signifi cant correlation was found between the presence of A. phagocytophilum 16S rRNA gene in I. ricinus female ticks from the particular forest districts and the serologic response to A. phagocytophilum of forestry workers employed in the same districts (p<0.05). No signifi cant correlation was found between the presence of A. phagocytophilum ankA gene in I. ricinus ticks and serologic response of exposed workers. In conclusion, detection of A. phagocytophilum infection in ticks by PCR with the use of EHR521/EHR747 primers detecting 16S rRNA gene is signifi cantly more sensitive compared to LA6/LA1 primers detecting ankA gene.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2007, 14, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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