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    Wyświetlanie 1-15 z 15
    Tytuł:
    The occurrence of Toxoplasma gondii and Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from East Poland with the use of PCR
    Autorzy:
    Sroka, J
    Szymanska, J.
    Wojcik-Fatla, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/51137.pdf
    Data publikacji:
    2009
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Polska
    tick
    Ixodes ricinus
    Toxoplasma gondii
    Borrelia burgdorferi
    occurrence
    polymerase chain reaction
    genotyping
    B1 gene
    human population
    infection
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2009, 16, 2; 313-319
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Hantavirus RNA not detected in Ixodes ricinus ticks
    Autorzy:
    Wojcik-Fatla, A.
    Zajac, V.
    Knap, J.P.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/49890.pdf
    Data publikacji:
    2011
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    hantavirus
    RNA
    Ixodes ricinus
    tick
    epidemiology
    polymerase chain reaction
    Polska
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2011, 18, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Seroepidemiological study of Lyme borreliosis among forestry workers in Southern Poland
    Autorzy:
    Buczek, A
    Rudek, A.
    Bartosik, K.
    Szymanska, J.
    Wojcik-Fatla, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/49580.pdf
    Data publikacji:
    2009
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Lyme borreliosis
    borreliosis
    serology
    ELISA test
    forestry
    worker
    Polska
    forestry worker
    tick-borne disease
    diagnosis
    antibody
    treatment
    Borrelia burgdorferi
    tick
    immunoglobulin
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2009, 16, 2; 257-261
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Study on Giardia duodenalis and Cryptosporidium spp. infection in veterinarians in Poland
    Autorzy:
    Wójcik-Fatla, A.
    Sroka, J.
    Zając, V.
    Zwoliński, J.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2081928.pdf
    Data publikacji:
    2018
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Giardia duodenalis
    Cryptosporidium spp.
    veterinarians
    Polska
    Opis:
    The main aim of the study was to determine the risk of two selected zoonosis infections caused by Giardia duodenalis and Cryptosporidium spp. among an occupational group of veterinarians in Poland. Two hundred and ninety-seven samples of stool were tested for the presence of Giardia cysts and Cryptosporidium oocysts using Direct Fluorescent Assay (DFA). There were no positive results for Cryptosporidium. The presence of Giardia cysts was found in two samples of faeces (0.67%). The risk with regard to the parasites Giardia duodenalis and Cryptosporidium spp. seems to be low among the group of veterinarians.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2018, 25, 4; 732-733
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Prevalence of tick-borne encephalitis virus [TBEV] in samples of raw milk taken randomly from cows, goats and sheep in Eastern Poland
    Autorzy:
    Cisak, E
    Wojcik-Fatla, A.
    Zajac, V.
    Sroka, J.
    Buczek, A.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/50068.pdf
    Data publikacji:
    2010
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    tick-borne encephalitis virus
    prevalence
    raw milk
    milk
    milk sample
    cow
    goat
    sheep
    Polska
    epidemiology
    antibody
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2010, 17, 2; 283-286
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Study on tick-borne rickettsiae in eastern Poland. I. Prevalence in Dermacentor reticulatus (Acari: Amblyommidae)
    Autorzy:
    Wojcik-Fatla, A.
    Cisak, E.
    Zajac, V.
    Sroka, J.
    Sawczyn, A.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/50634.pdf
    Data publikacji:
    2013
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Rickettsia
    Rickettsia raoultii
    Polska
    prevalence
    Dermacentor reticulatus
    tick
    Acari
    Amblyommidae
    Opis:
    Rickettsia spp. transmitted by ticks are classified mostly in the Spotted Fever Group Rickettsiae (SFGR). Numerous species of this group have been identified in Eurasia as emerging pathogens, but still little is known about their occurrence, effects on human health, and co-incidence with other tick-borne pathogens. The aim of the presented study was to determine the prevalence of Rickettsia spp. in adult Dermacentor reticulatus (Acari: Amblyommidae) ticks collected in Lublin province of eastern Poland using the PCR method. The infection rate of D. reticulatus with Rickettsia spp. was 53.0%. All except one rickettsial isolates showed 100% homology with Rickettsia raoultii. A high prevalence of R. raoultii in Dermacentor reticulatus ticks from eastern Poland suggests that the SFGR species should be considered as potential causative agents of tick-borne diseases in this area.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2013, 20, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Prevalence of Toxoplasma gondii infection in cats in southwestern Poland
    Autorzy:
    Sroka, J.
    Karamon, J.
    Dutkiewicz, J.
    Wójcik-Fatla, A.
    Zając, V.
    Cencek, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/2081746.pdf
    Data publikacji:
    2018
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Polska
    PCR
    seroprevalence
    Toxoplasma gondii
    IFAT
    cats
    oocysts
    coproscopy
    Opis:
    Objective. An assessment of the prevalence of Toxoplasma gondii infection in cats from southwestern Poland using serology, coproscopy and PCR methods. Materials and method. In total, 208 cats (139 females and 68 males), aged 0.5–12 years (mean=2.6) from 25 localities in southwestern Poland were examined by indirect immunofluorescence assay (IFAT) to estimate the T. gondii serological status. Faecal samples of 41 cats were examined for the presence of oocysts/DNA T. gondii by microscopy and Real-time/nested PCR. After flotation (with NaNO3), pellets from faecal samples were disrupted by 10 cycles of freezing (liquid nitrogen) and warming. DNA was extracted using QIamp DNA Stool Mini Kit (Qiagen), according to the manufacturer’s instruction. Results. The positive results in IFAT for anti-T. gondii IgG and IgM antibodies were found in 143 of 208 tested cats (68.8%). Among positive results, 14.5%, 34.1% and 51.4% were detected in titre ranges 128–512, 1,000–2,000 and ≥ 4,000, respectively. In 23.1% of cat sera anti- T. gondii IgM antibodies were found. The prevalence of anti-Toxoplasma antibodies was significantly greater in older cats (>1 year) (83.5%) than in younger cats (48.3%) (P<0.05), in females (74.1%) than in males (58.8%) (P<0.05), and in cats kept outdoors than indoors (69.7% vs. 16.7%) (P<0.01). Among the 41 faecal samples examined, the presence of structures resembling T. gondii oocysts was found in 2 samples (4.9%), and for one of these samples (2.4% of the total) the result was also confirmed by PCR. Conclusions. T. gondii infection in domestic cats is highly prevalent in southwestern Poland. Information on the prevalence of infection in cats can be useful for assessing T. gondii environmental contamination and the risk for public health.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2018, 25, 3; 1-5
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Study on tick-borne rickettsiae in eastern Poland. II. Serological response of occupationally exposed populations
    Autorzy:
    Zajac, V.
    Wojcik-Fatla, A.
    Cisak, E.
    Sroka, J.
    Sawczyn, A.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/51448.pdf
    Data publikacji:
    2013
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    tick
    Rickettsia
    Polska
    serological response
    occupational exposure
    population
    serology
    ELISA test
    forestry worker
    agricultural worker
    Opis:
    A group of 150 persons living in the Lublin province of eastern Poland and occupationally exposed to tick bite were examined by the immunoenzymatic ELISA test for the presence of antibodies against tick-borne Spotted Fever Group (SFG) rickettsiae. The group consisted of 75 forestry workers employed in 3 forest inspectorates and 75 agricultural workers living in 2 villages. As a control group, 43 urban dwellers living in the city of Lublin and not occupationally exposed to tick bite were examined. Among 150 persons occupationally exposed to tick bite, the presence of antibodies against SFG rickettsiae was found in 54 (36.0% of the total). In the control group, the frequency of positive findings was only 4.7%, being significantly smaller compared to the exposed group (p=0.0001). Within the exposed group, the percentage of positive results in forestry workers (50.7%) was greater than in agricultural workers (21.3%); the difference was statistically significant (p=0.0002). Also within this group, the frequency of positive findings in males(46.5%) was significantly greater than in females (21.9%) (p=0.0029). In the exposed group, the positive results tended to increase with the age of the examined persons. However, a significant relationship between age and positive findings was found only in forestry workers (c2=14.207, p=0.00264), but not in agricultural workers and total exposed workers. The frequencies of positive results in forestry workers varied significantly depending on place of work (c2=11.271, p=0.00357). Similarly, the difference between the positive reactions in agricultural workers living in 2 villages proved to be significant (34.2% vs. 8.1%; p=0.0074). The obtained results indicate that people occupationally exposed to tick bite and living in the area of eastern Poland where over half of Dermacentor reticulatus ticks harbour SFG rickettsiae, are under significantly increased risk of infection with these rickettsiae.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2013, 20, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Preliminary study on the occurrence of Toxoplasma gondii in Ixodes ricinus ticks from north-western Poland with the use of PCR
    Autorzy:
    Sroka, J
    Wojcik-Fatla, A.
    Zwolinski, J.
    Zajac, V.
    Sawczuk, M.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/51615.pdf
    Data publikacji:
    2008
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    polymerase chain reaction
    parasite
    genotyping
    Polska
    tick
    toxoplasmosis
    Ixodes ricinus
    B1 gene
    zoonotic disease
    occurrence
    Toxoplasma gondii
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2008, 15, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Prevalence of Anaplasma phagocytophilum in Ixodes ricinus ticks determined by polymerase chain reaction with two pairs of primers detecting 16S rRNA and ankA genes
    Autorzy:
    Chmielewska-Badora, J
    Zwolinski, J.
    Cisak, E.
    Wojcik-Fatla, A.
    Buczek, A.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/51023.pdf
    Data publikacji:
    2007
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Lublin region
    Polska
    pathogen
    Ixodes ricinus
    Eastern Poland
    tick
    determination
    animal pathogen
    human pathogen
    ankA gene
    16S rRNA gene
    granulocytic anaplasmosis
    bacteria
    Anaplasma phagocytophilum
    polymerase chain reaction
    Opis:
    A total of 684 Ixodes ricinus ticks (321 nymphs, 184 males, and 179 females) were collected by fl agging lower vegetation in 6 forest districts located on the territory of Lublin province (eastern Poland). Ticks were examined by polymerase chain reaction (PCR) method for the presence of Anaplasma phagocytophilum DNA with two pairs of primers: EHR521/EHR747 for detecting 16S rRNA gene, and LA6/LA1 for detecting ankA gene. To study the relationship between infection in ticks and people occupationally exposed to tick bite, blood serum samples of 261 forestry workers employed in the same forest districts were examined by immunofl uorescence method for the presence of specifi c antibodies against A. phagocytophilum. A total of 70 ticks out of 684 examined (10.2%) showed the presence of A. phagocytophilum 16S rRNA gene. The prevalence of infection was signifi cantly dependent on tick’s stage (χ2=49.2, p<0.00001) and geographical locality (χ2=34.4, p<0.00001). The percentage of I. ricinus females infected with A. phagocytophilum (24.6%) was signifi cantly greater compared to males (6.5%) and nymphs (4.4%) (p<0.00001). Only 19 ticks out of 684 examined (2.8%) showed the presence of A. phagocytophilum ankA gene, signifi cantly less compared to 16S rRNA gene (p<0.00001). The prevalence of infection demonstrated by the presence of ankA gene was also signifi cantly dependent on tick’s stage (χ2=23.6, p<0.00001) but not on locality (χ2=9.8, p=0.082). A signifi cant correlation was found between the presence of A. phagocytophilum 16S rRNA gene in I. ricinus female ticks from the particular forest districts and the serologic response to A. phagocytophilum of forestry workers employed in the same districts (p<0.05). No signifi cant correlation was found between the presence of A. phagocytophilum ankA gene in I. ricinus ticks and serologic response of exposed workers. In conclusion, detection of A. phagocytophilum infection in ticks by PCR with the use of EHR521/EHR747 primers detecting 16S rRNA gene is signifi cantly more sensitive compared to LA6/LA1 primers detecting ankA gene.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2007, 14, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A small-scale survey of hantavirus in mammals from eastern Poland
    Autorzy:
    Wojcik-Fatla, A.
    Zajac, V.
    Knap, J.P.
    Sroka, J.
    Cisak, E.
    Sawczyn, A.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/51577.pdf
    Data publikacji:
    2013
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    hantavirus
    Bunyaviridae
    mammal
    small mammal
    Microtus agrestis
    Myodes glareolus
    Sorex araneus
    epidemiology
    polymerase chain reaction
    flood
    Polska
    Opis:
    Samples of 30 dead small mammals each were collected on area ‘A’ located in eastern Poland which is exposed to flooding by the Vistula river, and on the area ‘B’, also located in eastern Poland but not exposed to flooding. Kidneys and livers of the mammals were examined by the PCR and nested PCR methods for the presence of hantavirus RNA. Out of 7 species of small mammals examined, the presence of hantaviruses was detected in 4 of them. Hantavirus prevalence was low in Apodemus agrarius (2.6%), the most numerous mammal species, whereas in the remaining 3 positive species (Microtus agrestis, Myodes glareolus, Sorex araneus) this was 12.5–100%. The presence of hantaviruses was detected only in the animals found on area ‘A’ exposed to flooding, and their prevalence was statistically greater compared to area ‘B’ not exposed to flooding (16.7% vs. 0%, p=0.0345). The overall positivity of the examined small mammals population from the areas ‘A’ and ‘B’ was 8.3%. The sequence analysis of the samples positive for hantavirus proved that the amplified products showed 77–86% homology with the L segment sequence of hantavirus Fusong-Mf-731 isolated from Microtus fortis in China. The presented study is the first to demonstrate the occurrence of hantavirus infection in small mammals from eastern Poland, and the first to demonstrate the significant relationship between flooding and the prevalence of hantaviruses in small mammals.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2013, 20, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Study on Lyme borreliosis focus in the Lublin region [eastern Poland]
    Autorzy:
    Cisak, E
    Chmielewska-Badora, J.
    Zwolinski, J.
    Wojcik-Fatla, A.
    Zajac, V.
    Skorska, C.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/51535.pdf
    Data publikacji:
    2008
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    borreliosis
    Polska
    rural inhabitant
    Lyme borreliosis
    tick
    seroepidemiology
    Ixodes ricinus
    Borrelia burgdorferi
    clinical case
    Lublin region
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2008, 15, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Toxoplasma gondii infection in selected species of free-living animals in Poland
    Autorzy:
    Sroka, J.
    Karamon, J.
    Wójcik-Fatla, A.
    Dutkiewicz, J.
    Bilska-Zając, E.
    Zając, V.
    Piotrowska, W.
    Cencek, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/2085130.pdf
    Data publikacji:
    2019
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Polska
    Toxoplasma gondii
    nested PCR
    genotyping
    free-living animals
    Opis:
    Introduction and objective. Free-living animals can play an important role as a reservoir of Toxoplasma gondi;, however, data concerning this issue in Poland are still limited.The aim of study was to assess the occurrence of T. gondii infection by using molecular methods in free-living animals in selected regions of Poland. Materials and method. Tissues samples of 396 animals (foxes, muskrats, birds, martens, badgers, polecats, raccoons, minks, raccoon dogs, otters, small rodents and insectivores, and grass snakes were collected from various regions of Poland. After samples digestion, DNA was isolated using QIAmp DNA Mini Kit (Qiagen). DNA extraction from small rodents and insectivores samples was performed without digestion. Next, nested PCR (B1 gene) and, for a part of nested PCR positive amplicons, RFLP PCR, were performed according to the method by Grigg and Boothroyd (2001). The other part of nested PCR positive DNA isolates were genotyped using 5 genetic markers: SAG1, SAG2 (5’- and 3’), SAG3, BTUB and GRA6, based on the method by Dubey et al. (2006). These PCR products were sequenced and compared with the NCBI database using Blast. Results. In total, in 50 of the 396 examined animals DNA of T. gondii was detected (12.6%). The highest percentages of positive results in PCR was obtained in martens (40.9%) and badgers (38.5%), lower in birds (27.3%) and the lowest in foxes (7.4%). The RFLP and multilocus PCR analysis showed the dominance of T. gondii clonal type II (or II/III). Conclusions. The results of this study indicate the frequent T. gondii infection among free-living animals in Poland, especially martens and badgers, which may indirectly indicate that these animals contribute to the spread of the parasite in the sylvatic environment in Poland. The genotyping analysis showed the dominance of T. gondii clonal type II (or II/III).
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2019, 26, 4; 656-660
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Study on prevalence of Toxoplasma gondii infection in free-living animals in Poland
    Autorzy:
    Sroka, J.
    Karamon, J.
    Bilska-Zajac, E.
    Stojecki, K.
    Rozycki, M.
    Kusyk, P.
    Wojcik-Fatla, A.
    Zajac, V.
    Cencek, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/6474.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    Toxoplasma gondii
    animal infection
    prevalence
    free living animal
    Polska
    Źródło:
    Annals of Parasitology; 2016, 62, Suppl.
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Occupational exposure to organic dust, microorganisms, endotoxin and peptidoglycan among plants processing workers in Poland
    Autorzy:
    Gora, A
    Mackiewicz, B.
    Krawczyk, P.
    Golec, M.
    Skorska, C.
    Sitkowska, J.
    Cholewa, G.
    Larsson, L.
    Jarosz, M.
    Wojcik-Fatla, A.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/50063.pdf
    Data publikacji:
    2009
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    occupational exposure
    organic dust
    dust
    microorganism
    endotoxin
    plant processing
    worker
    Polska
    peptidoglycan
    bioaerosol
    agricultural industry
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2009, 16, 1; 143-150
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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