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Tytuł:
The occurrence of Toxoplasma gondii and Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from East Poland with the use of PCR
Autorzy:
Sroka, J
Szymanska, J.
Wojcik-Fatla, A.
Powiązania:
https://bibliotekanauki.pl/articles/51137.pdf
Data publikacji:
2009
Wydawca:
Instytut Medycyny Wsi
Tematy:
Polska
tick
Ixodes ricinus
Toxoplasma gondii
Borrelia burgdorferi
occurrence
polymerase chain reaction
genotyping
B1 gene
human population
infection
Źródło:
Annals of Agricultural and Environmental Medicine; 2009, 16, 2; 313-319
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Hantavirus RNA not detected in Ixodes ricinus ticks
Autorzy:
Wojcik-Fatla, A.
Zajac, V.
Knap, J.P.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/49890.pdf
Data publikacji:
2011
Wydawca:
Instytut Medycyny Wsi
Tematy:
hantavirus
RNA
Ixodes ricinus
tick
epidemiology
polymerase chain reaction
Polska
Źródło:
Annals of Agricultural and Environmental Medicine; 2011, 18, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Study on Giardia duodenalis and Cryptosporidium spp. infection in veterinarians in Poland
Autorzy:
Wójcik-Fatla, A.
Sroka, J.
Zając, V.
Zwoliński, J.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/2081928.pdf
Data publikacji:
2018
Wydawca:
Instytut Medycyny Wsi
Tematy:
Giardia duodenalis
Cryptosporidium spp.
veterinarians
Polska
Opis:
The main aim of the study was to determine the risk of two selected zoonosis infections caused by Giardia duodenalis and Cryptosporidium spp. among an occupational group of veterinarians in Poland. Two hundred and ninety-seven samples of stool were tested for the presence of Giardia cysts and Cryptosporidium oocysts using Direct Fluorescent Assay (DFA). There were no positive results for Cryptosporidium. The presence of Giardia cysts was found in two samples of faeces (0.67%). The risk with regard to the parasites Giardia duodenalis and Cryptosporidium spp. seems to be low among the group of veterinarians.
Źródło:
Annals of Agricultural and Environmental Medicine; 2018, 25, 4; 732-733
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Kierunki rozwoju technologii i mechanizacji pozyskiwania drewna w Polsce
Napravlenija razvitija tekhnologii i mekhanizacii zagotovki drevesiny w Polshe
Directions of development of wood harvesting technology and mechanization in Poland
Autorzy:
Komorowski, J.
Rzadkowski, S.
Suwała, M.
Wójcik, T.
Powiązania:
https://bibliotekanauki.pl/articles/1178305.pdf
Data publikacji:
1988
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
lesnictwo
Panstwowe Gospodarstwo Lesne Lasy Panstwowe
pozyskiwanie drewna
technologia pozyskiwania
mechanizacja prac lesnych
kierunki rozwoju
Polska
Źródło:
Sylwan; 1988, 132, 02
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prevalence of Toxoplasma gondii infection in cats in southwestern Poland
Autorzy:
Sroka, J.
Karamon, J.
Dutkiewicz, J.
Wójcik-Fatla, A.
Zając, V.
Cencek, T.
Powiązania:
https://bibliotekanauki.pl/articles/2081746.pdf
Data publikacji:
2018
Wydawca:
Instytut Medycyny Wsi
Tematy:
Polska
PCR
seroprevalence
Toxoplasma gondii
IFAT
cats
oocysts
coproscopy
Opis:
Objective. An assessment of the prevalence of Toxoplasma gondii infection in cats from southwestern Poland using serology, coproscopy and PCR methods. Materials and method. In total, 208 cats (139 females and 68 males), aged 0.5–12 years (mean=2.6) from 25 localities in southwestern Poland were examined by indirect immunofluorescence assay (IFAT) to estimate the T. gondii serological status. Faecal samples of 41 cats were examined for the presence of oocysts/DNA T. gondii by microscopy and Real-time/nested PCR. After flotation (with NaNO3), pellets from faecal samples were disrupted by 10 cycles of freezing (liquid nitrogen) and warming. DNA was extracted using QIamp DNA Stool Mini Kit (Qiagen), according to the manufacturer’s instruction. Results. The positive results in IFAT for anti-T. gondii IgG and IgM antibodies were found in 143 of 208 tested cats (68.8%). Among positive results, 14.5%, 34.1% and 51.4% were detected in titre ranges 128–512, 1,000–2,000 and ≥ 4,000, respectively. In 23.1% of cat sera anti- T. gondii IgM antibodies were found. The prevalence of anti-Toxoplasma antibodies was significantly greater in older cats (>1 year) (83.5%) than in younger cats (48.3%) (P<0.05), in females (74.1%) than in males (58.8%) (P<0.05), and in cats kept outdoors than indoors (69.7% vs. 16.7%) (P<0.01). Among the 41 faecal samples examined, the presence of structures resembling T. gondii oocysts was found in 2 samples (4.9%), and for one of these samples (2.4% of the total) the result was also confirmed by PCR. Conclusions. T. gondii infection in domestic cats is highly prevalent in southwestern Poland. Information on the prevalence of infection in cats can be useful for assessing T. gondii environmental contamination and the risk for public health.
Źródło:
Annals of Agricultural and Environmental Medicine; 2018, 25, 3; 1-5
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prevalence of Anaplasma phagocytophilum in Ixodes ricinus ticks determined by polymerase chain reaction with two pairs of primers detecting 16S rRNA and ankA genes
Autorzy:
Chmielewska-Badora, J
Zwolinski, J.
Cisak, E.
Wojcik-Fatla, A.
Buczek, A.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/51023.pdf
Data publikacji:
2007
Wydawca:
Instytut Medycyny Wsi
Tematy:
Lublin region
Polska
pathogen
Ixodes ricinus
Eastern Poland
tick
determination
animal pathogen
human pathogen
ankA gene
16S rRNA gene
granulocytic anaplasmosis
bacteria
Anaplasma phagocytophilum
polymerase chain reaction
Opis:
A total of 684 Ixodes ricinus ticks (321 nymphs, 184 males, and 179 females) were collected by fl agging lower vegetation in 6 forest districts located on the territory of Lublin province (eastern Poland). Ticks were examined by polymerase chain reaction (PCR) method for the presence of Anaplasma phagocytophilum DNA with two pairs of primers: EHR521/EHR747 for detecting 16S rRNA gene, and LA6/LA1 for detecting ankA gene. To study the relationship between infection in ticks and people occupationally exposed to tick bite, blood serum samples of 261 forestry workers employed in the same forest districts were examined by immunofl uorescence method for the presence of specifi c antibodies against A. phagocytophilum. A total of 70 ticks out of 684 examined (10.2%) showed the presence of A. phagocytophilum 16S rRNA gene. The prevalence of infection was signifi cantly dependent on tick’s stage (χ2=49.2, p<0.00001) and geographical locality (χ2=34.4, p<0.00001). The percentage of I. ricinus females infected with A. phagocytophilum (24.6%) was signifi cantly greater compared to males (6.5%) and nymphs (4.4%) (p<0.00001). Only 19 ticks out of 684 examined (2.8%) showed the presence of A. phagocytophilum ankA gene, signifi cantly less compared to 16S rRNA gene (p<0.00001). The prevalence of infection demonstrated by the presence of ankA gene was also signifi cantly dependent on tick’s stage (χ2=23.6, p<0.00001) but not on locality (χ2=9.8, p=0.082). A signifi cant correlation was found between the presence of A. phagocytophilum 16S rRNA gene in I. ricinus female ticks from the particular forest districts and the serologic response to A. phagocytophilum of forestry workers employed in the same districts (p<0.05). No signifi cant correlation was found between the presence of A. phagocytophilum ankA gene in I. ricinus ticks and serologic response of exposed workers. In conclusion, detection of A. phagocytophilum infection in ticks by PCR with the use of EHR521/EHR747 primers detecting 16S rRNA gene is signifi cantly more sensitive compared to LA6/LA1 primers detecting ankA gene.
Źródło:
Annals of Agricultural and Environmental Medicine; 2007, 14, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Preliminary study on the occurrence of Toxoplasma gondii in Ixodes ricinus ticks from north-western Poland with the use of PCR
Autorzy:
Sroka, J
Wojcik-Fatla, A.
Zwolinski, J.
Zajac, V.
Sawczuk, M.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/51615.pdf
Data publikacji:
2008
Wydawca:
Instytut Medycyny Wsi
Tematy:
polymerase chain reaction
parasite
genotyping
Polska
tick
toxoplasmosis
Ixodes ricinus
B1 gene
zoonotic disease
occurrence
Toxoplasma gondii
Źródło:
Annals of Agricultural and Environmental Medicine; 2008, 15, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prevalence of tick-borne encephalitis virus [TBEV] in samples of raw milk taken randomly from cows, goats and sheep in Eastern Poland
Autorzy:
Cisak, E
Wojcik-Fatla, A.
Zajac, V.
Sroka, J.
Buczek, A.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/50068.pdf
Data publikacji:
2010
Wydawca:
Instytut Medycyny Wsi
Tematy:
tick-borne encephalitis virus
prevalence
raw milk
milk
milk sample
cow
goat
sheep
Polska
epidemiology
antibody
Źródło:
Annals of Agricultural and Environmental Medicine; 2010, 17, 2; 283-286
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Study on tick-borne rickettsiae in eastern Poland. I. Prevalence in Dermacentor reticulatus (Acari: Amblyommidae)
Autorzy:
Wojcik-Fatla, A.
Cisak, E.
Zajac, V.
Sroka, J.
Sawczyn, A.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/50634.pdf
Data publikacji:
2013
Wydawca:
Instytut Medycyny Wsi
Tematy:
Rickettsia
Rickettsia raoultii
Polska
prevalence
Dermacentor reticulatus
tick
Acari
Amblyommidae
Opis:
Rickettsia spp. transmitted by ticks are classified mostly in the Spotted Fever Group Rickettsiae (SFGR). Numerous species of this group have been identified in Eurasia as emerging pathogens, but still little is known about their occurrence, effects on human health, and co-incidence with other tick-borne pathogens. The aim of the presented study was to determine the prevalence of Rickettsia spp. in adult Dermacentor reticulatus (Acari: Amblyommidae) ticks collected in Lublin province of eastern Poland using the PCR method. The infection rate of D. reticulatus with Rickettsia spp. was 53.0%. All except one rickettsial isolates showed 100% homology with Rickettsia raoultii. A high prevalence of R. raoultii in Dermacentor reticulatus ticks from eastern Poland suggests that the SFGR species should be considered as potential causative agents of tick-borne diseases in this area.
Źródło:
Annals of Agricultural and Environmental Medicine; 2013, 20, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Study on Lyme borreliosis focus in the Lublin region [eastern Poland]
Autorzy:
Cisak, E
Chmielewska-Badora, J.
Zwolinski, J.
Wojcik-Fatla, A.
Zajac, V.
Skorska, C.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/51535.pdf
Data publikacji:
2008
Wydawca:
Instytut Medycyny Wsi
Tematy:
borreliosis
Polska
rural inhabitant
Lyme borreliosis
tick
seroepidemiology
Ixodes ricinus
Borrelia burgdorferi
clinical case
Lublin region
Źródło:
Annals of Agricultural and Environmental Medicine; 2008, 15, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A small-scale survey of hantavirus in mammals from eastern Poland
Autorzy:
Wojcik-Fatla, A.
Zajac, V.
Knap, J.P.
Sroka, J.
Cisak, E.
Sawczyn, A.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/51577.pdf
Data publikacji:
2013
Wydawca:
Instytut Medycyny Wsi
Tematy:
hantavirus
Bunyaviridae
mammal
small mammal
Microtus agrestis
Myodes glareolus
Sorex araneus
epidemiology
polymerase chain reaction
flood
Polska
Opis:
Samples of 30 dead small mammals each were collected on area ‘A’ located in eastern Poland which is exposed to flooding by the Vistula river, and on the area ‘B’, also located in eastern Poland but not exposed to flooding. Kidneys and livers of the mammals were examined by the PCR and nested PCR methods for the presence of hantavirus RNA. Out of 7 species of small mammals examined, the presence of hantaviruses was detected in 4 of them. Hantavirus prevalence was low in Apodemus agrarius (2.6%), the most numerous mammal species, whereas in the remaining 3 positive species (Microtus agrestis, Myodes glareolus, Sorex araneus) this was 12.5–100%. The presence of hantaviruses was detected only in the animals found on area ‘A’ exposed to flooding, and their prevalence was statistically greater compared to area ‘B’ not exposed to flooding (16.7% vs. 0%, p=0.0345). The overall positivity of the examined small mammals population from the areas ‘A’ and ‘B’ was 8.3%. The sequence analysis of the samples positive for hantavirus proved that the amplified products showed 77–86% homology with the L segment sequence of hantavirus Fusong-Mf-731 isolated from Microtus fortis in China. The presented study is the first to demonstrate the occurrence of hantavirus infection in small mammals from eastern Poland, and the first to demonstrate the significant relationship between flooding and the prevalence of hantaviruses in small mammals.
Źródło:
Annals of Agricultural and Environmental Medicine; 2013, 20, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Study on tick-borne rickettsiae in eastern Poland. II. Serological response of occupationally exposed populations
Autorzy:
Zajac, V.
Wojcik-Fatla, A.
Cisak, E.
Sroka, J.
Sawczyn, A.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/51448.pdf
Data publikacji:
2013
Wydawca:
Instytut Medycyny Wsi
Tematy:
tick
Rickettsia
Polska
serological response
occupational exposure
population
serology
ELISA test
forestry worker
agricultural worker
Opis:
A group of 150 persons living in the Lublin province of eastern Poland and occupationally exposed to tick bite were examined by the immunoenzymatic ELISA test for the presence of antibodies against tick-borne Spotted Fever Group (SFG) rickettsiae. The group consisted of 75 forestry workers employed in 3 forest inspectorates and 75 agricultural workers living in 2 villages. As a control group, 43 urban dwellers living in the city of Lublin and not occupationally exposed to tick bite were examined. Among 150 persons occupationally exposed to tick bite, the presence of antibodies against SFG rickettsiae was found in 54 (36.0% of the total). In the control group, the frequency of positive findings was only 4.7%, being significantly smaller compared to the exposed group (p=0.0001). Within the exposed group, the percentage of positive results in forestry workers (50.7%) was greater than in agricultural workers (21.3%); the difference was statistically significant (p=0.0002). Also within this group, the frequency of positive findings in males(46.5%) was significantly greater than in females (21.9%) (p=0.0029). In the exposed group, the positive results tended to increase with the age of the examined persons. However, a significant relationship between age and positive findings was found only in forestry workers (c2=14.207, p=0.00264), but not in agricultural workers and total exposed workers. The frequencies of positive results in forestry workers varied significantly depending on place of work (c2=11.271, p=0.00357). Similarly, the difference between the positive reactions in agricultural workers living in 2 villages proved to be significant (34.2% vs. 8.1%; p=0.0074). The obtained results indicate that people occupationally exposed to tick bite and living in the area of eastern Poland where over half of Dermacentor reticulatus ticks harbour SFG rickettsiae, are under significantly increased risk of infection with these rickettsiae.
Źródło:
Annals of Agricultural and Environmental Medicine; 2013, 20, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Seroepidemiological study of Lyme borreliosis among forestry workers in Southern Poland
Autorzy:
Buczek, A
Rudek, A.
Bartosik, K.
Szymanska, J.
Wojcik-Fatla, A.
Powiązania:
https://bibliotekanauki.pl/articles/49580.pdf
Data publikacji:
2009
Wydawca:
Instytut Medycyny Wsi
Tematy:
Lyme borreliosis
borreliosis
serology
ELISA test
forestry
worker
Polska
forestry worker
tick-borne disease
diagnosis
antibody
treatment
Borrelia burgdorferi
tick
immunoglobulin
Źródło:
Annals of Agricultural and Environmental Medicine; 2009, 16, 2; 257-261
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Toxoplasma gondii infection in selected species of free-living animals in Poland
Autorzy:
Sroka, J.
Karamon, J.
Wójcik-Fatla, A.
Dutkiewicz, J.
Bilska-Zając, E.
Zając, V.
Piotrowska, W.
Cencek, T.
Powiązania:
https://bibliotekanauki.pl/articles/2085130.pdf
Data publikacji:
2019
Wydawca:
Instytut Medycyny Wsi
Tematy:
Polska
Toxoplasma gondii
nested PCR
genotyping
free-living animals
Opis:
Introduction and objective. Free-living animals can play an important role as a reservoir of Toxoplasma gondi;, however, data concerning this issue in Poland are still limited.The aim of study was to assess the occurrence of T. gondii infection by using molecular methods in free-living animals in selected regions of Poland. Materials and method. Tissues samples of 396 animals (foxes, muskrats, birds, martens, badgers, polecats, raccoons, minks, raccoon dogs, otters, small rodents and insectivores, and grass snakes were collected from various regions of Poland. After samples digestion, DNA was isolated using QIAmp DNA Mini Kit (Qiagen). DNA extraction from small rodents and insectivores samples was performed without digestion. Next, nested PCR (B1 gene) and, for a part of nested PCR positive amplicons, RFLP PCR, were performed according to the method by Grigg and Boothroyd (2001). The other part of nested PCR positive DNA isolates were genotyped using 5 genetic markers: SAG1, SAG2 (5’- and 3’), SAG3, BTUB and GRA6, based on the method by Dubey et al. (2006). These PCR products were sequenced and compared with the NCBI database using Blast. Results. In total, in 50 of the 396 examined animals DNA of T. gondii was detected (12.6%). The highest percentages of positive results in PCR was obtained in martens (40.9%) and badgers (38.5%), lower in birds (27.3%) and the lowest in foxes (7.4%). The RFLP and multilocus PCR analysis showed the dominance of T. gondii clonal type II (or II/III). Conclusions. The results of this study indicate the frequent T. gondii infection among free-living animals in Poland, especially martens and badgers, which may indirectly indicate that these animals contribute to the spread of the parasite in the sylvatic environment in Poland. The genotyping analysis showed the dominance of T. gondii clonal type II (or II/III).
Źródło:
Annals of Agricultural and Environmental Medicine; 2019, 26, 4; 656-660
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 18

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