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    Wyświetlanie 1-4 z 4
    Tytuł:
    Modification of PLGA microspheres’ microstructure for application as cell carriers in modular tissue engineering
    Autorzy:
    Mielan, B.
    Krok-Borkowicz, M.
    Pielichowska, K.
    Pamuła, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/285127.pdf
    Data publikacji:
    2017
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    regenerative medicine
    cell cultures
    bottom-up
    PLGA
    emulsification
    Opis:
    Microspheres (MS) made of resorbable polymer have been proposed as a cell growth support. They may be assembled to form cell constructs or be suspended in hydrogels allowing injection into injury location. High relative surface area of MS provides more efficient cell culture environment than traditional culture on flat substrates (multiwell plates, Petri dishes). In addition, MS structure, topography and surface chemistry can be modified to promote cell adhesion and proliferation. The aim of this study was to obtain resorbable poly(L-lactide-co-glycolide) (PLGA) MS and to modify their properties by changing manufacturing conditions of the oil-in-water emulsification to better control structural and microstructural properties of MS and their biological performance. To this end, water phase was modified by addition of NaCl to change ionic strength, while oil phase by addition of polyethylene glycol (PEG). Microstructural and thermal properties were assessed. Cytocompatibility tests and cell cultures with MG-63 cells were conducted to verify potential relevance of MS as cell carriers. The results showed that it is possible to obtain cytocompatible MS by oil-in-water emulsification method and to control diameter, porosity and crystallinity of MS with the use of additives to oil and/or water phases without negative changes in MS cytocompatibility. The results prove that modification of both phases make it possible to produce MS with desired/controllable properties like surface topography, porosity and crystallinity.
    Źródło:
    Engineering of Biomaterials; 2017, 20, 140; 7-11
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Manufacturing and characterization of resorbable PLGA membranes for biomedical applications
    Autorzy:
    Krok, M.
    Ferreira, C.
    Fernandes, S.
    Kościelniak, D.
    Dobrzyński, P.
    Pamuła, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/284710.pdf
    Data publikacji:
    2011
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    PLGA
    PEG
    porous membrane
    phase-separation method
    GTR
    Opis:
    Porous poly(L-lactide-co-glycolide) (PLGA) membranes were prepared by solvent-casting/porogen leaching method. Poly(ethylene-glycol) (PEG) with two molecular weights was used as a pore former. Mechanical properties of the membranes were analyzed in tensile test. Topography, pore size and surface roughness were characterized by atomic force microscopy on both sites of the membranes. PEG leached out percentage, thickness and wettability were also measured. Osteoblast-like cells were cultured on the membranes for 24 h and 6 days, and morphology, distribution and number of adhered cells as well as secretion of proteins and nitric oxide were measured. The results show that PEG molecular weight affected size and distribution of pores on both surfaces of the membranes. It resulted also in different mechanical characteristics of the membranes. In vitro experiments show that the membranes support adhesion and growth of osteoblast-like cells suggesting their usefulness for guided tissue regeneration (GTR).
    Źródło:
    Engineering of Biomaterials; 2011, 14, 104; 8-13
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Shape memory process in resorbable polymers: effect on surface properties and cell adhesion
    Autorzy:
    Costa, A. M.
    Ferreira, A. S.
    Posadowska, U.
    Krok, M.
    Smola, A.
    Dobrzyński, P.
    Pamuła, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/286068.pdf
    Data publikacji:
    2012
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    shape memory
    PLGA
    tissue regeneration
    foil
    Opis:
    The objectives of this study were to confirm the shape memory behavior of two new bioresorbable terpolymers (L-lactide, glycolide, and trimethylene carbonate: L-PLGTMC and B-PLGTMC), to follow the influence of the shape memory process on their surface properties and to test their cytocompatibility using osteoblast-like cells. For this purpose, foils of both terpolymers were prepared. The terpolymers' ability to recover up to 92-93% of the memorized shape within 10 seconds was obtained. The influence of shape memory process on the surface properties was assessed by water contact angle (WCA) measurement and atomic force microscopy (AFM) and the results suggested that both terpolymers preserved the hydrophilicity after recovery and also that B-PLGTMC polymer was rougher than L-PLGTMC (about 9 folds more). The AFM pictures showed the presence of spherical shape hills on the B-PLGTMC foil surface which after the stretching procedure became oriented toward the direction of the applied load. The terpolymers were seeded on both sides (Top and Bottom faces) with human MG63 osteoblast-like cells. Cell viability was assessed after 1, 3 and 7 days, using MTT assay. Results revealed an increasing number of metabolically active cells with the incubation time, suggesting, together with nitric oxide (NO) level determination, the cytocompatibility of both terpolymers. Cell spreading and morphology were investigated by H&E staining and obtained results corresponded well with ones of MTT and NO.
    Źródło:
    Engineering of Biomaterials; 2012, 15, 114; 8-11
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    One step 3D printing of surface functionalized composite scaffolds for tissue engineering applications
    Autorzy:
    Kotlarz, M.
    Jordan, R.
    Wegner, E.
    Dobrzyński, P.
    Neunzehn, J.
    Lederer, A.
    Wolf-Brandstetter, C.
    Pamula, E.
    Scharnweber, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/306484.pdf
    Data publikacji:
    2018
    Wydawca:
    Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
    Tematy:
    druk 3D
    PLGA
    węglan wapnia
    właściwości powierzchniowe
    3D printing
    calcium carbonate
    amphiphilic poly(2-oxazoline)
    degradation
    surface properties
    Opis:
    A successful approach widely used in materials science to adapt approved materials to specific applications is to design their surface properties. A main challenge in this area is the development of processing routes enabling for a simple but efficient surface design of complex shaped geometries. Against this background, this work aimed at the implementation of self-assembly principles for surface functionalization of 3D-printed poly(lactic-co-glycolic acid) (PLGA)-based constructs with macro- and microporous geometries via precision extruding deposition. Methods: Three-component melts from PLGA, CaCO3 and amphiphilic polymers (poly(2-oxazoline) block copolymer) were printed and their bulk and surface properties were studied. Results: Melts with up to 30 mass % of CaCO3 could be successfully printed with homogeneously distributed mineral particles. PLGA degradation during the printing process was temperature and time dependent: the molecular weight reached 10 to 15% of the initial values after ca. 120 min of heat exposure. Filament surfaces from melts containing CaCO3 show an increasing microroughness along with increasing CaCO3 content. Surface roughness and amphiphilic polymer content improve scaffold wettability with both factors showing synergistic effects. The CaCO3 content of the melts affected the inner filament structure during in vitro degradation in PBS, resulting in a homogeneous mineral particle-associated microporosity for mineral contents of 20 mass % and above. Conclusions: These results provide novel insights into the behavior of three-component melts from PLGA, CaCO3 and amphiphilic polymers during precision extruding deposition and show for the first time that self-assembly processes can be used to tailor scaffolds surface properties under such processing conditions.
    Źródło:
    Acta of Bioengineering and Biomechanics; 2018, 20, 2; 35-45
    1509-409X
    2450-6303
    Pojawia się w:
    Acta of Bioengineering and Biomechanics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-4 z 4

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