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    Wyświetlanie 1-6 z 6
    Tytuł:
    Analysis of Slovak Spotted breed for bovine beta casein A1 variant as risk factor for human health
    Autorzy:
    Miluchová, Martina
    Gábor, Michal
    Trakovická, Anna
    Powiązania:
    https://bibliotekanauki.pl/articles/1039490.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Slovak Spotted breed
    PCR-RFLP
    beta-casein
    Opis:
    The goal of work was identification A1 variant of bovine beta casein which involves ischemic heart disease and diabetes mellitus in human. The digestion of A1beta casein can result in the production of bioactive beta casomorphin-7 (BCM-7); this is not the case with A2. This bioactive peptide has been linked to physiological traits that may elicit effects on components of the vascular and immune systems. The material involved 111 Slovak Spotted breed. Bovine genomic DNA was extracted from whole blood by using commercial kit, and used in order to estimate beta-casein genotypes by means of PCR-RFLP method. The PCR products were digested with DdeI restriction enzyme. In the population included in the study were detected all three genotypes, homozygote genotype A1A1 (14 animals), heterozygote genotype A1A2 (37 animals) and homozygote genotype A2A2 (60 animals). In the total population of cattle homozygotes A2A2-0.5405 were the most frequent, while homozygotes A1A1-0.1261 were the least frequent ones. This suggests a superiority of allele A2 (0.7072) which does not produce BCM-7, and thus is safe for human consumption. The expected homozygosity for gene CSN2 is in the population stated a slight increase in homozygosity (0.5858). This caused a slight decrease in the level of possible variability realization (41.80%), which corresponds to the effective number of alleles (1.7071).
    Źródło:
    Acta Biochimica Polonica; 2013, 60, 4; 799-801
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    PCR-RFLP detection of point mutations A2143G and A2142G in 23S rRNA gene conferring resistance to clarithromycin in Helicobacter pylori strains
    Autorzy:
    Klesiewicz, Karolina
    Nowak, Paweł
    Karczewska, Elżbieta
    Skiba, Iwona
    Wojtas-Bonior, Izabela
    Sito, Edward
    Budak, Alicja
    Powiązania:
    https://bibliotekanauki.pl/articles/1039295.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Helicobacter pylori
    clarithromycin resistance
    PCR-RFLP
    point mutations
    Opis:
    Background. The occurrence of clarithromycin resistance among Helicobacter pylori strains is a major cause of the treatment failure. Resistance to this drug is conferred by point mutations in 23S rRNA gene and the most prevalent mutations are A2143G and A2142G. The aim of the study was to evaluate the occurrence of A2143G and A2142G mutations in a group of H. pylori strains resistant to clarithromycin. Materials and Methods. The study included 21 clarithromycin-resistant H. pylori strains collected between 2006 and 2009 in southern Poland. Resistance to clarithromycin was quantitatively tested with the E-test to determine the minimal inhibitory concentration (MIC value). The point mutations of H. pylori isolates were detected by PCR followed by RFLP analysis. Results. The MIC values for clarithromycin for the analyzed strains ranged from 1.5 mg/L to 64 mg/L. Nine H. pylori strains exhibited A2143G mutation and A2142G mutation was found in 9 isolates as well. The results of RFLP analysis of 3 clarithromycin-resistant strains were negative for both mutations. The average MIC values for A2143G and A2142G mutants were 6 and 30 mg/L, respectively. Conclusions. Frequencies of A2143G and A2142G mutations were the same in all isolates tested. Strains with A2143G mutation exhibited lower MIC values than A2142G mutants. Application of PCR-RFLP method for detection of clarithromycin resistance allows for better and more efficient management of H. pylori infections.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 2; 311-315
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Detection of two major cytochrome b lineages in pike-perch, Sander lucioperca, and first data on their distribution in European populations
    Autorzy:
    Kohlmann, K.
    Louati, M.
    Kersten, P.
    Bahri-Sfar, L.
    Poulet, N.
    Ben Hassine, O. K.
    Powiązania:
    https://bibliotekanauki.pl/articles/363206.pdf
    Data publikacji:
    2013
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    cytochrome b
    PCR-RFLP
    pike perch
    Sander lucioperca
    cytochrom b
    sandacz
    Opis:
    Despite of the growing interest in pike-perch for aquaculture and its economic importance in fisheries, knowledge on the population structure and phylogeography of the species is still limited. We report the discovery of two major cytochrome b lineages and describe a simple method for their detection based on PCR amplification followed by restriction digestion with Alw26I. Screening of 708 individuals showed that haplotype A was fixed or dominating in Central and East European countries, whereas haplotype B was mainly found in several French populations and Tunisian pike-perch introduced from Europe. Sequencing the complete cytochrome b cds of 17 representative individuals revealed that haplotypes A and B differed by five substitutions but also showed further differentiation of both haplotypes due to an additional substitution in a single haplotype A and B individual from France, respectively. Five partial pike-perch cytochrome b cds available from NCBI GenBank could also clearly be assigned to one or the other of the two major lineages. Therefore, this new mtDNA marker might be considered as suitable not only for studies on population structure and phylogeography of pikeperch but also to trace its introduction history and to assess the genetic composition of aquaculture brood stocks.
    Źródło:
    Environmental Biotechnology; 2013, 9, 1; 1-5
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Lack of association between UBE2E2 gene polymorphism (rs7612463) and type 2 diabetes mellitus in a Saudi population
    Autorzy:
    Alharbi, Khalid
    Khan, Imran
    Al-Sheikh, Yazeed
    Alharbi, Fawiziah
    Alharbi, Fahad
    Al-Nbaheen, May
    Powiązania:
    https://bibliotekanauki.pl/articles/1039212.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    T2DM
    UBE2E2
    PCR-RFLP
    Saudi population
    Opis:
    The ubiquitin-conjugating enzyme E2E 2 (UBE2E2) gene plays an important role in insulin synthesis and secretion under conditions in which stress to the endoplasmic reticulum is increased in β-cells. In this case-control study, we have selected rs7612462 polymorphism within UBE2E2 gene to identify in a Saudi population the type 2 diabetes mellitus (T2DM) subjects. In total, 376 subjects with T2DM and 380 controls were enrolled in this study. We have collected 5 mL of peripheral blood from each participant for biochemical and molecular analyses. PCR-RFLP was used to generate genotypes at rs7612462 in all of the study subjects. Clinical data and anthropometric measurements of the patients were significantly different from those of the controls (p<0.05). All of the subjects used in this study were non-obese (25
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 4; 769-772
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The multiple HPV infections and their possible significance in predicting the risk of the cervical cancer
    Autorzy:
    Kożańska, Aleksandra
    Pisarska, Justyna
    Baldy-Chudzik, Katarzyna
    Powiązania:
    https://bibliotekanauki.pl/articles/1070878.pdf
    Data publikacji:
    2019
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    HPV co-infection
    PCR-RFLP
    Real-Time PCR
    cervical cancer
    human papillomavirus
    Opis:
    Infections with human papillomavirus (HPV) are detected frequently. The occurrence of persistent infection with oncogenic HPV type associates with the development of cervical cancer. However, the part of HPV infections consist of the multiple viral types. The influence of HPV co-infections on the risk of the development of cervical cancer is not clear, but the occurrence of more than one viral type seems to increase the risk of advanced lesions of the uterine cervix. Additionally, possible interactions (both positive and negative) between particular HPV types in co-infections may influence the risk of the lesions progression. Although, the issue of HPV co-infections is not well known, the previous scientific reports make that the introduction of the molecular methods, allowing the identification not only single but also multiple HPV infections, in routine diagnostics seem to be important. Here, we present the PCR-RFLP and Real-Time PCR methods as the useful tools in the diagnostics of HPV infections.
    Źródło:
    World Scientific News; 2019, 123; 192-207
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Metody oparte o amplifikację DNA techniką PCR wykorzystywane w ocenie bioróżnorodności mikroorganizmów glebowych
    Methods based on DNA PCR-amplification for evaluation of the soil microbial diversity
    Autorzy:
    Łyszcz, Małgorzata
    Gałązka, Anna
    Powiązania:
    https://bibliotekanauki.pl/articles/1034148.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Przyrodników im. Kopernika
    Tematy:
    soil microorganism
    genetic diversity
    molecular markers
    PCR based methods
    ARDRA
    PCR-RFLP
    RAPD
    REP-PCR
    TRFLP
    Opis:
    Mikroorganizmy glebowe, pod względem cech genomowych i fenotypowych, stanowią wysoce zróżnicowaną grupę organizmów żywych. Z powodu tak dużej różnorodności ważne jest dobranie odpowiednich metod, dających największy stopień różnicowania mikroorganizmów. Narzędziami umożliwiającym analizę zmienności genetycznej mikroorganizmów są techniki genetyczne, a wśród nich jedną z najważniejszych jest łańcuchowa reakcja polimerazy, czyli PCR (Polymerase Chain Reaction), technika opracowana w latach 1980. Niniejsza praca stanowi przegląd podstawowych zagadnień dotyczących badania zmienności genetycznej mikroorganizmów glebowych w oparciu o markery molekularne z wykorzystaniem technik bazujących na reakcji PCR tj. PCR-RFLP, TRFLP, ARDRA, RAPD.
    Soil microorganisms represent a highly diverse group of living organisms in terms of genomic and phenotypic characteristics. Due to such a large diversity, it is important to select appropriate identification methods which would secure its most complete determination. Genetic techniques are proper tools of choice for analyzing genetic variability of microorganism, the most important of which is the polymerase chain reaction (PCR), developed in the 1980s. This work presents an overview of the basic issues concerning studies on genetic variability of soil microorganisms with help of molecular markers and application of PCR techniques such as PCR-RFLP, TRFLP, ARDRA, RAPD.
    Źródło:
    Kosmos; 2017, 66, 2; 193-206
    0023-4249
    Pojawia się w:
    Kosmos
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-6 z 6

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