- Tytuł:
- Rhizobium strains differ considerably in outer membrane permeability and polymyxin B resistance
- Autorzy:
-
Komaniecka, Iwona
Zamłyńska, Katarzyna
Zan, Radosław
Staszczak, Magdalena
Pawelec, Jarosław
Seta, Irena
Choma, Adam - Powiązania:
- https://bibliotekanauki.pl/articles/1038773.pdf
- Data publikacji:
- 2016
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
rhizobium
outer membrane
lipopolysaccharide
N-phenyl-1-naphthylamine
NPN
polymyxin B - Opis:
- Six rhizobium (Rhizobium leguminosarum bv. Trifolii TA1, Sinorhizobium meliloti 1021, Mesorhizobium huakuii IFO 15243T, Ochrobactrum lupini LUP 21T, Bradyrhizobium japonicum USDA110 and B. elkanii USDA 76) and two Escherichia coli strains (E. coli ATCC 25922 and E. coli HB 101) were compared in respect to polymyxin B and EDTA resistance, as well as bacterial outer membrane (OM) permeability to a fluorescent hydrophobic agent (N-phenyl-1-naphthylamine - NPN). TEM (Transmission Electron Microscopy) and a microbial test demonstrated that all the rhizobia were much more resistant to polymyxin B in comparison with E. coli strains. EDTA and polymyxin B enhance permeability of B. japonicum and O. lupini OM. Other rhizobia incorporated NPN independently of the presence of membrane-deteriorating agents; however, the level of fluorescence (measured as NPN absorption) was strain dependent.
- Źródło:
-
Acta Biochimica Polonica; 2016, 63, 3; 517-525
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł