- Tytuł:
- IGF-I and IGF-binding proteins in synovial fluid of patients with Lyme arthritis
- Autorzy:
-
Bączyk, J.
Guszczyn, T.
Romanowicz, L.
Pancewicz, S.
Moniuszko, A.
Popko, J. - Powiązania:
- https://bibliotekanauki.pl/articles/1917714.pdf
- Data publikacji:
- 2016
- Wydawca:
- Uniwersytet Medyczny w Białymstoku
- Tematy:
-
Insulin-like Growth Factor-I
Insulinlike Growth Factor-Binding Protein
metalloproteinase
synovial fluid
Lyme arthritis
post-traumatic damage - Opis:
- Purpose: Multiple cellular functions are stimulated by a Insulin-like Growth Factor-I (IGF-I). The biological activity of IGF-I is modulated by IGFbinding proteins (IGF-BPs) and at the same time, the availability of IGF-BPs may be regulated by the proteolytic activity of some metalloproteinases (MMPs). The aim of the present study was to compare the amounts of IGF-I and IGF-BPs in relation to the activity of MMP-9 in serum and knee synovial fluid from patients with Lyme arthritis (LA) and post-traumatic damage (PTD). Material and methods: Serum and synovial fluids were taken from knee joints of patients with PTD and LA. ELISA (for IGF-I assay), polyacrylamine gel electrophoresis following Western immuneblotting (for IGF-I and IGF-BPs expression), and zymography (for metalloproteinases detection), were used. Results: The concentration of IGF-I in serum and synovial fluid from LA patients were significantly lowered in comparison to PTD patients. Interesting, the synovial fluid /serum ratio of IGF-I concentrations was also lower in LA patients. Low expression IGF-BP3 and high activity of MMP-9 were detected in the LA synovial fluid. Conclusions: The high proteolytic activity of MMP- 9 results in a cleavage of both IGF-I and IGF-BP3 causing a decrease in content of these substances in LA synovial fluid. In addition, the reduction in IGF and IGF-BP amounts may affect the repair processes in joint tissues of LA patients. The low concentration of IGF-I and IGF-BP3 slows down the repair processes in the joint tissues of LA patients.
- Źródło:
-
Progress in Health Sciences; 2016, 6(1); 49-56
2083-1617 - Pojawia się w:
- Progress in Health Sciences
- Dostawca treści:
- Biblioteka Nauki
Artykuł