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Wyświetlanie 1-4 z 4
Tytuł:
Molecular characterization and seroprevalence of Echinococcus granulosus in wild boars (Sus scrofa) in south-western Iran
Autorzy:
Sarkari, B.
Mansouri, M.
Khabisi, S.A.
Mowlavi, G.
Powiązania:
https://bibliotekanauki.pl/articles/5608.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
molecular characteristics
seroprevalence
Echinococcus granulosus
wild boar
genotype
Sus scrofa
Iran
Opis:
This study presents the first molecular and serological evaluation of Echinococcus granulosus infections in wild boars in Iran. Twenty five wild boars were collected in south-western Iran, during authorized hunting program, from March to October 2013, necropsied and examined for E. granulosus infection. Furthermore, seroprevalence of cystic echinococcosis in hunted boars was evaluated by an ELISA system. A fertile hydatid cyst due to E. granulosus was detected in the lung of one of the animals. Genotype analysis of the isolate was determined by analyzing a mitochondrial gene, cytochrome C oxidase subunit 1 (co1). DNA was extracted from the cyst sample and polymerase chain reaction amplification and DNA sequencing of the specific region of the co1 gene was performed. Molecular evaluation confirmed the presence of a sheep strain, the G1 genotype, in the wild boar in south-western Iran. This is the first report of the presence of G1 genotype of E. granulosus in wild boar in Iran. Serological evaluation of hydatid cyst by antigen-B ELISA revealed E. granulosus antibodies in 5 (20%) of 25 wild boars. A statistically significant difference was observed between the prevalence of E. granulosus antibodies and gender while the difference between the seroprevalence of E. granulosus and age was insignificant. Findings of this study might have important implications for the prevention and control of cystic echinococcosis.
Źródło:
Annals of Parasitology; 2015, 61, 4
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Detection and molecular characterization of the Iris severe mosaic virus-Ir isolate from Iran
Autorzy:
Nateqi, M.
Habibi, M.K.
Dizadji, A.
Parizad, S.
Powiązania:
https://bibliotekanauki.pl/articles/66139.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
detection
molecular characteristics
iris severe mosaic virus
isolate
phylogenesis
iris
Iridaceae
ornamental plant
Iran
Opis:
Iris belongs to the Iridaceae family. It is one of the most important pharmaceutical and ornamental plants in the world. To assess the potyvirus incidence in natural resources of iris plants in Iran, Antigen Coated-Plate ELISA (ACP-ELISA) was performed on 490 symptomatic rhizomatous iris leaf samples, which detected the potyvirus in 36.7% of the samples. Genomic 3’ end of one mechanically non-transmitted potyvirus isolate, comprising a 3’ untranslated region (390 bp) and C-terminus of the coat protein (CP) gene (459 bp), was amplified by reverse transcription polymerase chain reaction (RT-PCR), which was ligated into pTG19-T vector. The nucleotide sequence of amplicons was compared with related sequences, using Blastn software available at NCBI GenBank, and showed the highest similarity with Iris severe mosaic virus (ISMV) isolates. The nucleotide and deduced amino acid sequence of the CP C-terminus region was more than 83% identical with other ISMV isolates, therefore this isolate was designated as ISMV-Ir. This new ISMV isolate is closely related to the Chinese ISMV-PHz in phylogenetic analysis, based on the partial nucleotide and deduced amino acid sequence of the CP region. This is the first report of ISMV occurrence on Iris spp. in Iran.
Źródło:
Journal of Plant Protection Research; 2015, 55, 3
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular characterization of the first internal transcribed spacer of rDNA of Parabronema skrjabini for the first time in sheep
Autorzy:
Hasheminasab, S.S.
Powiązania:
https://bibliotekanauki.pl/articles/6198.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
molecular characteristics
nematode
rDNA
Parabronema skrjabini
food-borne parasite
parasitic disease
sheep
polymerase chain reaction
Iran
Opis:
Parabronema skrjabini is a spirurid nematode of the family Habronematidae that lives in the abomasum of ruminants such as sheep and goats. The purpose of this study was to investigate the molecular aspects of Parabronema skrjabini in sheep. The worms were collected from sheep in Sanandaj (west of Iran). The first internal transcribed spacer (ITS) of ribosomal DNA (rDNA) nucleotide fragments of Parabronema skrjabini were amplified by polymerase chain reaction (PCR) using two pairs of specific primers (Para-Ir-R and Para-Ir-F). ITS1 homology in the sequence of this study was 69% compared with the sequence data in GenBank. To our knowledge, this is the first study in the world exploring the genetic diversity of P. skrjabini in sheep based on ITS1.
Źródło:
Annals of Parasitology; 2015, 61, 4
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Phenotypic and molecular characterization of the causal agent of chafer beetle mortality in the wheat fields of the Kurdistan province, Iran
Autorzy:
Karimi, K.
Arzanlou, M.
Ahari, A.B.
Ghazi, M.M.
Powiązania:
https://bibliotekanauki.pl/articles/65470.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
phenotypic characteristics
molecular characteristics
causal agent
chafer beetle zob.scarab beetle
Anisoplia austriaca
scarab beetle
mortality
wheat field
Kurdistan region
Iran
Opis:
We report the first case of chafer beetle [Anisoplia austriaca (Herbst 1783)] mortality caused by Actinomucor elegans var. elegans in wheat fields of the Kurdistan province, Iran. For three years, dead larvae of Anisoplia austriaca were collected from wheat fields of the Kurdistan province. Similar isolates of a fast-growing fungus were recovered from all samples. The fungal isolates were identified as A. elegans var. elegans based on morphological and cultural characteristics. The identity of the species was further confirmed using sequence data of the ITS (Internal Transcribed Spacer) region of ribosomal DNA. Koch’s postulates were fulfilled by the inoculation of the larvae of A. austriaca and Galleria mellonella (Linnaeus, 1758) (as the model insect) using the spore suspension of A. elegans var. elegans. The viability of sporangiospores was evaluated using a spore dilution technique on germination medium. The results on the pathogenicity (100% mortality in A. austriaca larvae) and viability tests (germination: 95.45%) demonstrated that A. elegans var. elegans can be considered as a potential biocontrol agent against the chafer beetle. Field experiments are still required to evaluate the capacity of A. elegans as a biological control agent.
Źródło:
Journal of Plant Protection Research; 2015, 55, 3
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-4 z 4

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