- Tytuł:
- Hsa-miR-331-3p inhibits VHL expression by directly targeting its mRNA 3-UTR in HCC cell lines
- Autorzy:
-
Cao, Yiyi
Zhang, Jinnan
Xiong, Dongmei
Wang, Dan
Wu, Ting
Huang, Ailong
Tang, Hua - Powiązania:
- https://bibliotekanauki.pl/articles/1039137.pdf
- Data publikacji:
- 2015
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
HCC
HBV
miRNA
Hsa-miR-331-3p and VHL - Opis:
- Dysregulation of miRNA is widely involved in human cancers, including hepatocellular carcinoma (HCC). Array data for miRNAs indicated that miR-331-3p might be one of the disorderly expressed miRNAs in HCC cell lines, but the function of miR-331-3p in HCC remains unclear. In this study, quantitative real time polymerase chain reaction (qRT-PCR) results indicated that miR-331-3p was up-regulated in HepG2.2.15 cells, Ad-HBV-HepG2 cells and pCH9/3091transfected SMMC7721 cells compared with their control group, respectively. miRNA target prediction software was used, and VHL was found to be one of the target genes of miR-331-3p. qRT-PCR and western blot analysis indicated VHL expression was decreased when miR-331-3p was over-expressed and increased when miR-331-3p was inhibited in SMMC7721 cells. The luciferase reporter activity was inhibited in SMMC7721 cells when co-transfected with miR-331-3p expression vector and VHL 3'-UTR wild type vector and increased in HepG2.2.15 transfected with miR-331-3p inhibitor compared to its control group respectively. When co-transfected with miR-331-3p expression vector and VHL 3'-UTR mutated type vector in SMMC7721 cells the luciferase reporter activity was recovered. All of these results show that HBV up-regulated miR-331-3p expression in HCC cell lines and miR-331-3p could inhibit VHL expression by directly targeting its 3'-UTR. This provided useful information in exploring the mechanism of HCC induced by HBV infection.
- Źródło:
-
Acta Biochimica Polonica; 2015, 62, 1; 77-82
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł