- Tytuł:
- The involvement of protein kinase A in the immune response of Galleria mellonella larvae to bacteria
- Autorzy:
-
Cytryńska, Małgorzata
Zdybicka-Barabas, Agnieszka
Jakubowicz, Teresa - Powiązania:
- https://bibliotekanauki.pl/articles/1041132.pdf
- Data publikacji:
- 2007
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
antibacterial activity
protein kinase A
Rp-8-Br-cAMPS
lysozyme
Galleria mellonella - Opis:
- The role of protein kinase A (PKA) in the humoral immune response of the greater wax moth Galleria mellonella larvae to live Gram-positive bacteria Micrococcus lysodeikticus and Gram-negative bacteria Escherichia coli was investigated. The immune challenge of larvae with both kinds of bacteria caused an increase in fat body PKA activity depending on the injected bacteria. Gram-positive M. lysodeikticus was a much better inducer of the enzyme activity than Gram-negative E. coli. The PKA activity was increased about 2.5-fold and 1.5-fold, after M. lysodeikticus and E. coli injection, respectively. The in vivo inhibition of the enzyme activity by a cell permeable selective PKA inhibitor, Rp-8-Br-cAMPS, was correlated with considerable changes of fat body lysozyme content and hemolymph antimicrobial activity in bacteria-challenged insects. The kinetics of changes were different and dependent on the bacteria used for the immune challenge of G. mellonella larvae.
- Źródło:
-
Acta Biochimica Polonica; 2007, 54, 1; 167-174
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł