- Tytuł:
- Expression of soluble recombinant TGF-β type II receptor fused with the Fc portion of human IgG1 (sTβRII-Fc) in NS0 cells
- Autorzy:
-
Kwiatkowska, Eliza
Kazimierczak, Urszula
Mackiewicz, Andrzej
Kowalczyk, Dariusz - Powiązania:
- https://bibliotekanauki.pl/articles/1041250.pdf
- Data publikacji:
- 2006
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
GS-NS0 expression system
chimeric receptor
TGF-β
TGF-βreceptor
TGF antagonist - Opis:
- We have constructed and expressed recombinant chimeric soluble TGF-β type II receptor fused with the Fc portion of human IgG1 (sTβRII-Fc) in NS0 mouse myeloma cells and isolated cell lines constitutively secreting very high levels of biologically active protein. The GS-NS0 expression system takes advantage of the strong human cytomegalovirus immediate early promoter expression vector and glutamine synthetase as a selectable marker. The recombinant chimeric receptor could be produced in high amounts and efficiently purified by one step chromatography on a protein A column. Biochemical studies revealed that recombinant sTβRII-Fc binds native TGF-β1 and TGF-β3 isoforms and neutralizes their activity in vitro.
- Źródło:
-
Acta Biochimica Polonica; 2006, 53, 2; 361-369
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł