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Wyświetlanie 1-3 z 3
Tytuł:
Screening for Potato Resistance to Blackleg and Soft Rot
Autorzy:
Lebecka, Renata
Powiązania:
https://bibliotekanauki.pl/articles/2199696.pdf
Data publikacji:
2017-06-20
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
Dickeya
laboratory test
pectinolytic bacteria
Pectobacterium
Opis:
Two diseases of the potato, blackleg of potato plants and soft rot of tubers, are caused by several species of pectinolytic bacteria which belong to two genera: Pectobacterium and Dickeya. Resistance to these bacteria is polygenic and the expression of resistance in tubers and plants is only partially related, as well as strongly dependent on the aggressiveness of the bacteria and on environmental factors. Two methods of assessing tuber and stem tissue resistance of potato cultivars and breeding lines are described.
Źródło:
Plant Breeding and Seed Science; 2017, 75; 97-104
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isolation, Identification and Preservation of Pectinolytic Bacteria Pathogenic to Potato
Autorzy:
Lebecka, Renata
Powiązania:
https://bibliotekanauki.pl/articles/2199695.pdf
Data publikacji:
2017-06-20
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
Blackleg
Dickeya
Pectobacterium
Polymerase Chain Reaction
selective medium
soft rot
Opis:
Blackleg of potato plants and soft rot of tubers are caused by several species of pectinolytic bacte-ria from genera Pectobacterium and Dickeya. The text describes simple methods of isolating bacteria from symptomatic and symptomless organs of potato plants, their identification using Polymerase Chain Reaction (PCR) and preservation.
Źródło:
Plant Breeding and Seed Science; 2017, 75; 87-96
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular characterization and pathogenicity of Erwinia spp. associated with pineapple [Ananas comosus (L.) Merr.] and papaya (Carica papaya L.)
Autorzy:
Ramachandran, K.
Manaf, U.A.
Zakaria, L.
Powiązania:
https://bibliotekanauki.pl/articles/66802.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
molecular characteristics
pathogenicity
Erwinia
pineapple
Ananas comosus
papaya
Carica papaya
Dickeya
Opis:
The Erwinia species are well-known pathogens of economic importance in Malaysia causing serious damage to high-value fruit crops that include pineapple [Ananas comosus (L.) Merr.] and papaya (Carica papaya L.).The 16S rRNA sequence using eubacteria fD1 and rP2 primers, identified two bacteria species; Dickeya zeae from pineapple heart rot, and Erwinia mallotivora from papaya dieback. Phylogenetic analysis based on the neighbor-joining method indicated that all the bacterial isolates clustered in their own taxa and formed monophyletic clades. From the pathogenicity test, all isolates of D. zeae and E. mallotivora showed pathogenic reactions on their respective host plants. Genetic variability of these isolates was assessed using repetitive sequence-based PCR (rep-PCR) fingerprinting. The results indicated interspecies, and intraspecies variation in both species’ isolates. There were more polymorphic bands shown by rep-PCR fingerprints than enterobacterial repetitive intergenic consensus (ERIC) and BOX- PCRs, however both species’ isolates produced distinguishable banding patterns. Unweighted pair-group method with arithmetic averages (UPGMA) cluster analysis indicated that all Dickeya and Erwinia isolates from the same species were grouped in the same main cluster. Similarity among the isolates ranged from 77 to 99%. Sequencing of 16S rRNA using eubacteria fD1 and rP2 primers, and rep-PCR fingerprinting revealed diversity among Dickeya and Erwinia isolates. But this method appears to be reliable for discriminating isolates from pineapple heart rot and papaya dieback.
Źródło:
Journal of Plant Protection Research; 2015, 55, 4
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-3 z 3

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