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Wyświetlanie 1-15 z 25
Tytuł:
Mammalian DNA methyltransferases
Autorzy:
Siedlecki, Pawel
Zielenkiewicz, Piotr
Powiązania:
https://bibliotekanauki.pl/articles/1041231.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
DNA methyltransferases
DNA methylation
Opis:
DNA methylation is an epigenetic process affecting gene expression and chromatin organization. It can heritably silence or activate transcription of genes without any change in their nucleotide sequences, and for a long time was not recognized as an important regulatory mechanism. However, during the recent years it has been shown that improper methylation, especially hypermethylation of promoter regions, is observed in nearly all steps of tumorigenesis. Aberrant methylation is also the cause of several major pathologies including developmental disorders involving chromosome instabilities and mental retardation. A great progress has been made in our understanding of the enzymatic machinery involved in establishing and maintaining methylation patterns. This allowed for the development of new diagnostic tools and epigenetic treatment therapies. The new approaches hold a great potential; several inhibitors of DNA methyltransferases have already shown very promising therapeutic effects.
Źródło:
Acta Biochimica Polonica; 2006, 53, 2; 245-256
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
DNA methylation dynamics under drought stress in barley
Autorzy:
Chwialkowska, K.
Szarejko, I.
Kwasniewski, M.
Powiązania:
https://bibliotekanauki.pl/articles/81198.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
DNA methylation
gene expression
Hordeum vulgare
drought stress
spring barley
cytosine methylation
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Olsalazine inhibits cell proliferation and DNA methylation in canine lymphoid tumor cell lines
Autorzy:
Itoh, S.
Yamazaki, J.
Iwahana, M.
Tsukamoto, A.
Powiązania:
https://bibliotekanauki.pl/articles/2087141.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
DNA methylation
lymphoid tumors
olsalazine
canines
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 4; 515-523
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Exogenous steroid hormones stimulate full development of autonomous endosperm in Arabidopsis thaliana
Autorzy:
Rojek, J.
Pawelko, L.
Kapusta, M.
Naczk, A.
Bohdanowicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/59176.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
steroid hormone
endosperm
Arabidopsis thaliana
apomixis
DNA methylation
Opis:
Most flowering plants, including important crops, require double fertilization to form an embryo and endosperm, which nourishes it. Independence from fertilization is a feature of apomictic plants that produce seeds, from which the plants that are clones of the mother plant arise. The phenomenon of apomixis occurs in some sexual plants under specific circumstances. Since the launch of a fertilization-independent mechanism is considered a useful tool for plant breeding, there have been efforts to artificially induce apomixis. We have been able to produce fertilization-independent endosperm in vitro in Arabidopsis over the last few years. This paper demonstrates the methods of improving the quality of the endosperm obtained using plant and mammalian steroid hormones. Additionally, it shows the study on the autonomous endosperm (AE) formation mechanism in vitro. This paper examines the effect of exogenous steroid hormones on unfertilized egg and central cell divisions in culture of unpollinated pistils of Arabidopsis Col-0 wild-type andfie-1 mutant. All media with hormones used (estrone, androsterone, progesterone, and epibrassinolide) stimulated central cell divisions and fertilization-independent endosperm development. The stages of AE development followed the pattern of Arabidopsis thaliana wild type after fertilization. Subsequent stages of AE were observed from 2-nuclear up to cellular with the most advanced occurring on medium with 24-epibrassinolide and progesterone. The significant influence of mammalian sex hormones on speed of AE development and differentiation was noticed. Using restriction analysis, the changes in methylation of FIE gene was established under in vitro condition. The authors of this paper showed that Arabidopsis thaliana has a high potency to fertilization-independent development.
Źródło:
Acta Societatis Botanicorum Poloniae; 2015, 84, 2
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Changes of DNA methylation and hydroxymethylation in plant protoplast cultures
Autorzy:
Moricová, Pavla
Ondřej, Vladan
Navrátilová, Božena
Luhová, Lenka
Powiązania:
https://bibliotekanauki.pl/articles/1039600.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Brassica oleracea
DNA hydroxymethylation
Cucumis sativus
protoplasts
DNA methylation
Opis:
Cytosine methylation patterns in higher eukaryotes are important in gene regulation. Along with 5-methylcytosine (5-mC), a newly discovered constituent of mammalian DNA, 5-hydroxymethylcytosine (5-hmC), is the other modified base in higher organisms. In this study we detected 5-hmC in plant protoplast DNA and demonstrated its increasing content during the first 72 hrs. of protoplast cultivation. In contrast to 5-hmC, the amount of 5-mC decreased during protoplast cultivation. It was also found that 5-hmC did not primarily arise as a product of oxidative DNA damage following protoplast culture.
Źródło:
Acta Biochimica Polonica; 2013, 60, 1; 33-36
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Link between methyl nutrients and the DNA methylation process in the course of selected diseases in adults
Autorzy:
Lobos, P.
Regulska-Ilow, B.
Powiązania:
https://bibliotekanauki.pl/articles/2086571.pdf
Data publikacji:
2021
Wydawca:
Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
Tematy:
DNA methylation
diet
methyl nutrients
one-carbon metabolism
epigenetics
Opis:
DNA methylation is a reversible epigenetic modification that plays a crucial role in transcriptional gene silencing. Both excessive (hypermethylation) and reduced DNA methylation (hypomethylation) can contribute to the disturbance of the proper course of many important processes in the human body. The aim of the study was to discuss the relationship between methyl nutrients and the DNA methylation process in the course of selected diseases in adults. Methyl nutrients include folates (vitamin B9), riboflavin (vitamin B2), cobalamin (vitamin B12), pyridoxine (vitamin B6) and choline (vitamin B4), as well as methionine and betaine. These substances play the role of both substrates and cofactors in transformations related to one-carbon metabolism. The deficiency of methyl nutrients in the body can lead to disturbances in SAM synthesis, which is the primary donor of methyl groups in the DNA methylation process. However, the mechanism explaining the discussed relationship has not been fully explained so far. Both the concentration in the body and the intake of folate and vitamin B12 in the diet can, to some extent, have an effect on the level of DNA methylation in healthy people. In comparison, data on the effect of excessive intake of vitamin B12 in the diet on the risk of cancer development are inconsistent. An adequate betaine and choline intake in the diet might not only affect the overall improvement of the DNA methylation profile, but, to some extent, also reduce the risk of cancer, the effect of which can depend on the content of folic acid in the body. Research results on the effect of supplementation of methyl nutrients on the DNA methylation process are inconclusive. It is therefore necessary to conduct further research in this area to draw clear conclusions
Źródło:
Roczniki Państwowego Zakładu Higieny; 2021, 72, 2; 123-136
0035-7715
Pojawia się w:
Roczniki Państwowego Zakładu Higieny
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Methylenetetrahydrofolate reductase gene polymorphisms in Egyptian Turner Syndrome patients
Autorzy:
Ismail, Manal
Zarouk, Waheba
Ruby, Mona
Mahmoud, Wael
Gad, Randa
Powiązania:
https://bibliotekanauki.pl/articles/1038999.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
chromosomal nondisjunction
DNA methylation
folate
MTHFR gene
Turner Syndrome
Opis:
Background: Folate metabolism dysfunctions can result in DNA hypomethylation and abnormal chromosome segregation. Two common polymorphisms of the methylenetetrahydrofolate reductase (MTHFR) encoding gene (C677T and A1298C) reduce MTHFR activity, but when associated with aneuploidy, the results are conflicting. Turner Syndrome (TS) is an interesting model for investigating the association between MTHFR gene polymorphisms and nondisjunction because of the high frequency of chromosomal mosaicism in this syndrome. Objective: To investigate the association of MTHFR gene C677T and A1298C polymorphisms in TS patients and their mothers and to correlate these polymorphisms with maternal risk of TS offspring. Subjects and Methods: MTHFR C677T and A1298C polymorphisms were genotyped in 33 TS patients, their mothers and 15 healthy females with their mothers as controls using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and sequencing technique. Results: Genotype and allele frequencies of both C677T and A1298C were not significantly different between TS cases and controls. There were no significant differences in C677T genotype distribution between the TS mothers and controls (p=1). The MTHFR 1298AA and 1298AC genotypes were significantly increased in TS mothers Vs. control mothers (p=0.002). The C allele frequency of the A1298C polymorphism was significantly different between the TS mothers and controls (p=0.02). The association of A1298C gene polymorphism in TS patients was found to increase with increasing age of both mothers (p=0.026) and fathers (p=0.044) of TS cases. Conclusion: Our findings suggest a strong association between maternal MTHFR A1298C and risk of TS in Egypt.
Źródło:
Acta Biochimica Polonica; 2015, 62, 3; 529-532
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparative analysis of CpG islands in two genotypes of African swine fever virus
Autorzy:
Yu, Y.-Y.
X, M.-S.
Liu, Q.
Powiązania:
https://bibliotekanauki.pl/articles/16539329.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
African Swine Fever Virus (ASFV)
genotype
DNA methylation
CpG island
Opis:
African swine fever (ASF) is an acute, hemorrhagic, and devastating viral infectious disease that causes important economic losses to the swine industry. Currently, there are no effective vaccines or drugs available. Epigenetic mechanisms, especially cytosine methylation of cytosine-phosphate-guanine (CpG) islands, have a significant impact on the life cycle of several viruses. Hence, drugs targeting DNA methylation may potentially be used for the treatment of ASF. Here, we selected the inner core, core shell, inner membrane, capsid, and external envelope membrane, to analyze the characteristics of CpG islands in the ASF virus (ASFV) genomes. Furthermore, we analyzed the promoters and CpG islands in the upstream regions of these genes. Results showed that the CpG islands of seven genes were conserved in the genomes of two genotype of ASFV strains, whereas the CpG islands of other genes were relatively conserved (ASFV strains differed mainly in the quantity of CpG islands). The different distribution of CpG islands in the genomes of different ASFV strains may affect their methylation status, which may in turn affect the regulation of viral gene expression, leading to different clinical outcomes. In addition, the predicted promoter regions based on the upstream sequences of most genes overlapped with CpG island positions. Methylation of the binding sites of the promoter regions inhibits the binding of the transcription factors to the promoters, thus inhibiting the activation of the promoters and limiting the synthesis of viral proteins. The results of this study provide a basis for exploring new antiviral therapeutic strategies from an epigenetic perspective.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 3; 455-462
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Correlation of chromosome damage and promoter methylation status of the DNA repair genes MGMT and hMLH1 in Chinese vinyl chloride monomer (VCM)-exposed workers
Autorzy:
Wu, Fen
Liu, Jing
Qiu, Yu-Lan
Wang, Wei
Zhu, Shou-Min
Sun, Pin
Miao, Wen-Bin
Li, Yong-Liang
Brandt-Rauf, Paul W.
Xia, Zhao-Lin
Powiązania:
https://bibliotekanauki.pl/articles/2179802.pdf
Data publikacji:
2013-03-01
Wydawca:
Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Tematy:
vinyl chloride monomer
chromosome damage
MGMT
hMLH1
DNA methylation
Opis:
Objective: To explore the association of the methylation status of MGMT and hMLH1 with chromosome damage induced by vinyl chloride monomer (VCM). Materials and Methods: Methylation of MGMT and hMLH1 was measured in 101 VCM-exposed workers by methylation-specifi c PCR. Chromosome damage in peripheral blood lymphocytes was measured by the cytokinesis-block micronucleus assay. The subjects were divided into chromosome damaged and non-damaged groups based on the normal reference value of micronuclei frequencies determined for two control groups. Results: MGMT promoter methylation was detectable in 5 out of 49 chromosome damaged subjects, but not in the chromosome non-damaged subjects; there was a signifi cant difference in MGMT methylation between the two groups (p < 0.05). Conclusions: We detected aberrant promoter methylation of MGMT in a small number of chromosome damaged VCM-exposed workers, but not in the chromosome non-damaged subjects. This preliminary observation warrants further investigation in a larger study.
Źródło:
International Journal of Occupational Medicine and Environmental Health; 2013, 26, 1; 173-182
1232-1087
1896-494X
Pojawia się w:
International Journal of Occupational Medicine and Environmental Health
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Paidagogos (παιδαγωγός) i ars educandi (αρς εδυκανδι) w kontekście epigenetyki
Paidagogos (παιδαγωγός) and ars educandi (αρς εδυκανδι) in the context of epigenetics
Autorzy:
Dyk, Wiesław
Powiązania:
https://bibliotekanauki.pl/articles/2011184.pdf
Data publikacji:
2020
Wydawca:
Uniwersytet Szczeciński. Wydawnictwo Naukowe Uniwersytetu Szczecińskiego
Tematy:
epigenetics
nutrigenomics
nutrigenetics
DNA methylation
parenting
epigenetyka
nutrigenomika
nutrigenetyka
metylacja
wychowanie
Opis:
Ewolucyjnie każdy żywy organizm związany jest ze swym naturalnym środowiskiem. Dostosowanie się do otoczenia i jego zmian, by przeżyć, zawsze towarzyszyło człowiekowi. Dbałość o pokarm, ale nie przejadanie się, było przepustką do obecnego i przyszłego życia. Przeżyły te gatunki, które dostosowały pokarm do potrzeb swojego organizmu. Wśród Homo sapiens przeżył gatunek Homo sapiens sapiens, kromaniończyk, gdyż dzięki wysokiej inteligencji dbał o jakość pożywienia. Współczesne technologie żywności i pęd życia wprowa¬dził degradację ludzkiej natury. Powstała niezgodność między genami i pożywieniem. Nie zawsze wyżywienie służy organizmowi człowieka i nie zawsze umożliwia jego osobowościowe, kreatywne trwanie w środowisku naturalnym. Aby działać i tworzyć najpierw trzeba się właściwie odżywiać. Kształtowanie osobowości wsparte jest rodzajem, jakością i wartością pożywienia.
From evolution standpoint, every living organism is bound with its environment. Adaptation to the environment and its changes had always accompanied Man. Taking care of food but not overeating was a pass to current and future life. The species which did not overeat, had survived. Amongst Homo sapiens, Homo sapiens sapiens and Homo Cro-Magnon survived, because having future in mind, it took care of the food’s quality. Contemporary food technologies and rate of living introduced degeneration of human nature. A chaos had occurred between genes and food. Food is not always in benefit of human organism and its personal, creative existence in natural environment. To act and create you must be first full. Shaping the personality is supported by the type, quality and value of food.
Źródło:
Studia Koszalińsko-Kołobrzeskie; 2020, 27; 359-370
1230-0780
2719-4337
Pojawia się w:
Studia Koszalińsko-Kołobrzeskie
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Epi-genes potentiate plant biodiversity
Autorzy:
Szopa, J.
Powiązania:
https://bibliotekanauki.pl/articles/951285.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
biodiversity
oligonucleotide
DNA methylation
gene expression
protein
methylase
polymerase
RNA polymerase
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Changes in DNA methylation in maize under herbicide stress conditions
Autorzy:
Tyczewska, A.
Gracz, J.
Szymkowiak, J.
Twardowski, T.
Powiązania:
https://bibliotekanauki.pl/articles/951318.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
DNA methylation
chromatin modification
gene silencing
gene transcription
maize
herbicide stress
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Epigenetics is promising direction in modern science
Autorzy:
Fartushok, Tetiana
Kovalyshyn, Orysia
Fedevych, Yuri
Tanchyn, Igor
Zhykovskiy, Volodymyr
Powiązania:
https://bibliotekanauki.pl/articles/2175208.pdf
Data publikacji:
2021
Wydawca:
Towarzystwo Chemii i Inżynierii Ekologicznej
Tematy:
epigenetics
5-methylcytosine
DNA methylation
histone
epigenetyka
5-metylocytozyna
metylacja DNA
histon
Opis:
Epigenetics studies the inherited changes in a phenotype or in expression of genes caused by other mechanisms, without changing the nucleotide sequence of DNA. The most distinguished epigenetic tools are: modifications of histones, enzymatic DNA methylation, and gene silencing mediated by small RNAs (miRNA, siRNA). The resulting m5C residues in DNA substantially affect the cooperation of proteins with DNA. It is organized by hormones and aging-related alterations, one of the mechanisms controlling sex and cellular differentiation. DNA methylation regulates all genetic functions: repair, recombination, DNA replication, as well as transcription. Distortions in DNA methylation and other epigenetic signals lead to diabetes, premature aging, mental dysfunctions, and cancer.
Źródło:
Chemistry-Didactics-Ecology-Metrology; 2021, 26, 1-2; 123--135
2084-4506
Pojawia się w:
Chemistry-Didactics-Ecology-Metrology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A comparison of the in vitro genotoxicity of anticancer drugs idarubicin and mitoxantrone.
Autorzy:
Błasiak, Janusz
Gloc, Ewa
Warszawski, Mariusz
Powiązania:
https://bibliotekanauki.pl/articles/1043821.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
mitoxantrone
oxidative DNA damage
DNA damage
idarubicin
comet assay
DNA methylation
DNA repair
Opis:
Idarubicin is an anthracycline antibiotic used in cancer therapy. Mitoxantrone is an anthracycline analog with presumed better antineoplastic activity and lesser toxicity. Using the alkaline comet assay we showed that the drugs at 0.01-10 μM induced DNA damage in normal human lymphocytes. The effect induced by idarubicin was more pronounced than by mitoxantrone (P < 0.001). The cells treated with mitoxantrone at 1 μM were able to repair damage to their DNA within a 30-min incubation, whereas the lymphocytes exposed to idarubicin needed 180 min. Since anthracyclines are known to produce free radicals, we checked whether reactive oxygen species might be involved in the observed DNA damage. Catalase, an enzyme inactivating hydrogen peroxide, decreased the extent of DNA damage induced by idarubicin, but did not affect the extent evoked by mitoxantrone. Lymphocytes exposed to the drugs and treated with endonuclease III or formamidopyrimidine-DNA glycosylase (Fpg), enzymes recognizing and nicking oxidized bases, displayed a higher level of DNA damage than the untreated ones. 3-Methyladenine-DNA glycosylase II (AlkA), an enzyme recognizing and nicking mainly methylated bases in DNA, increased the extent of DNA damage caused by idarubicin, but not that induced by mitoxantrone. Our results indicate that the induction of secondary malignancies should be taken into account as side effects of the two drugs. Direct strand breaks, oxidation and methylation of the DNA bases can underlie the DNA-damaging effect of idarubicin, whereas mitoxantrone can induce strand breaks and modification of the bases, including oxidation. The observed in normal lymphocytes much lesser genotoxicity of mitoxantrone compared to idarubicin should be taken into account in planning chemotherapeutic strategies.
Źródło:
Acta Biochimica Polonica; 2002, 49, 1; 145-155
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Quantification of 5-methyl-2-deoxycytidine in the DNA
Autorzy:
Giel-Pietraszuk, Małgorzata
Insińska-Rak, Małgorzata
Golczak, Anna
Sikorski, Marek
Barciszewska, Mirosława
Barciszewski, Jan
Powiązania:
https://bibliotekanauki.pl/articles/1039105.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
DNA methylation
3,N4-etheno-5-methyl-2'deoxcytidine
fluorescence
RP-HPLC
Opis:
Methylation at position 5 of cytosine (Cyt) at the CpG sequences leading to formation of 5-methyl-cytosine (m5Cyt) is an important element of epigenetic regulation of gene expression. Modification of the normal methylation pattern, unique to each organism, leads to the development of pathological processes and diseases, including cancer. Therefore, quantification of the DNA methylation and analysis of changes in the methylation pattern is very important from a practical point of view and can be used for diagnostic purposes, as well as monitoring of the treatment progress. In this paper we present a new method for quantification of 5-methyl-2'deoxycytidine (m5C) in the DNA. The technique is based on conversion of m5C into fluorescent 3,N4-etheno-5-methyl-2'deoxycytidine (εm5C) and its identification by reversed-phase high-performance liquid chromatography (RP-HPLC). The assay was used to evaluate m5C concentration in DNA of calf thymus and peripheral blood of cows bred under different conditions. This approach can be applied for measuring of 5-methylcytosine in cellular DNA from different cells and tissues.
Źródło:
Acta Biochimica Polonica; 2015, 62, 2; 281-286
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
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