- Tytuł:
- Laccase concentration by foam fractionation of Cerrena unicolor and Pleurotus sapidus culture supernatants
- Autorzy:
-
Blatkiewicz, M.
Antecka, A.
Górak, A.
Ledakowicz, S. - Powiązania:
- https://bibliotekanauki.pl/articles/185371.pdf
- Data publikacji:
- 2017
- Wydawca:
- Polska Akademia Nauk. Czytelnia Czasopism PAN
- Tematy:
-
foam fractionation
Laccase
CTAB
Polysorbate 80
frakcjonowanie pianą
Polisorbat 80 - Opis:
- Foam fractionation process for concentration of laccases from two Basidiomycete strains under different process conditions was investigated. Culture supernatants of Cerrena unicolor and Pleurotus sapidus containing active laccase were used with and without surfactant additives. Two surfactants: cationic cetrimonium bromide (CTAB) and non-ionic Polysorbate 80 were applied in the range from 0.2 mM to 1.5 mM. The pH levels ranging from 3 to 10 were examined with particular attention to pH=4, which is close to the pI of the enzymes. Results show that the source of the enzyme is significant in terms of partitioning efficiency in a foam fractionation process. Laccase from Cerrena unicolor showed the best activity partitioning coefficients between foamate and retentate of almost 200 with yields reaching 50% for pH 7.5 and concentration of CTAB cCTAB = 0.5 mM, whereas laccase from Pleurotus sapidus showed partitioning coefficients of up to 8 with 25% yield for pH 4 and cCTAB = 0.5 mM.
- Źródło:
-
Chemical and Process Engineering; 2017, 38, 3; 455-464
0208-6425
2300-1925 - Pojawia się w:
- Chemical and Process Engineering
- Dostawca treści:
- Biblioteka Nauki
Artykuł