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Wyszukujesz frazę "CRISPR/Cas" wg kryterium: Temat


Wyświetlanie 1-4 z 4
Tytuł:
Plant virus resistance biotechnological approaches : From genes to the CRISPR/Cas gene editing system
Autorzy:
Iksat, Nurgul
Masalimov, Zhaksylyk
Omarov, Rustem
Powiązania:
https://bibliotekanauki.pl/articles/27312654.pdf
Data publikacji:
2023
Wydawca:
Instytut Technologiczno-Przyrodniczy
Tematy:
CRISPR/Cas9
CRISPR/Cas13
plant disease resistance
plant immunity
plant virus
Opis:
Plant viruses cause crop losses in agronomically and economically important crops, making global food security a challenge. Although traditional plant breeding has been effective in controlling plant viral diseases, it is unlikely to solve the problems associated with the frequent emergence of new and more virulent virus species or strains. As a result, there is an urgent need to develop alternative virus control strategies that can be used to more easily contain viral diseases. A better understanding of plant defence mechanisms will open up new avenues for research into plant-pathogen interactions and the development of broad-spectrum virus resistance. T he scientific literature was evaluated and structured in this review, and the results of the reliability of the methods of analysis used were filtered. As a result, we described the molecular mechanisms by which viruses interact with host plant cells. To develop an effective strategy for the control of plant pathogens with a significant intensity on the agricultural market, clear and standardised recommendations are required. The current review will provide key insights into the molecular underpinnings underlying the coordination of plant disease resistance, such as main classes of resistance genes, RNA interference, and the RNA-mediated adaptive immune system of bacteria and archaea - clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated Cas proteins - CRISPR/Cas. Future issues related to resistance to plant viral diseases will largely depend on integrated research to transfer fundamental knowledge to applied problems, bridging the gap between laboratory and field work.
Źródło:
Journal of Water and Land Development; 2023, 57; 147--158
1429-7426
2083-4535
Pojawia się w:
Journal of Water and Land Development
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
CRISPR/Cas9 in plant biotechnology: applications and challenges
Autorzy:
Gan, W.C.
Ling, A.P.K.
Powiązania:
https://bibliotekanauki.pl/articles/2096248.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
CRISPR/Cas9
crop improvement
gene editing
plant biotechnology
regulations
Opis:
The application of plant biotechnology to enhance beneficial traits in crops is now indispensable because of food insecurity due to increasing global population and climate change. The recent biotechnological development of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated system 9 (Cas9) allows for a more simple and precise method of gene editing, which is now preferred compared to Zinc Finger Nucleases (ZFNs) and Transcription Activator-like Effector Nucleases (TALENs). In this review, recent progress in utilizing CRISPR/Cas9-mediated gene editing in plants to enhance certain traits in beneficial crops, including rice, soybean, and oilseed rape, is discussed. In addition, novel methods of applying the CRISPR/Cas9 system in live cell imaging are also extensively reviewed. Despite all the applications, the existing delivery methods of CRISPR/Cas9 fail to provide consistent results and are inefficient for in planta transformation. Hence, research should be focused on improving current delivery methods or developing novel ones to facilitate CRISPR/Cas9-based gene editing studies. Strict regulations on the sale and commercial growth of gene-edited crops have restricted more efforts in applying CRISPR/Cas9 technology in plant species. Therefore, a shift in public viewpoint toward gene editing would help to propel scientific progress rapidly.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2022, 103, 1; 81-93
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Synthetic chromosome - the first element for synthetic life
Autorzy:
Noszka, Mateusz
Kilisch, Julia
Powiązania:
https://bibliotekanauki.pl/articles/1165623.pdf
Data publikacji:
2018
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
CRISPR/Cas9
DNA assembly
synthetic biology
synthetic chromosome
λ Red
Opis:
Synthetic biology is a scientific area, that link together domains such as: biotechnology, microbiology, system biology, genetic engineering and bioinformatics. The main goal of synthetic biology is to constructing and designing artificial biological systems or re-design of existing biological systems for useful purposes - synthesizing for knowledge and synthesizing for products. The main structure on which synthetic biologists focus is the chromosome. Thanks to the development of modern genome editing techniques such as CRISPR/Cas9, or DNA assembly methods in recent years, there are more and more possibilities of creating synthetic chromosomes. The current achievements give hope for the construction of a fully synthetic organism into the future, as well as open up new possibilities related to the synthesis of new products. The following paper presents actual knowledge about synthetic chromosome including the topic of genome editing method and potential applications of synthetic biology.
Źródło:
World Scientific News; 2018, 107; 185-195
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Pharmacological versus genetic inhibition of heme oxygenase-1 - the comparison of metalloporphyrins, shRNA and CRISPR/Cas9 system
Autorzy:
Mucha, Olga
Podkalicka, Paulina
Czarnek, Maria
Biela, Anna
Mieczkowski, Mateusz
Kachamakova-Trojanowska, Neli
Stepniewski, Jacek
Jozkowicz, Alicja
Dulak, Jozef
Loboda, Agnieszka
Powiązania:
https://bibliotekanauki.pl/articles/1038402.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
CRISPR/Cas9
shRNA
inhibitors
heme oxygenase-1
HO-1
off-target
Opis:
Inhibition of heme oxygenase-1 (HO-1, encoded by HMOX1), a cytoprotective, anti-apoptotic and anti-inflammatory enzyme, may serve as a valuable therapy in various pathophysiological processes, including tumorigenesis. We compared the effect of chemical inhibitors - metalloporphyrins, with genetic tools - shRNA and CRISPR/Cas9 systems, to knock-down (KD)/knock-out (KO) HO-1 expression/activity. 293T cells were incubated with metalloporphyrins, tin and zinc protoporphyrins (SnPPIX and ZnPPIX, respectively) or were either transduced with lentiviral vectors encoding different shRNA sequences against HO-1 or were modified by CRISPR/Cas9 system targeting HMOX1. Metalloporphyrins decreased HO activity but concomitantly strongly induced HO-1 mRNA and protein in 293T cells. On the other hand, only slight basal HO-1 inhibition in shRNA KD 293T cell lines was confirmed on mRNA and protein level with no significant effect on enzyme activity. Nevertheless, silencing effect was much stronger when CRISPR/Cas9-mediated knock-out was performed. Most of the clones harboring mutations within HMOX1 locus did not express HO-1 protein and failed to increase bilirubin concentration after hemin stimulation. Furthermore, CRISPR/Cas9-mediated HO-1 depletion decreased 293T viability, growth, clonogenic potential and increased sensitivity to H2O2 treatment. In summary, we have shown that not all technologies can be used for inhibition of HO activity in vitro with the same efficiency. In our hands, the most potent and comprehensible results can be obtained using genetic tools, especially CRISPR/Cas9 approach.
Źródło:
Acta Biochimica Polonica; 2018, 65, 2; 277-286
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-4 z 4

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