- Tytuł:
- Non-histone nuclear protein HMGN2 differently regulates the urothelium barrier function by altering expression of antimicrobial peptides and tight junction protein genes in UPEC J96-infected bladder epithelial cell monolayer
- Autorzy:
-
Tian, Hanwen
Miao, Junming
Zhang, Fumei
Xiong, Feng
Zhu, Feimei
Li, Jinyu
Wang, Xiaoying
Chen, Shanzhe
Chen, Junli
Huang, Ning
Wang, Yi - Powiązania:
- https://bibliotekanauki.pl/articles/1038529.pdf
- Data publikacji:
- 2018
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
HMGN2
UPEC
BECs 5637
bladder epithelium
tight junction
antimicrobial peptides - Opis:
- The urinary tract is vulnerable to frequent challenges from environmental microflora. Uropathogenic Escherichia coli (UPEC) makes a major contribution to urinary tract infection (UTI). Previous studies have characterized positive roles of non-histone nuclear protein HMGN2 in lung epithelial innate immune response. In the study presented here, we found HMGN2 expression was up-regulated in UPEC J96-infected urothelium. Surprisingly, over-expression of HMGN2 promoted disruption of BECs 5637 cells' intercellular junctions by down-regulating tight junction (TJs) components' expression and physical structure under J96 infection. Further investigation showed that BECs 5637 monolayer, in which HMGN2 was over-expressed, had significantly increased permeability to J96. Our study systemically explored the regulatory roles of HMGN2 in BECs barrier function during UPEC infection and suggested different modulations of intracellular and paracellular routes through which UPEC invades the bladder epithelium.
- Źródło:
-
Acta Biochimica Polonica; 2018, 65, 1; 93-100
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł