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Wyszukujesz frazę "Marcelli, A." wg kryterium: Autor


Wyświetlanie 1-2 z 2
Tytuł:
Infrared Synchrotron Radiation: from Condensed Matter to Biology Researches
Autorzy:
Marcelli, A.
Iliescu, C.
Powiązania:
https://bibliotekanauki.pl/articles/2028923.pdf
Data publikacji:
2001-11
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
78.30.-j
87.64.-t
87.57.-s
41.60.Ap
87.14.Ee
Opis:
Infrared spectroscopy is probably the oldest spectroscopic method applied to investigate materials and chemico-physical phenomena. Nowadays, infrared spectroscopy represents the characterization technique most applied in the industry and in many technological processes. In the last decades a significant progress has been achieved in the use of the intense and brilliant infrared emission from electron storage rings previously used only as VUV and X-ray sources. In the infrared range the low energy of the electron beam does not affect the synchrotron radiation spectral distribution, while high current will make storage rings the most brilliant infrared sources to be used for infrared spectroscopy and micro-spectroscopy. Infrared micro-spectroscopy is a unique technique that combines microscopy and spectroscopy for purposes of micro-analysis. Spatial resolution, within a microscopic field of view, is the goal of the modern infrared micro-spectroscopy applied to condensed matter physics, materials science, biophysics, and now to medicine. Although limited in spatial resolution, infrared is able to resolve chemistry using the contrast of the absorption lines. Fourier transform-infrared micro-spectroscopy using synchrotron radiation is now able to collect data with 2-4 cm^{-1} resolution on the scale of 10-100 seconds up to an area of a few microns opening a new scenario: infrared spectroscopy of entire cells and tissue. Moreover, distributions of functional groups such as proteins, lipids, and nucleic acids can be achieved inside a single living cell with a spatial resolution of a few microns.
Źródło:
Acta Physica Polonica A; 2001, 100, 5; 647-658
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Methodology for FTIR Imaging of Individual Cells
Autorzy:
Yao, Seydou
Cestelli Guidi, M.
Delugin, M.
Della-Ventura, G.
Marcelli, A.
Petibois, C.
Powiązania:
https://bibliotekanauki.pl/articles/1398831.pdf
Data publikacji:
2016-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
82.80.-d
87.85.Pq
87.64.-t
Opis:
FTIR imaging is a novel spectroscopic technique able to provide cell imaging, in vivo and in real-time. However, one key issue is developing methodologies for cell culture on IR-transparent substrates fitting cell biology requirements. In this work we tested different IR-transparent substrates in terms of biotoxicity, surface properties, and spectral image acquisition qualities. Only a few substrates, namely Si₃N₄, Ge, GLS, LaF₃, Si, SrF₂, ZnS/C, ZnS/F, were found to provide cell culture conditions comparable to those observed on usual polycarbonate Petri dishes, the main limiting parameter being the toxicity of the material (ZnS, GLS, PbF₂, PbCl₂) or a poor adhesiveness (notably diamond, AgCl, CaF₂, ZnS). From substrates eligible for a good-quality cell culture, the spectral acquisition quality is mainly affected by the refractive index value. Finally, the best compromise between cell culture quality and image spectral quality could be obtained using Si and Ge substrates. This rationalization of the available IR-transparent substrates for bioimaging is particularly relevant for live cell analyses, where cell culture conditions must remain unaffected by substrate properties.
Źródło:
Acta Physica Polonica A; 2016, 129, 2; 250-254
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-2 z 2

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