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    Wyświetlanie 1-4 z 4
    Tytuł:
    Elemental Mapping of Prostate Tissue by Micro-SRIXE
    Autorzy:
    Banaś, A.
    Banaś, K.
    Falkenberg, G.
    Dyduch, G.
    Kwiatek, W. M.
    Powiązania:
    https://bibliotekanauki.pl/articles/2044669.pdf
    Data publikacji:
    2006-03
    Wydawca:
    Polska Akademia Nauk. Instytut Fizyki PAN
    Tematy:
    87.64.-t
    Opis:
    Synchrotron radiation-induced X-ray emission is now a proven analytical method for the determination of trace elements. Better insight of the role of trace elements in tissue samples can be gained by acquiring complete distribution maps of these elements. Our approach is based on the acquisition of digital maps. In this contribution we present two different methods for obtaining normalization matrix: first - based on argon intensity in the single spectrum and second - based on changes of beam current intensity. The region for the scan was carefully chosen according to the histological view of the sample. A total area was scanned with a step size of 15 μm in each direction and measuring time of 30 s per pixel. The thickness of the samples was 15 μm. A polycapillary was used for beam focusing. At the energy of 18 keV the beam size on the sample was approximately 15 μm which is of the order of a cell diameter.
    Źródło:
    Acta Physica Polonica A; 2006, 109, 3; 323-328
    0587-4246
    1898-794X
    Pojawia się w:
    Acta Physica Polonica A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Analysis of Human Lenses by Raman Microspectroscopy
    Autorzy:
    Paluszkiewicz, C.
    Chaniecki, P.
    Rękas, M.
    Rajchel, B.
    Piergies, N.
    Kwiatek, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/1398828.pdf
    Data publikacji:
    2016-02
    Wydawca:
    Polska Akademia Nauk. Instytut Fizyki PAN
    Tematy:
    87.64.kp
    42.55.Ye
    87.64.-t
    Opis:
    A cataract is an opacity (clouding) of the normally clear lens which develops as a result of aging, metabolic disorders, trauma or heredity. The number of patients with cataract is increasing exponentially. This disease requires surgical intervention, to remove the cloudy lens and to introduce the eye lens polymer. In this work we will present analyses of degraded parts of human lens. Experimental materials were obtained from the lens removed during surgical intervention. These biological samples were measured using Almega XR Confocal Raman spectrometer (Thermo Scientific) with an excitation source of 785 nm laser line. The Raman vibrations in the spectral region of 650-1750 cm¯¹ were analyzed. The difference spectra revealed an excess of tryptophan, tyrosine, phenylalanine, β-sheet conformation, and molecules or molecular groups.
    Źródło:
    Acta Physica Polonica A; 2016, 129, 2; 244-246
    0587-4246
    1898-794X
    Pojawia się w:
    Acta Physica Polonica A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Chemical Composition of Atherosclerotic Plaques οf apoE/LDLR-Double Knockout Mice by Synchrotron Radiation FTIR Microspectroscopy
    Autorzy:
    Kowalska, J.
    Gajda, M.
    Kwiatek, W.
    Franczyk-Żarów, M.
    Kostogrys, R.
    Chłopicki, S.
    Sandt, C.
    Dumas, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/1490259.pdf
    Data publikacji:
    2012-02
    Wydawca:
    Polska Akademia Nauk. Instytut Fizyki PAN
    Tematy:
    87.19.xr
    87.64.km
    87.64.-t
    Opis:
    Atherosclerosis is a multietiological inflammatory disease of large and medium-sized arteries of increasing incidence in westernized countries. The aim of this study was to identify the biochemical changes during the progression of atherosclerosis by synchrotron radiation Fourier transform infrared microspectroscopy in atheromas of apoE/$LDLR^{-//-}$ mice fed egg-rich diet supplemented or not with angiotensin converting enzyme inhibitor perindopril. Synchrotron radiation Fourier transform infrared microspectroscopy technique was used to obtain information at high spatial resolution about the distribution of proteins (C-N, N-H, CO for amide I and amide II bands), lipids ($CH_2$, $CH_3$ bands) as well as mineral deposits (calcium carbonates and phosphates). Total contents of lipids and proteins were found to be significantly lower in animals treated with the diet and perindopril. An increase in saturation level of lipids was observed in animals fed with egg-rich diet when compared to the normal diet and perindopril treatment, which did not inhibit this effect. Moreover, a significant change in the secondary structure of proteins (ratio between absorption bands 1634 $cm^{-1}$/1656 $cm^{-1}$ attributed to β-type and α-type, respectively) was observed in both experimental groups in comparison with the control. Principal component analysis was used to analyse the recorded spectra. It has revealed that higher content of phosphates (wavenumber range 950-1020 $cm^{-1}$) was observed between egg-rich diet fed animals and the control group.
    Źródło:
    Acta Physica Polonica A; 2012, 121, 2; 555-560
    0587-4246
    1898-794X
    Pojawia się w:
    Acta Physica Polonica A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Distinguishing Prostate Cancer from Hyperplasia
    Autorzy:
    Kwiatek, W. M.
    Banaś, A.
    Banaś, K.
    Podgórczyk, M.
    Dyduch, G.
    Falkenberg, G.
    Gajda, M.
    Cichocki, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/2044731.pdf
    Data publikacji:
    2006-03
    Wydawca:
    Polska Akademia Nauk. Instytut Fizyki PAN
    Tematy:
    87.64.-t
    Opis:
    The prostate gland is the most common site of neoplastic disorders in men. The knowledge of the pathogenesis of benign prostate hyperplasia and prostate cancer is still under investigation. X-ray fluorescence analysis using synchrotron radiation seems to be an appropriate technique to study the elemental composition of biological tissues. The measurements presented in this paper were carried out on the L-beam line at the HASYLAB, DESY (Germany). The observed concentrations of Fe, Cu, Mn, and Zn are significantly higher in cancerous tissues, as compared to normal and hyperplastic ones. Our results reveal also the existence of the two types of hyperplasia.
    Źródło:
    Acta Physica Polonica A; 2006, 109, 3; 377-381
    0587-4246
    1898-794X
    Pojawia się w:
    Acta Physica Polonica A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-4 z 4

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