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Wyszukujesz frazę "Rudnicka, K." wg kryterium: Autor

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    Wyświetlanie 1-5 z 5
    Tytuł:
    Selection and optimization of staining techniques of Chinese hamster lung fibroblast V79 cell preparations
    Autorzy:
    Rudnicka, K.
    Krzyżewski, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/363128.pdf
    Data publikacji:
    2011
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    linia komórkowa V79
    barwnik Giemsy
    Schiff
    Light Green
    cell line V79
    Giemsa
    Opis:
    We present the results of optimization of fixation and staining of the Chinese hamster lung fibroblast V79 cell line. The experiment included four methods of fixation and three methods of staining and enabled the selection of the best visualization technique for V79 cell lines culture. The Schiff method of colouring with Light Green contrasted well the cell nucleus and the cytoplasm, but the staining was light-sensitive and transient. The Giemsa staining made cell preparations suitable for microscopic evaluation, although the difference between the nucleus and the cytoplasm was not always easily recognisable.
    Źródło:
    Environmental Biotechnology; 2011, 7, 2; 84-87
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro micronucleus test assessment of polycyclic aromatic hydrocarbons
    Autorzy:
    Żelazna, K.
    Rudnicka, K.
    Tejs, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/363120.pdf
    Data publikacji:
    2011
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    test mikrojądowy in-vitro
    blok cytokinetyczny
    linia komórkowa
    WWA
    właściwości mutagenne
    in vitro MN test
    cytokinesis-block micronucleus assay
    cell line
    PAH
    mutagenic properties
    Opis:
    Polycyclic aromatic hydrocarbons (PAH) can be dangerous due to their genotoxic properties and the formation of DNA adducts in the target organs. The increasing number of environmental pollutants containing polycyclic aromatic compounds requires the use of inexpensive and relatively quick assays enabling evaluation of potential genotoxicity of chemicals. Therefore, the in vitro micronucleus assay (MN) has been developed as a rapid screening test based on measuring and counting the micronuclei in various cells subjected to the examined compound. Combined with the cytokinesis-block micronucleus (CBMN) technique and the fluorescence in situ hybridization (FISH) assay, it also enables classification of compound mutagenic activity into aneugenic or clastogenic categories, the identification of the mechanism of MNi formation and the estimation of malsegregations of chromosomes amongst the two nuclei in binucleated cells. The combination of these different approaches is very useful in performing risk assessments of PAH with threshold types of dose responses.
    Źródło:
    Environmental Biotechnology; 2011, 7, 2; 70-80
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genotoxicity of cyclopentha[c]phenanthrene and its two derivatives based on an in vitro micronucleus test
    Autorzy:
    Rudnicka, K.
    Łuczyński, M. K.
    Góra, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/363298.pdf
    Data publikacji:
    2012
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    derivatives
    genotoxicity
    in vitro micronucleus test
    PAHs
    V79
    pochodne
    genotoksyczność
    test mikrojądrowy in vitro
    Opis:
    Environmental pollution causes a variety of health problems, including cancer. Many known pollutants have carcinogenic properties and polycyclic aromatic hydrocarbons (PAH) belong to this group. In this study, an in vitro culture of V79 cells of the Chinese hamster was subjected to three tested PAHs: 5-amino-2,3-dihydro-1H-cyclopenta-[c]phenanthrene (ACP[c]Ph), 5-amino-9-methoxy-2,3-dihydro-1H-cyclopenta[c]phenanthrene (AMCP[c]Ph) and cyclopenta[c]phenanthrene (CP[c]Ph). The in vitro micronucleus (MN) assay was applied in order to evaluate the genotoxic properties of the studied compounds. The highest genotoxic effect was observed for AMCP[c]Ph in a concentration of 0.02μg·ml-1. The genotoxic effect of the other two compounds was slightly lower.
    Źródło:
    Environmental Biotechnology; 2012, 8, 2; 63-66
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Molecular modelling techniques in environmental research
    Autorzy:
    Urniaż, R. D.
    Rutkowska, E.
    Jastrzębski, J. P.
    Książek, P.
    Rudnicka, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/363208.pdf
    Data publikacji:
    2013
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    molecular dynamics
    molecular modeling
    pharmacokinetics
    pharmacophore modeling
    QSAR
    dynamika molekularna
    modelowanie molekularne
    farmakokinetyka
    modelowanie farmakoforowe
    Opis:
    Over the last few decades significant increase in computational methods (in silico) was annotated. Novel methods have been developed and applied for hypothesis improvement and testing in regions of industrial, pharmaceutical and environmental research. The term in silico methods include variety of approaches. Considerable attention has been attracted to databases, data analysis tools, quantitative structure-activity relationships (QSAR), pharmacophore models, molecular docking and dynamics, pharmacokinetics and other molecular modelling techniques. In silico methods are often accompanied by experimental data, both to create the model and to test it. Such models are frequently used in the discovery and optimization of novel molecules with expected affinity to a target, the estimation of absorption, distribution, metabolism, excretion and toxicity properties as well as physicochemical characterization. The review summarizes briefly the applications of most common molecular modelling techniques and evaluates their application in environmental research. Additionally, this study considers computer aided methods as potential and complex tools that may serve as valuable partnership with wet-lab experiments and may provide a rational aid to minimize the cost and time of research.
    Źródło:
    Environmental Biotechnology; 2013, 9, 2; 39-51
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Assessment of mutagenic activity of methyl- and phenylphenanthrenes based on Salmonella test and micronucleus test
    Autorzy:
    Rudnicka, K.
    Tejs, S.
    Budzikur, K. A.
    Mielżyńska-Švach, D.
    Jakimiuk, E.
    Chachaj, A.
    Góra, M.
    Żelazna, K.
    Łuczyński, M. K.
    Powiązania:
    https://bibliotekanauki.pl/articles/363178.pdf
    Data publikacji:
    2013
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    Ames test
    in vitro
    micronucleus test
    mutagenicity
    phenanthrene
    test Amesa
    mutagenność
    test mikrojądrowy
    fenanatren
    Opis:
    Polycyclic aromatic hydrocarbons (PAHs) are widely spread environmental pollutants mainly originating from anthropogenic sources such as fossil fuel combustion, industries, and others. Although a large body of literature exists on the toxicity and carcinogenicity of PAHs, primarily benzo[a]pyrene, toxicity data for phenanthrene deriveratives are very limited. The main aim of the experiment was to investigate if there exists correlation between molecular structure and mutagenic activity of four phenanthrene derivatives: 1 methylphenanthrene, 4 methylphenanthrene, 1 phenylphenanthrene, and 4 phenylphenanthrene. An Ames assay using two strains of histidine dependent Salmonella Typhimurium (TA98 and TA100) was conducted to assess the mutagenic activity of studied compounds both in the presence (+S9) and in the absence (-S9) of an exogenous source of metabolic activation. The compounds were also tested in an in vitro chromosome aberration assay in which V-79 cells were exposed to the phenanthrene derivatives investigated both in the presence and in the absence of metabolic activation. The phenylphenanthrenes showed no mutagenic effect. These compounds occasionally induced significant decrease in the number of revertants in the Ames test. The greatest mutagenic effects were observed for 1 methylphenanthrene after metabolic activation (+S9). In the micronucleus test the greatest mutagenic effect was observed for 4 methylphenanthrene also in the presence of metabolic activation system. The results obtained are comparable to those reported earlier for the methylphenanthrenes.
    Źródło:
    Environmental Biotechnology; 2013, 9, 2; 65-71
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-5 z 5

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