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    Wyświetlanie 1-10 z 10
    Tytuł:
    Preliminary evaluation of mutagenic activity of two amino derivatives of cyclopenta[c]phenanthrene
    Autorzy:
    Łuczyński, M. K.
    Góra, M.
    Mielżyńska, D.
    Brzuzan, P.
    Tejs, S.
    Pirogowicz, P.
    Luczynski, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/363278.pdf
    Data publikacji:
    2007
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    test Amesa
    fenantren
    mutacje DNA
    WWA
    Ames test
    phenanthrene
    DNA mutations
    PAHs
    Opis:
    The mutagenicity of two, newly synthesized amino-derivatives of cyclopenta[c]phenanthrene (CP[c]Ph) was investigated in the Ames test using TA98 and TA100 histidine dependent Salmonella typhimurium strains. Neither of the examined compounds showed mutagenic activity without metabolic activation. The incorporation of an activation system caused high mutagenicity of CP[c]Ph derivatives in both S. typhimurium strains. The results are compared with the previous data on the mutagenic activity of unsubstituted CP[c]Ph and possible mechanisms of activation are discussed.
    Źródło:
    Environmental Biotechnology; 2007, 3, 1; 15-19
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Polymorphism of microsatellite loci - a tool in studying biodiversity of paddlefish aquaculture broodstock
    Autorzy:
    Kaczmarczyk, D.
    Kohlmann, K.
    Kersten, P.
    Luczynski, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/363088.pdf
    Data publikacji:
    2007
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    izolacja DNA
    DNA mikrosatelitarne
    amplifikacja PCR
    DNA extraction
    microsatellite DNA
    PCR amplification
    Opis:
    American paddlefish (Polyodon spathula) is a new species in Polish aquaculture, its broodstocks are few and small, and it is possible that all mature fish originated from only a few spawners. Studies on polymorphism of highly variable microsatellite DNA allow revealing genetic characteristics of individual spawners as well as estimation of genetic variation within and divergence between broodstocks. This paper describes optimised protocols for isolation of DNA from fin tissues, amplification of nine microsatellite loci using PCR technique, and for fish genotyping using automatic capillary DNA sequencer. Our technique was tested towards the fin samples taken from all paddlefish reared in Poland and approaching their sexual maturity; the study included also samples taken from 47 fish of the Ukrainian breeding center (Gorny Tykich).
    Źródło:
    Environmental Biotechnology; 2007, 3, 2; 44-48
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Relative quantification of CYP1A gene expression in whitefish (Coregonus lavaretus) exposed to benzo[a]pyrene
    Autorzy:
    Brzuzan, P.
    Jurczyk, Ł.
    Łuczyński, M. K.
    Góra, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/363188.pdf
    Data publikacji:
    2005
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    ekspresja genu CYP1A
    sieja
    benzo(a)piren
    real-time PCR
    benzo(a)pyrene
    Coregonus lavaretus
    CYP1A gene expression
    RealTime PCR
    relative quantification
    Opis:
    The expression of CYP1A (cytochrome P4501A) can be induced by a number of aromatic compounds in teleost fishes. We developed a real-time PCR assay for measuring relative quantities (RQ) of CYP1A mRNA in whitefish (Coregonus lavaretus). To test for the usefulness of the assay we performed a treatment study, using benzo[a]pyrene (B[a]P) a model CYP1A inducer. Primers for the CYP1A gene were adapted from the literature, whereas those for [beta]-actin (endogenous control) were designed from a region that was found to be conserved among salmonid [beta]-actin genes. A group of hatchery raised whitefish, with an average body mass of 15 g and total length of 12 cm were given an intraperitoneal injection (10 mg/kg) of B[a]P in corn oil (2 mg B[a]P/ml corn oil) or corn oil alone (Control). After 48 h, whitefish liver, head kidney and brains were collected for mRNA isolation and analysis. In all three tissues sampled, CYP1A mRNA was affected by treatment with B[a]P. Head kidney tissue showed the greatest induction potential (RQ=11.00) from base levels (RQ=1.00), followed by liver (RQ=9.45), and brain (RQ=3.76). These results demonstrated that CYP1A was highly inducible by B[a]P in whitefish head kidney and liver, and to some extent, in brain tissue. The approach presented here has the advantage of providing rapid and accurate measures of CYP1A induction in various tissues of fish responding to PAH contaminant exposure.
    Źródło:
    Environmental Biotechnology; 2005, 1, 1; 11-15
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    CYP1A gene expression in adipose fin of rainbow trout (Oncorhynchus mykiss Walbaum) exposed to benzo[a]pyrene
    Autorzy:
    Brzuzan, P.
    Woźny, M.
    Łuczyński, M. K.
    Góra, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/363276.pdf
    Data publikacji:
    2007
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    płetwa tłuszczowa
    benzo(a)piren
    CYP1A mRNA
    skrzela
    pstrąg tęczowy
    real-time PCR
    adipose fin
    benzo(a)pyrene
    gill
    rainbow trout
    Real-Time PCR
    Opis:
    Proximate to the environment, adipose fin of fish may be considered as a lipid storing tissue, and thus can be a target for either waterborne or dietary polycyclic aromatic compounds (PACs). We determined the effects of benzo[a]pyrene (B[a]P), a model PAC member, on CYP1A gene expression in adipose fin and compared that with the effects in gill of juvenile rainbow trout (Oncorhynchus mykiss Walbaum) using the quantitative reverse transcription polymerase chain reaction (Q-RT-PCR). The results of the study demonstrated that constitutive CYP1A mRNA was present in adipose fin of rainbow trout, but the transcripts were far less abundant than those in gill tissue. We confirmed high CYP1A gene induction potential of the gills in rainbow trout injected with benzo[a]pyrene, but also showed moderately and transiently induced CYP1A mRNA in adipose fin. The modest and transitory gene expression may preclude rainbow trout adipose fin CYP1A mRNA levels from using it as an indicator of sustained exposure of fish to the polycyclic aromatic compounds.
    Źródło:
    Environmental Biotechnology; 2007, 3, 1; 20-24
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Oxidative metabolism, mutagenic and carcinogenic properties of some polycyclic aromatic hydrocarbons
    Autorzy:
    Łuczyński, M. K.
    Góra, M.
    Brzuzan, P.
    Wilamowski, J.
    Kozik, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/363172.pdf
    Data publikacji:
    2005
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    metabolizm tlenowy
    właściwości mutagenne
    właściwości rakotwórcze
    wielopierścieniowe węglowodory aromatyczne
    oxidative metabolism
    mutagenic properties
    carcinogenic properties
    polycyclic aromatic hydrocarbons
    Opis:
    This attempt to synthesize the present state of knowledge on the correlation between the structure of chosen PAHs and their mutagenic or carcinogenic activity shows that the dependencies cannot be treated univocally. Extreme complexity of the subject, typical for investigations on the boundary between chemistry and biology, inclines one to search for new ways of synthesis of new compounds, which would be of interest for biologists studying the problem of chemical carcinogenesis. On the other hand, there is a strong need for elaborating a standardized test that would explicitly define the carcinogenic properties of a specific compound. The use of a variety of methods for the determination of carcinogenic potency of chemical compounds combined with simultaneous differentiation of structure of examined compounds, sometimes leads to contradictory conclusions. Correlation between mutagenic and carcinogenic activity of some compounds is also sometimes questionable. Modern molecular biology should allow for elaborating a method which would give a satisfactory and reliable answer to the fundamental question: is a specific compound carcinogenous for a human organism? And if so - to what extent?
    Źródło:
    Environmental Biotechnology; 2005, 1, 1; 16-28
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    MicroRNA expression in liver of whitefish (Coregonus lavaretus) exposed to microcystin-LR
    Autorzy:
    Brzuzan, P.
    Woźny, M.
    Wolińska, L.
    Piasecka, A.
    Łuczyński, M. K.
    Powiązania:
    https://bibliotekanauki.pl/articles/363280.pdf
    Data publikacji:
    2010
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    Coregonus lavaretus
    Dicer
    histon H2A
    mikrocystyna LR
    miRNAs
    toksyczność
    histone H2A
    microcystin LR
    toxicity
    Opis:
    MicroRNAs (miRNAs) are small, highly conserved, non-coding RNAs that regulate gene expression of target mRNAs through cleavage or translational inhibition. In the field of toxicology, the relationship between toxicity and microRNA expression is poorly understood. In the present study we analyzed the abundance of 9 selected miRNAs (omy-miR-21, omy-miR-21t, omy-miR-122, omy-miR-125a, omy-miR-125b, omy-miR-125t, omy-miR-199-5a, omy-miR-295, omy-let-7a) and mRNA of 3 genes (histone H2A, ribosome protein rpl19, and Dicer which is a miRNA processing enzyme) in liver samples of whitefish exposed to Microcystin-LR (MC-LR) at a dose of 100µg*kg-1body weight for 24 or 48h. In the examined liver tissue, omymiR-122 showed the highest relative constitutive level, what is consistent with data obtained from fish and mammals. Unexpectedly, the reference H2A mRNA level was consistently up-regulated (over 20-fold; P<0.05) in fish liver after both 24 and 48h of exposure to MC-LR. The result may suggest that MC-LR acts as an initiator of specific cell-physiologic signals triggering DNA replication in fish liver cells. MC-LR treatment had no effect on the examined miRNAs levels, except for omy-miR-125a and omy-let-7a. Whereas omy-miR-125a was up-regulated (ER=2.68; S.E. 1.61-6.78; P<0.05), omy-let-7a was down-regulated (ER=0.55; S.E. 0.32-0.79; P<0.05) in whitefish liver after 48h of the treatment with MC-LR, when compared to controls. More work with the fish is essential for understanding the crosstalk of the regulatory network controlled by the two miRNAs in the context of MC-LR toxicity.
    Źródło:
    Environmental Biotechnology; 2010, 6, 2; 53-60
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Benzo[a]pyrene and cyclopenta[c]phenanthrene suppress expression of p53 in head kidney of rainbow trout
    Autorzy:
    Brzuzan, P.
    Woźny, M.
    Góra, M.
    Łuczyński, M. K.
    Jabłońska, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/363082.pdf
    Data publikacji:
    2011
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    B[a]P
    CP[c]Ph
    ekspresja genów
    hnRNA
    pstrąg tęczowy
    poziom transkrypcji
    gene expression
    rainbow trout
    transcription rate
    Opis:
    Although p53, a protein of important tumor suppressive function, has been extensively studied in mammals, relatively little is known about the p53 pathways in lower vertebrates. Particularly, limited information exists on possible influences of environmental contaminants on the expression of the p53 gene in fish. In the current study, we assessed the effects of benzo[a]pyrene (B[a]P; potent tumor promoter) and cyclopenta[c]phenanthrene (CP[c]Ph; clastogenic agent) exposure on a 24h profile of p53 gene expression in head kidney of juvenile rainbow trout (Oncorhynchus mykiss). To analyze the p53 transcription rate, we developed protocol for the examination of both mRNA and heterogeneous nuclear (hn) RNA of the gene, using Real-Time RTPCR approach. The results show that both compounds are capable of suppressing p53 transcriptional activity within 12h of the treatment. Our finding supports the idea that structurally different PAHs may influence cell physiologic functions controlled by p53 in fish, in part, by down-regulating its RNA expression levels.
    Źródło:
    Environmental Biotechnology; 2011, 7, 1; 41-45
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Preliminary evaluation of ER- and AhR-mediated gene expression patterns in rainbow trout (Oncorhynchus mykiss) liver after short-term exposure to zearalenone in binary mixtures
    Autorzy:
    Woźny, M.
    Brzuzan, P.
    Wolińska, L.
    Góra, M.
    Łuczyński, M. K.
    Powiązania:
    https://bibliotekanauki.pl/articles/363144.pdf
    Data publikacji:
    2010
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    receptor AhR
    benzo(a)piren
    ścieżka sygnałowa
    17beta-estradiol
    receptor estrogenowy
    zearalenone
    aryl hydrocarbon receptor
    benzo(a)pyrene
    crosstalk
    estrogen receptor
    Opis:
    While estrogenic properties of mycotoxin zearalenone (ZEA) has been an extensively studied issue, little is known about molecular background of its biological responses that cannot be simply explained by the estrogenic potential. The present study describes effects of ZEA (10mg*kg-1 body weight) in binary mixtures either with benzo[a]pyrene (B[a]P), or with 17β-estradiol (E2) on ER- and AhR-dependent gene expression in juvenile rainbow trout liver, evaluated using Real-Time qPCR. The study revealed dual nature of ZEA, as the treatment with this compound alone increased mRNA levels of both ER- and AhR-mediated gene expression. However, our results did not show any synergistic or additive effect of ZEA in binary mixures with E2 or B[a]P on studied gene expression levels. Whether the intriguing potential of ZEA to elicit distinct signals was a result of binding affinity to AhR or/and ER and AhR mutual receptor interactions, should be investigated in further experiments.
    Źródło:
    Environmental Biotechnology; 2010, 6, 1; 16-23
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Preliminary study on adverse effects of phenanthrene and its methyl and phenyl derivatives in larval zebrafish, Danio rerio
    Autorzy:
    Wolińska, L.
    Brzuzan, P.
    Woźny, M.
    Góra, M.
    Łuczyński, M. K.
    Podlasz, P.
    Kolwicz, S.
    Piasecka, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/363152.pdf
    Data publikacji:
    2011
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    toksyczność ostra
    CYP1A
    cyp1b1
    geny vtg
    ekspresja mRNA
    stężenie nie wywołujące efektu
    WWA
    Real-Time qPCR
    acute toxicity
    cyp1a genes
    cyp1b1 genes
    vtg genes
    mRNA expression
    No Effect Concentration
    PAHs
    Opis:
    Toxic effects of polycyclic aromatic hydrocarbons (PAHs) have been extensively studied in fish, although knowledge concerning biological activities of phenanthrene and its derivatives remains still incomplete. The aim of this work was to evaluate lethal and sublethal effects of benzo(a)pyrene, phenanthrene and phenanthrene derivatives (1-methylphenanthrene, 4-methylphenanthrene, 1-phenylphenanthrene and 4-phenylphenanthrene) on zebrafish (Danio rerio) larvae. We conducted acute toxicity test, using 96h static renewal exposure to a series of the PAH concentrations (0.05, 0.50, 5.00, 50.00µmol*l-1), to determine the No Effect Concentration (NEC) value for each compound examined. The mean NEC estimates obtained in the study were 5.16۪.45µmol*l-1 (B[a]P), 4.88۪.13µmol*l-1 (Ph), 40.24䔰.93µmol*l-1 (1P-Ph), 47.92ۭ.61µmol*l-1 (1M-Ph), 24.31۱.33µmol*l-1 (4P-Ph) and 3.11۫.01µmol*l-1 (4M-Ph) and suggested the following order of PAH toxicities on Danio rerio larvae: 4M-Ph>Ph˜B[a]P>4PPhP-Ph>1M-Ph. To gain insight into possible molecular mechanisms of apparent toxicity of phenanthrene derivatives on zebrafish larvae, we examined mRNA expression of cyp1a, cyp1b1, and vtg genes in the larvae exposed for 48h to a PAH concentration of 0.50µmol*l-1. Whereas the larvae exposed to each tested PAH displayed many developmental abnormalities (i.e. pericardial and yolk sac edema, dorsal curvature, or tail malformations), no significant upregulation of cyp1a and cyp1b1 mRNA was observed, except for zebrafish exposed to B[a]P. However, significant reduction of vtg mRNA was observed in the larvae exposed to phenanthrene and its 4P- derivative. The results may contribute to the development of a new knowledge about effects of structurally diverse phenanthrene derivatives on vertebrate organisms.
    Źródło:
    Environmental Biotechnology; 2011, 7, 1; 26-33
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cyclopenta[c]phenanthrene induction of CYP1A in brain of rainbow trout (Oncorhynchus mykiss)
    Autorzy:
    Brzuzan, P.
    Woźny, M.
    Łuczyński, M. K.
    Góra, M.
    Ciesielski, S.
    Kuźmiński, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/363272.pdf
    Data publikacji:
    2007
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    benzo(a)piren
    mózg
    CYP1A
    cyklopenta[c]fenantren
    absolutna kwantyfikacja mRNA
    pstrąg tęczowy
    benzo(a)pyrene
    brain
    cyclopenta[c]phenanthrene
    mRNA absolute quantification
    rainbow trout
    Opis:
    We assessed the effects of cyclopenta[c]phenanthrene (CP[c]Ph) and benzo[a]pyrene (B[a]P; positive control) on CYP1A gene expression in brain of juvenile rainbow trout (Oncorhynchus mykiss) using the quantitative reverse transcription polymerase chain reaction (Q-RT-PCR). A group of hatchery raised rainbow trout, with an average body mass of 49.4 g and total length of 15.5 cm were given an intraperitoneal injection (10 mg*kg-1) of either CP[c]Ph or B[a]P in corn oil (2 mg*mi-1 corn oil) or corn oil alone (control). After 24 and 48 h, trout brains were collected for mRNA isolation and analysis. After 24 hours of the exposure, only B[a]P-treated rainbow trout had 10-fold higher number of CYP1A transcripts (mean = 3.63*106 transcripts*µg-1 total RNA) than control fish (3.24*105 transcripts*µg-1 total RNA; Tukey test, P<0.05). After 48 hrs, significantly higher levels of CYP1A expression (Tukey test, P<0.001) were found in either CP[c]Ph- or B[a]P- induced group (1.45*106 and 6.92*106 transcriptsźµg-1 total RNA, respectively) over a control group (mean=1.41*105 transcripts*µg-1 total RNA). The finding that CYP1A in brain tissue was inducible by CP[c]Ph, a polycyclic aromatic hydrocarbon (PAH) of different than B[a]P planar characteristics, may further validate the use of rainbow trout brain CYP1A mRNA levels as a biomarker of PAH exposure.
    Źródło:
    Environmental Biotechnology; 2007, 3, 1; 10-14
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-10 z 10

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