Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

English (EN)·Polski (PL)·Yкраїнський (UK)
Przejdź do strony domowej biblioteki Centralna Biblioteka Wojskowa Link otwiera się w nowym oknie Logo biblioteki Prolib Integro - strona głównaCentralna Biblioteka Wojskowa

Wyszukujesz frazę "marker" wg kryterium: Temat

  Lista filtrów
Wyrównaj
    Wyświetlanie 1-13 z 13
    Tytuł:
    Evaluation of genetic variability within sweet cherry (Prunus avium L.) genetic resources by molecular SSR marker
    Autorzy:
    Patrzak, J.
    Henychová, A.
    Paprštein, F.
    Sedlák, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/12687772.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    Czech Republic
    plant cultivation
    sweet cherry
    Prunus avium
    fruit tree
    genetic variability
    genetic evaluation
    genetic resource
    molecular marker
    SSR molecular marker
    EST-SSR marker
    Opis:
    Sweet cherry is a vegetatively propagated, perennial plant with high level of heterozygosity and ancient breeding history. Therefore, it is necessary to keep, conserve and evaluate known genetic resources for future breeding programs and fruit production stability. In present, the utilization of DNA molecular genetic analyses is the best suitable method for evaluation of individual accessions, thus we eliminated duplications and characterized the genetic relationships. In our work, we used PCR primer combinations for 19 SSR and 2 EST-SSR loci for analyses of 123 current, old and local sweet cherry cultivars from Czech genetic resources of Research and Breeding Institute of Pomology in Holovousy. In total, 115 polymorphic fragments were amplified, which we used for hierarchical cluster analysis of genetic variability. The result dendrograms were divided into three main clusters and ten subgroups. Clustering corresponded to genealogical and geobotanical characteristics of individual accessions as breeding history of several known accessions.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 3; 157-165
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genetic diversity of Brassica rapa germplasm of Khyber Pakhtunkhwa Pakistan revealed by molecular markers
    Autorzy:
    Ali, N.
    Ali, S.
    Khan, N.U.
    Jan, S.A.
    Rabbani, M.A.
    Hussain, I.
    Powiązania:
    https://bibliotekanauki.pl/articles/12690092.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    Pakistan
    plant breeding
    Brassica rapa
    germplasm
    genetic diversity
    plant genotype
    molecular marker
    SSR marker
    Opis:
    A total of 96 indigenous Brassica rapa accessions were collected from different locations of Khyber Pakhtunkhwa, Pakistan. Simple Sequence Repeats (SSR) markers were used to identify the most diverse genotypes among the collected lots. Twenty six (26) different SSR primers were used for (genetic) variability among collected genotypes. These primers were selected from literature based on their previous results. These primers produced 135 scorable bands of which 75 were polymorphic, with an average of 55.5% polymorphic loci, and reflected the broader genetic background of the collected genotypes. An average 2.88 polymorphic bands with an average PIC value of 0.49 was recorded. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) divided all genotypes into three main groups. Group one contained three clusters, while group two and three had four and two clusters each. Based on the UPGMA dendrogram, genotypes collected from Kohat, Bannu, Swat and Haripur showed considerable amount of variation. From the present study, it is concluded that SSR markers can be proved as the best tool for the genetic variability of other local and exotic B. rapa genotypes.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 6; 57-65
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The relationship between RAPD marker-by-marker interactions and quantitative traits of caraway (Carum carvi L.)
    Autorzy:
    Bocianowski, J.
    Seidler-Łożykowska, K.
    Nowosad, K.
    Kuczyńska, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/12681131.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    plant cultivation
    caraway
    Carum carvi
    herbal plant
    spice plant
    quantitative trait
    molecular marker
    RAPD marker
    RAPD-PCR reaction
    Opis:
    Application of molecular markers makes the selection process much more effective. Marker assisted selection is an important tool for plant breeders to increase the efficiency of a breeding process, especially for multigenic traits, highly influenced by the environment. Epistasis is the interaction between alleles from two or more loci determining the complex traits, and thus plays an important role in the development of quantitative traits of crops. In this paper, the relationships between RAPD marker-by-marker interactions and 22 quantitative traits of caraway (Carum carvi L.) were analyzed. Significant associations of 116 epistatic markers with at least one trait in 2004 as well as 112 in 2005 were found on the basis of multivariate regression analysis. The proportion of total phenotypic variances of individual trait explained by the marker-by-marker interactions ranged from 25.3% to 96.0%.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 3; 53-69
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Identification of new Polish lines of Chenopodium quinoa (Willd.) by spectral analysis of pigments and a confirmation of genetic stability with SCoT and RAPD markers
    Autorzy:
    Lema-Rumińska, J.
    Miler, N.
    Gęsiński, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/11887402.pdf
    Data publikacji:
    2018
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    plant breeding
    quinoa
    Chenopodium quinoa
    Faro cultivar
    Titicaca cultivar
    morphological feature
    spectral analysis
    pigment
    genetic stability
    RAPD marker
    SCoT marker
    Opis:
    Identification of cultivars is essential both in breeding and to settle cultivar disputes. The purpose of the study has been to examine cultivar identities based on absorption spectra of plant pigments and to confirm a genetic stability with SCoT and RAPD molecular markers in new Polish lines of Chenopodium quinoa Willd. Spectral analysis of pigments extracted from plant inflorescences in quinoa gives an opportunity to confirm the cultivar identity and identification of ‘Faro’ and ‘Titicaca’ cultivars and their new lines. Spectral analysis is an effective method of confirming cultivar identity and it should be used in practice for the identification of cultivars or cultivars lines in Chenopodium quinoa Willd. Analysis of molecular markers indicated by RAPD as well as SCoT technique revealed a high genetic stability of the derivative lines of ‘Faro’ and ‘Titicaca’, while variation was detected in plants representing original cultivars: banding pattern different than predominant was present in three plants of ‘Titicaca’ (genetic distnaces from 7.5% to 55.9%) and in a single plant of ‘Faro’(genetic distance 61.2% as indicated by SCoT technique).
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2018, 17, 1; 75-86
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Size polymorphism survey of nucleolar organizer regions (NORs) in Hampshire boars
    Ocena polimorfizmu wielkości regionów jąderkotwórczych (NOR) u knurów rasy hampshire
    Autorzy:
    Danielak-Czech, B.
    Babicz, M.
    Kozubska-Sobocińska, A.
    Rejduch, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/2197003.pdf
    Data publikacji:
    2013
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    polymorphism
    nucleolar organizer region
    Hampshire breed
    boar
    chromosome marker
    FISH technique
    Źródło:
    Annales Universitatis Mariae Curie-Skłodowska. Sectio EE: Zootechnica; 2013, 31, 4; 8-13
    0239-4243
    2083-7399
    Pojawia się w:
    Annales Universitatis Mariae Curie-Skłodowska. Sectio EE: Zootechnica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Evaluation of changes in the genetic structure of Hucul horses based on the analysis of I type of genetic markers
    Ocena zmian w strukturze genetycznej koni huculskich na podstawie analizy markerów genetycznych klasy I
    Autorzy:
    Nogaj, A.
    Nogaj, J.
    Kozubska-Sobocińska, A.
    Rychlik, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/2196899.pdf
    Data publikacji:
    2013
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    genetic structure
    structural change
    Hucul breed
    horse
    genetic marker
    antigen
    polymorphic protein
    Źródło:
    Annales Universitatis Mariae Curie-Skłodowska. Sectio EE: Zootechnica; 2013, 31, 2; 39-48
    0239-4243
    2083-7399
    Pojawia się w:
    Annales Universitatis Mariae Curie-Skłodowska. Sectio EE: Zootechnica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Identification of genetic diversity among Arnica montana L. genotypes using RAPD markers
    Analiza zróżnicowania genetycznego wśród genotypów Arnica montana L. za pomocą markerów RAPD
    Autorzy:
    Okoń, S.
    Paczos-Grzęda, E.
    Łoboda, M.
    Sugier, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/11542962.pdf
    Data publikacji:
    2014
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    DNA polymorphism
    identification
    genetic diversity
    Arnica montana
    genotype
    RAPD marker
    medicinal plant
    molecular analysis
    Opis:
    Arnica montana L. is one of the most important herbal plants used in medicine, pharmaceutical and cosmetic industry. The number of studies performed with molecular markers on arnica genotypes is very limited. Because of this fact the aims of presented examination were optimization of protocols DNA isolation from fresh leaves of A. montana and identification of genetic diversity among this plant genotypes. In presented study to obtain pure DNA Plant & Fungi DNA Purification Kit (EURx) were used. To clean obtained DNA long and slow electrophoresis and isolation DNA from gels were used. A. montana genotypes were analyzed using 40 RAPD primers (Operon Technologies), out of which 12 produced high number of polymorphic and repeatable fragments. In total, selected primers produced 120 fragments, among them 111 (92.5%) were polymorphic. The genetic similarity matrices were produced based on RAPD using the Dice’s coefficient. RAPD based genetic similarity was estimated between 0.535 and 0.945. The highest genetic similarity was estimated among GA17 and GA18 genotypes, which are closely located on the obtained dendrogramme.
    Arnica montana L. jest jedną z najcenniejszych roślin zielarskich wykorzystywanych w medycynie, farmacji i przemyśle kosmetycznym. W dostępnej literaturze liczba doniesień związanych z analizą molekularną arniki jest znikoma, dlatego też celem prezentowanych badań była optymalizacja procesu izolacji DNA ze świeżych liści oraz identyfikacja zróżnicowania genetycznego oparta na markerach RAPD. W prezentowanej pracy w celu uzyskania czystego DNA do izolacji wykorzystano zestaw DNA Plant & Fungi DNA Purification Kit (Euro) oraz oczyszczanie za pomocą długiej elektroforezy w żelu agarozowym. Spośród testowanych 40 starterów RPAD do analiz wybrano 12 generujących stabilne i polimorficzne wzory prążków. Wyselekcjonowane startery amplifikowały 120 fragmentów, spośród których 111 (92,5%) było polimorficznych. Wykorzystujac markery RAPD utworzono matryce podobieństwa genetycznego. średnia wartość podobieństwa analizowanych genotypów wynosiła 0.886. Najwyższy współczynnik podobieństwa genetycznego oszacowano pomiędzy genotypami GA17 i GA18, które ulokowały się blisko siebie na uzyskanym dendrogramie.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2014, 13, 4; 63-71
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A fast and effective protocol for obtaining genetically diverse stevia (Stevia rebaudiana Bertoni) regenerants through indirect organogenesis
    Szybki i efektywny protokół otrzymywania zróżnicowanych genetycznie regenerantów stewii (Stevia rebaudiana Bertoni) drogą pośredniej organogenezy
    Autorzy:
    Dyduch-Siemińska, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/13925501.pdf
    Data publikacji:
    2021
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    stevia
    Stevia rebaudiana
    micropropagation
    molecular marker
    random amplified polymorphic DNA
    shoot regeneration
    somaclonal variation
    Źródło:
    Agronomy Science; 2021, 76, 4; 47-62
    2544-4476
    2544-798X
    Pojawia się w:
    Agronomy Science
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Transferrin genotypes as genetic markers of lifetime prolificacy of ewes in a flock of prolific sheep 09
    Genotypy transferyny jako genetyczne markery calozyciowej plennosci maciorek w stadzie owcy plennej 09
    Autorzy:
    Steppa, R.
    Ślósarz, P.
    Strojna, A.
    Stanisz, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/2197639.pdf
    Data publikacji:
    2007
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    animal breeding
    sheep
    prolific breed
    prolificacy
    genetic marker
    transferrin
    genotype
    life time
    ewe
    Źródło:
    Annales Universitatis Mariae Curie-Skłodowska. Sectio EE: Zootechnica; 2007, 25, 1; 55-62
    0239-4243
    2083-7399
    Pojawia się w:
    Annales Universitatis Mariae Curie-Skłodowska. Sectio EE: Zootechnica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Analysis of genetic diversity of Ficus carica L. (Moraceae) collection using simple sequence repeat (SSR) markers
    Autorzy:
    Marcotuli, I.
    Mazzeo, A.
    Nigro, D.
    Giove, L.
    Giancaspro, A.
    Colasuonno, P.
    Prgomet, Ž.
    Prgomet, I.
    Tarantino, A.
    Ferrara, G.
    Gadaleta, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/12664546.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    Moraceae
    common fig
    Ficus carica
    population structure
    genetic analysis
    genetic diversity
    SSR marker
    Opis:
    Modern technologies and accurate information on genetic diversity and structure are contributing to improve the plant breeding, in particular for all the minor species with a lack of data. Genetic diversity of 139 different Ficus carica L. genotypes collected from Italy and Croatia, and divided into two subgroups: uniferous (only main crop) and biferous (breba and main crop), was investigated using 49 microsatellite markers. A total of 70 alleles were generated, of which 64 (91.4%) showed a polymorphic pattern indicating high level of genetic diversity within the studied collection. The mean heterozygosity over the 64 single locus microsatellites was 0.33 and the expected and observed averaged variance were 16.50 and 184.08, respectively. The 139 fig genotypes formed two clusters in the PCoA analysis, suggesting a division between Italian and Croatian genotypes. Moreover, the fig accessions could be divided into two main clusters based on the STRUCTURE analysis according to the biological type, uniferous or biferous, with partly overlapping varieties. In conclusion, our results demonstrated that molecular markers were able to discriminate among genotypes and useful for the authentication of fig tree varieties (homonymies and synonymies).
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 4; 93-109
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Simple verification of in vitro - grown clones of the genus Drosera L. using its molecular markers
    Autorzy:
    Fratrikova, M.
    Bauer, M.
    Jopcik, M.
    Libantova, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/11891526.pdf
    Data publikacji:
    2018
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    Drosera
    Drosera binata
    Drosera rotundifolia
    Drosera spatulata
    molecular marker
    in vitro growth
    nucleotide sequence
    molecular identification
    Opis:
    Drosera L. is a genus of carnivorous plants that comprises approximately 250 species, although this number is probably not complete. Some of these taxa exhibit only small differences in morphological traits that can be partly influenced if the taxa are propagated in vitro. Here, we focus on the verification of putative clones of Drosera spathulata Labill., Drosera rotundifolia L. and Drosera binata var. Dichotoma species cultivated in vitro using molecular markers covering the internal transcribed spacer (ITS) region of 45S ribosomal DNA (rDNA). Following the polymerase chain reaction (PCR) amplification of ~360-bp DNA fragments and sequencing, the sequences were aligned with corresponding sequences in the National Center for Biotechnology Information (NCBI) database. In addition, each of tested PCR amplicons had a specific restriction profile that predominantly enables the differentiation of D. rotundifolia and D. spathulata; the shape of the leaves does not have to be a clear morphologically distinguishable trait.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2018, 17, 1; 159-164
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Assessment of genetic diversity and relationships among grapevine cultivars originating in Central and Eastern Europe and North America using ISSR markers
    Autorzy:
    Lisek, A.
    Lisek, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/12313802.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    Central Europe
    Eastern Europe
    North America
    plant cultivation
    grapevine
    Vitis vinifera
    genetic diversity
    ISSR marker
    Opis:
    The study shows genetic diversity of 38 Vitis vinifera L. cultivars and hybrids originating in North America and Europe, including cultivars selected in Poland, which have not been characterized with the use of DNA markers yet. The agrobiological features of the genotypes selected for testing indicate that they may be useful for the breeding of new cultivars and grape production. The use of 12 ISSR primers allowed to obtain 94.4% of polymorphism. The polymorphic information content (PIC) value was high and varied between 0.829 and 0.953 with an average of 0.897. The resolving power (Rp) ranged between 3.678 and 8.892 with an average of 6.347. Primers UBC 809, UBC 810, UBC 812, UBC 855, UBC 891 and UBC 810 were found to be highly effective (informative). Similarity coefficient ranged between 0.167 and 1.0, which indicates high degree of diversity of tested grape cultivars. Tested cultivars were grouped in 3 main clusters; one of them was further divided into 6 subclusters. ‘Pannonia Kincse’ and ‘Danmarpa Polonia’ were not differentiated. Phenotypic differences among those two cultivars suggest that ‘Danmarpa Polonia’ might be a clone of ‘Pannonia Kincse’ and other molecular techniques must be used to differentiate them. Morphological and agrobiological characters of cultivars support the results obtained by ISSR markers.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 5; 141-152
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genetic diversity and relationship of Hunan province of China local tree peonies based on SSR markers
    Autorzy:
    Zhang, M.-H.
    Jin, X.-L.
    Wen, Y.-F.
    Shen, S.
    Wen-Xing
    Wu, S.
    Lu, J.-H.
    Ye-Ye
    Powiązania:
    https://bibliotekanauki.pl/articles/12665369.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    China
    Hunan Province
    plant cultivation
    tree peony
    Paeonia suffruticosa
    ornamental plant
    genetic diversity
    genetic relationship
    SSR marker
    Opis:
    Paeonia sect. Moutan is a wide known ornamental plant in the world. The objective of this study was to provide the theoretical basis for scientific preservation and utilization of tree peony resources of Hunan province of China. Simple sequence repeat (SSR) markers were applied to reveal the genetic diversity and relationship of 21 tree peony resources and 45 domestic and foreign tree peony cultivars. Clear bands, the size of which ranged from 115 bp to 379 bp, were detected with 14 primers. In total, 90 alleles were detected and the number of alleles detected with one primer varied between 5 and 13; the number of effective alleles ranged from 1.183 to 2.070; the polymorphism ratio of each locus was 100%. The observed heterozygosity, which ranged from 0.120 to 0.851 with an average of 0.532, was larger than the expected one, which ranged from 0.090 to 0.470 with an average of 0.300. Shannon index ranged from 0.137 to 0.695 and fixation index ranged from −0.332 to −0.869. The results show abundant genetic diversity in tree peony of Hunan province and SSR markers distinguishing homonymous tree peony resources successfully.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 4; 213-223
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-13 z 13

      Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies