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    Wyświetlanie 1-6 z 6
    Tytuł:
    The effect of growth retardants applied in vitro on the acclimatization and growth of Tibouchina urvilleana Cogn. in vivo
    Wpływ retardantów wzrostu zastosowanych in vitro na aklimatyzację i wzrost Tibouchina urvilleana Cogn. w warunkach in vivo
    Autorzy:
    Kozak, D
    Powiązania:
    https://bibliotekanauki.pl/articles/11364548.pdf
    Data publikacji:
    2006
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    in vitro
    acclimatization
    growth retardant
    plant cultivation
    growth
    in vivo
    Tibouchina urvilleana
    Opis:
    Shoot tips of Tibouchina urvilleana Cogn. were cultured 4 weeks in vitro in modified Murashige and Skoog (MS) [1962] medium supplemented with growth retardants: paclobutrazol – 0.1, 0.5, 1.0, 5.0 mg·dm⁻³, flurprimidol – 0.1, 1.0, 5.0 mg·dm⁻³, chlorocholine chloride (CCC) – 5.0, 50.0, 250.0 mg·dm⁻³. Explants cultured on the medium without growth substances were used as a control. Rooted microcuttings were transferred to the greenhouse and transplanted into a mixture of 1 peat : 1 perlite, where they were grown for 5 weeks. Plants were then cultivated in a peat substrate during another 5 weeks. Acclimatization of rooted shoots in the greenhouse was affective in 92.5–100%. The survival of plants was lowest when microcuttings were previously cultured on medium with flurprimidol at 5.0 mg·dm⁻³. Cultivation of Tibouchina urvilleana shoots in vitro on media with various growth retardants had a significant effect on the further growth of plants ex vitro. Paclobutrazol and flurprimidol at 5.0 mg·dm⁻³ inhibited very strong growth of plants after 10 weeks of growth ex vitro.
    Wierzchołki pędów Tibouchina urvilleana Cogn. prowadzono 4 tygodnie in vitro na zmodyfikowanej pożywce Murashige i Skooga (MS) [1962] z dodatkiem retardantów wzrostu: paklobutrazol – 0,1; 0,5; 1,0; 5,0 mg·dm⁻³, fluropirimidol – 0,1; 1,0; 5,0 mg·dm⁻³, chlorek chlorocholiny (CCC) – 5,0; 50,0; 250,0 mg·dm⁻³. Kontrolę stanowiła pożywka nie zawierająca retardantów wzrostu. Ukorzenione pędy przenoszono do szklarni i sadzono do mieszaniny torfu i perlitu (1:1), gdzie rosły przez 5 tygodni. Następnie rośliny przesadzono do substratu torfowego i uprawiano przez kolejne 5 tygodni. Rośliny przyjęły się w 92,5–100,0%. Najniższy procent przyjęć wykazywały rośliny pochodzące z pożywki zawierającej 5,0 mg·dm⁻³ fluropirimidolu. Wzrost in vitro pędów Tibouchina urvilleana na pożywce zawierającej retardanty wzrostu miał istotny wpływ na dalszy wzrost roślin w podłożu. Paklobutrazol i fluropirimidol w stężeniu 5,0 mg·dm⁻³ bardzo silnie hamowały wzrost roślin ex vitro po 10 tygodniach.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2006, 05, 1; 65-70
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Sustainable micropropagation of selected Stevia rebaudiana Bertoni genotypes
    Autorzy:
    Kaplan, B.
    Duraklioglu, S.
    Turgut, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/12691030.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    Turkey
    plant cultivation
    Compositae
    Stevia rebaudiana
    steviol glycoside
    sweetener
    micropropagation
    in vitro propagation
    growth regulator
    genotype
    Gelrite preparation
    Opis:
    Stevia rebaudiana Bertoni is a perennial plant belonging to Asteraceae family and its leaves contain steviol glycosides (SGs) that are 150 to 300 times sweeter than sucrose. The sweeteners obtained from S. rebaudiana can be safely used by diabetics as insulin secretion is not required during digestion of this sweetener. As it has zero calories, it is also used in diet products. Adaptation studies for Stevia conducted in Antalya, Turkey have shown that the stevia plant could easily be cultivated as a perennial. However, the lack of a sustainable vegetative propagation method creates a significant problem for stevia production. In the generatively populations, homogeneity and therefore quality are decreased because of cross-pollination. Stevia, as a self-incompatible and cross-pollinated species, has been shown to have very high genetic diversity. Therefore, development of a sustainable in vitro propagation method to prevent genetic heterogeneity of selected varieties is crucial for stevia cultivation. The aim of this study was to evaluate 2 different gelling agents (plant agar and Gelrite) and 20 different growth regulators combinations. The results demonstrated an approximately 200-fold multiplication rate obtained within 13 weeks using MS medium supplemented with 0.5 mg·dm–3 BAP and 0.25 mg·dm–3 kinetin and solidified with Gelrite. Average stevioside and rebaudioside A contents in in vitro propagated plant samples were found to be 8.1% and 8.6%, respectively.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 6; 47-56
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Fast direct regeneration of plants from nodal explants of Stevia rebaudiana Bert.
    Autorzy:
    Doliński, R.
    Kowalczyk, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/12611010.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    plant cultivation
    Stevia rebaudiana
    plant regeneration
    plant use
    medicinal plant
    nodal explant
    micropropagation
    in vitro culture
    auxin concentration
    Opis:
    In the preceding research, stevia has been typically cloned in vitro using two media, on which the shoots were formed (3–6 weeks), and on the other they were rooted (3–5 weeks). This study aimed at finding the possibility for rapid stevia propagation from large nodal explants using the MS basal medium [Murashige and Skoog 1962], with low auxin concentrations (0.5, 1 and 2 mg.dm–3). The plants were obtained as soon as after three weeks. The best results were obtained from media with various concentrations of the indole-3-acetic acid (IAA) and the highest concentration of phenylacetic acid (PAA). Plants were formed by 83.9–86.0% of explants, they had high weight (234–253 mg), two shoots measuring 2.07–2.37 cm and 5.8–8.3 roots measuring 1.00–1.24 cm. Mean plant weight was the lowest on media with indole-3-butyric acid (IBA) (185–192 mg). Both explant buds formed single shoots, but their development was typically uneven. The differences in the length and weight of shoots were the lowest on media with IAA and at lower PAA concentrations. Plants from the media with IAA and the control medium were distinguished by higher number of nodes. The percentage share of shoots in the total plant weight was the highest on media with PAA (62.1–62.7%), and the lowest at higher concentrations of α-naphthaleneacetic acid (NAA) (47.9 and 48.9%). Parts of explants immersed in media developed callus, and the highest amounts of this tissue were found in the media with NAA. 92.3% of plants survived the acclimatization. The applied procedure may be used for rapid in vitro cloning of selected stevia genotypes. The use of one medium enables reduction of seedling production costs. Moreover, cyclical cloning and extending the production scale is possible.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 5; 95-103
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro proliferation and ex vitro rooting of microshoots of lisianthus using auxin and cytokinin on solid, liquid and double-phase culture systems
    Autorzy:
    Kaviani, B.
    Bahari, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/12678778.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    plant cultivation
    Gentianaceae
    Eustoma
    Lisianthus
    Eustoma grandiflorum
    ornamental plant
    flowering plant
    rooting
    microshoot
    micropropagation
    in vitro proliferation
    ex vitro method
    soild phase
    liquid phase
    double-phase culture
    plant growth regulator
    auxin
    cytokinin
    Opis:
    A protocol was developed for high frequency and low cost of in vitro shoot proliferation and ex vitro rooting of Eustoma grandiflorum (Gentianaceae) on solid medium. Shoot tips as explants were cultured on Murashige and Skoog (MS) medium enriched with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) (0.00, 0.01, 0.10 and 1.00 mg l–1) and 6-benzylaminopurine (BAP) (0.00, 0.50, 2.00 and 5.00 mg l–1). Three culture media systems (solid, liquid and double-phase) were applied. None of the explants cultured on liquid and double-phase media resulted in live plant production. Maximum axillary shoot number (54.45) was recorded in the plantlets treated with 0.10 mg l–1 2,4-D in combination with 5.00 mg l–1 BAP. Treatment of 0.01 mg l–1 2,4-D along with 0.50 mg l–1 BAP produced maximum node number and internode length. Some shoots produced on medium containing plant growth regulators (PGRs) were rooted in soil. The largest number (5.50/plantlet) and longest length of root (7.75 cm/plantlet) were obtained in ex vitro condition on the base of shoots produced in culture medium enriched with 0.10 mg l–1 2,4-D along with 0.50 mg l–1 BAP. The combination of 1.00 mg l–1 2,4-D and 0.50 mg l–1 BAP was found to be the most suitable PGRs for obtaining the highest callus weight. The most fresh weight was calculated from plantlets grown on the medium containing 0.10 mg l–1 2,4-D along with 5.00 mg l–1 BAP. Maximum dry weight was obtained in free-PGRs medium. About 90% of the rooted plantlets were established successfully in cultivation beds. Acclimatized plants were morphologically similar to the mother plants.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 4; 47-56
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The influence of explants type and orientation on growth and development of Mandevilla sanderi (Hemsl.) Woodson in vitro
    Autorzy:
    Kozak, D.
    Parzymies, M.
    Świstowska, A.
    Marcinek, B.
    Ismael, B.S.
    Powiązania:
    https://bibliotekanauki.pl/articles/12663898.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    plant cultivation
    Brazilian jasmine
    Mandevilla sanderi
    ornamental plant
    flowering plant
    pot plant
    commercial plant
    plant growth
    plant development
    plant regeneration
    explant type
    multiplication
    decapitation
    defoliation
    in vitro culture
    tissue culture
    cultivation experiment
    Opis:
    Mandevilla sanderi is an important commercial ornamental pot plant. Traditional vegetative propagation is limited due to the low rate, therefore there is a need to develop an alternative, more efficient method. There is an interest in development of micropropagation technology for the species, as it allows to obtain a lot of offsprings in a relatively short time. The aim of the present work was to estimate an influence of explants type and position on regeneration of Mandevilla sanderi in tissue culture. Four different types of explants (leafy shoot tips, decapitated leafy shoot tips, defoliated shoot tips, decapitated and defoliated shoot tips) were used in the experiment, which were placed on the media vertically, while defoliated shoot tips were placed horizontally or vertically upside down. The explants were cultivated on a Murashige and Skoog (MS) medium supplemented with 1 mg·dm–3 benzyladenine (BA) and 0.5 mg·dm–3 indole-3-butyric acid (IBA). It was noted that both explants orientation and positioning, influenced the multiplication rate. Defoliated shoot tips placed horizontally were characterized by higher multiplication rate (6.8) in comparison to upside down vertical positioning (3.2). It was also observed that removal of shoot apex improved axillary branching, while defoliation of shoots placed in a normal position reduced multiplication rate.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 4; 111-119
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Evaluation of antioxidant activity of in vitro propagated medicinal Ceratophyllum demersum L. extracts
    Autorzy:
    Emsen, B.
    Dogan, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/11887732.pdf
    Data publikacji:
    2018
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    plant cultivation
    Ceratophyllaceae
    hornwort
    Ceratophyllum demersum
    plant propagation
    in vitro propagation
    plant regeneration
    plant extract
    antioxidant activity
    methanol extract
    metal chelating
    medicinal use
    reducing power
    Opis:
    Ceratophyllum demersum L. is a medical plant of the family Ceratophyllaceae that has been traditionally used for the treatment of diseases such as ulcer, diarrhoea, wounds and fever since ancient times. This study presents antioxidant activities of water and methanol extracts of in vitro propagated C. demersum. Shoot tip explants of C. demersum for in vitro plant regeneration were cultured on liquid Murashige and Skoog (MS) medium containing 0.25–1.25 mg/L 6-Benzylaminopurine (BAP) and 0.10 mg/L Thidiazuron (TDZ) combinations for eight weeks. Maximum number of plantlets per explant (110.67) was obtained on MS medium supplemented with 0.75 mg/L BAP + 0.10 mg/L TDZ. In order to determine antioxidant activities of C. demersum, antioxidant contents (β-carotene, flavonoid, lycopene and total phenols), metal chelating and reducing power capacities of methanol and water extracts obtained from C. demersum were investigated. It was detected that water extract was more effective in all activities. Total phenols (76.55 μg/mg) in water extract were measured as the highest antioxidant component. Based on the water extracts data, as IC50 value for chelating on ferrous ions was 9.24 mg/mL, EC50 value for reducing power activity was 8.23 mg/mL. It was also determined that the metal chelating and reducing power activities of the both extracts are increased depending on the concentration. Therefore, all these data suggest that both tested extracts of in vitro propagated C. demersum would exert beneficial effects by virtue of their antioxidant properties and may be utilized as a promising source of therapeutics.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2018, 17, 1; 23-33
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-6 z 6

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