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Wyświetlanie 1-4 z 4
Tytuł:
A fast and effective protocol for obtaining genetically diverse stevia (Stevia rebaudiana Bertoni) regenerants through indirect organogenesis
Szybki i efektywny protokół otrzymywania zróżnicowanych genetycznie regenerantów stewii (Stevia rebaudiana Bertoni) drogą pośredniej organogenezy
Autorzy:
Dyduch-Siemińska, M.
Powiązania:
https://bibliotekanauki.pl/articles/13925501.pdf
Data publikacji:
2021
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:
stevia
Stevia rebaudiana
micropropagation
molecular marker
random amplified polymorphic DNA
shoot regeneration
somaclonal variation
Źródło:
Agronomy Science; 2021, 76, 4; 47-62
2544-4476
2544-798X
Pojawia się w:
Agronomy Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Mikrorozmnażanie stewii (Stevia rebaudiana Bert.) z eksplantatów węzłowych izolowanych z roślin wytworzonych in vitro
Micropropagation of stevia (Stevia rebaudiana Bert.) through node explants isolated from in vitro produced plants
Autorzy:
Doliński, R.
Jabłońska, E.
Powiązania:
https://bibliotekanauki.pl/articles/11236698.pdf
Data publikacji:
2015
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:
mikrorozmnazanie
Stevia rebaudiana
odmiany roslin
aklimatyzacja roslin
rosliny mlode
in vitro
eksplantaty wezlowe
Źródło:
Annales Universitatis Mariae Curie-Skłodowska. Sectio E. Agricultura; 2015, 70, 4; 13-24
0365-1118
Pojawia się w:
Annales Universitatis Mariae Curie-Skłodowska. Sectio E. Agricultura
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Sustainable micropropagation of selected Stevia rebaudiana Bertoni genotypes
Autorzy:
Kaplan, B.
Duraklioglu, S.
Turgut, K.
Powiązania:
https://bibliotekanauki.pl/articles/12691030.pdf
Data publikacji:
2019
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:
Turkey
plant cultivation
Compositae
Stevia rebaudiana
steviol glycoside
sweetener
micropropagation
in vitro propagation
growth regulator
genotype
Gelrite preparation
Opis:
Stevia rebaudiana Bertoni is a perennial plant belonging to Asteraceae family and its leaves contain steviol glycosides (SGs) that are 150 to 300 times sweeter than sucrose. The sweeteners obtained from S. rebaudiana can be safely used by diabetics as insulin secretion is not required during digestion of this sweetener. As it has zero calories, it is also used in diet products. Adaptation studies for Stevia conducted in Antalya, Turkey have shown that the stevia plant could easily be cultivated as a perennial. However, the lack of a sustainable vegetative propagation method creates a significant problem for stevia production. In the generatively populations, homogeneity and therefore quality are decreased because of cross-pollination. Stevia, as a self-incompatible and cross-pollinated species, has been shown to have very high genetic diversity. Therefore, development of a sustainable in vitro propagation method to prevent genetic heterogeneity of selected varieties is crucial for stevia cultivation. The aim of this study was to evaluate 2 different gelling agents (plant agar and Gelrite) and 20 different growth regulators combinations. The results demonstrated an approximately 200-fold multiplication rate obtained within 13 weeks using MS medium supplemented with 0.5 mg·dm–3 BAP and 0.25 mg·dm–3 kinetin and solidified with Gelrite. Average stevioside and rebaudioside A contents in in vitro propagated plant samples were found to be 8.1% and 8.6%, respectively.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 6; 47-56
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Fast direct regeneration of plants from nodal explants of Stevia rebaudiana Bert.
Autorzy:
Doliński, R.
Kowalczyk, K.
Powiązania:
https://bibliotekanauki.pl/articles/12611010.pdf
Data publikacji:
2019
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:
plant cultivation
Stevia rebaudiana
plant regeneration
plant use
medicinal plant
nodal explant
micropropagation
in vitro culture
auxin concentration
Opis:
In the preceding research, stevia has been typically cloned in vitro using two media, on which the shoots were formed (3–6 weeks), and on the other they were rooted (3–5 weeks). This study aimed at finding the possibility for rapid stevia propagation from large nodal explants using the MS basal medium [Murashige and Skoog 1962], with low auxin concentrations (0.5, 1 and 2 mg.dm–3). The plants were obtained as soon as after three weeks. The best results were obtained from media with various concentrations of the indole-3-acetic acid (IAA) and the highest concentration of phenylacetic acid (PAA). Plants were formed by 83.9–86.0% of explants, they had high weight (234–253 mg), two shoots measuring 2.07–2.37 cm and 5.8–8.3 roots measuring 1.00–1.24 cm. Mean plant weight was the lowest on media with indole-3-butyric acid (IBA) (185–192 mg). Both explant buds formed single shoots, but their development was typically uneven. The differences in the length and weight of shoots were the lowest on media with IAA and at lower PAA concentrations. Plants from the media with IAA and the control medium were distinguished by higher number of nodes. The percentage share of shoots in the total plant weight was the highest on media with PAA (62.1–62.7%), and the lowest at higher concentrations of α-naphthaleneacetic acid (NAA) (47.9 and 48.9%). Parts of explants immersed in media developed callus, and the highest amounts of this tissue were found in the media with NAA. 92.3% of plants survived the acclimatization. The applied procedure may be used for rapid in vitro cloning of selected stevia genotypes. The use of one medium enables reduction of seedling production costs. Moreover, cyclical cloning and extending the production scale is possible.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 5; 95-103
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-4 z 4

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