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    Wyświetlanie 1-5 z 5
    Tytuł:
    Myxosporean infection of Grey Mullet in the Ebro Delta: Identification and Ultrastructure of Myxobolus ichkeulensis Bahri & Marques, 1996 Infecting the Gills of Mugil cephalus L.
    Autorzy:
    Maillo-Bellón, Pedro-Andrés
    Powiązania:
    https://bibliotekanauki.pl/articles/763664.pdf
    Data publikacji:
    2011
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    Fish parasite, aquaculture, Myxozoa, ultrastructure, Myxobolus ichkeulensis, Mugil cephalus
    Opis:
    The ultrastructural characteristics of the sporogenesis of Myxobolus, which infect the brachial arches of Mugil cephalus, is presented. The prevalence of infection was 52.7%. The ultrastructural features of the Myxobolus species studied in general comply with known features of this genus. Based on the ultrastructural morphology and specificity to the host organ, we conclude that this species is Myxobolus ichkeulensis (Bahri and Marques 1996).
    Źródło:
    Acta Protozoologica; 2011, 50, 1
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    High-Density Cultivation of the Marine Ciliate Uronema marinum (Ciliophora, Oligohymenophorea) in Axenic Medium
    Autorzy:
    ZHENG, Weibo
    Gao, Feng
    Warren, Alan
    Powiązania:
    https://bibliotekanauki.pl/articles/763770.pdf
    Data publikacji:
    2015
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    Axenic cultivation, ciliates, fish parasite, scuticociliatosis, Uronema marinum
    Opis:
    Uronema marinum is a cosmopolitan marine ciliate. It is a facultative parasite and the main causative agent of outbreaks of scuticociliatosis in aquaculture fish. This study reports a method for the axenic cultivation of U. marinum in high densities in an artificial medium comprising proteose peptone, glucose and yeast extract powder as its basic components. The absence of bacteria in the cultures was confirmed by fluorescence microscopy of DAPI-stained samples and the failure to recover bacterial SSU-rDNA using standard PCR methods. Using this axenic medium, a maximum cell density of 420,000 ciliate cells/ml was achieved, which is significantly higher than in cultures using living bacteria as food or in other axenic media reported previously. This method for high-density axenic cultivation of U. marinum should facilitate future research on this economically important facultative fish parasite.
    Źródło:
    Acta Protozoologica; 2015, 54, 4
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Ultrastructural Re-description of Henneguya piaractus (Myxozoa), a Parasite of the Freshwater Fish Piaractus mesopotamicus (Teleostei, Characidae) from the Paraguai River, Brazil
    Autorzy:
    AZEVEDO, Carlos
    Marques, Débora K. S.
    Casal, Graça
    Amaral, Cristiane M. C.
    Silva, Edinael V.
    Matos, Patrícia
    Matos, Edilson
    Powiązania:
    https://bibliotekanauki.pl/articles/763491.pdf
    Data publikacji:
    2010
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    Brazilian fish, Henneguya piaractus, Myxozoa, parasite, ultrastructure
    Opis:
    Ultrastructural analyses of fish-infecting myxosporean Henneguya piaractus that is found in the gill lamellae of the freshwater teleost Piaractus mesopotamicus (Characidae) and collected from the Paraguai River, Brazil were described. The parasite occurs within large whitish spherical to ellipsoidal polysporic cysts (up to 2.5 mm long) delimited by a layer of fibroblasts generally connected with some capillaries on the gill epithelium. No external morphological signs of disease were visible in the infected fishes. The tailed spores measured 61.5 ± 0.91 (60.2–62.6) μm in total length and ellipsoidal spore body 21.1 ± 0.62 (20.6–21.9) μm long, 6.7 ± 0.40 (6.2–7.3) μm wide and 2.5 ± 0.54 (2.0–3.1) μm thick. The spore wall was about 97 nm of thickness and consisted of a thin electron-dense exospore and a thick electron-lucent endospore with about 85 nm of thickness. The tailed spores were composed of two equal–sized shell valves adhering together along the straight suture line each having in continuity a equal caudal tapering tail measuring 40.5 ± 1.02 (38.7–43.1) μm in length. Two symmetric polar capsules measured 9.8 ± 0.28 (9.3–10.1) μm long and 1.9 ± 0.37 (1.4–2.4) μm wide, each having a polar filament with 10–11 (rarely 12) coils
    Źródło:
    Acta Protozoologica; 2010, 49, 2
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Ultrastructure of the Plasmodial Development of Myxobolus insignis (Myxozoa), Infecting the Amazonian Fish Semaprochilodus insignis (Prochilodontidae)
    Autorzy:
    AZEVEDO, Carlos
    De São Clemente, Sérgio Carmona
    Casal, Graça
    Matos, Patrícia
    OLIVEIRA, Elsa
    AL-QURAISHY, Saleh
    Matos, Edilson
    Powiązania:
    https://bibliotekanauki.pl/articles/763479.pdf
    Data publikacji:
    2013
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    Amazonian Region, fish, gill, plasmodium, myxosporean, parasite, ultrastructure
    Opis:
    This study used light and electron microscopy to describe a myxosporean, polysporic, histozoic plasmodium infecting the gill filaments of the freshwater teleost, Semaprochilodus insignis, specimens of which were collected from the Trombetas River (Central Amazonian Region, Brazil). Ultrastructural analyses of the fish-infecting spores identified the parasite as Myxobolus insignis, an organism that occurs within whitish unequal-sized plasmodia located in the intralamellar epithelium of the gill. Based on the observed morphological and ultrastructural features of the plasmodia in this study three stages in the plasmodial evolution were distinguished, related to the sporogonic stages of Myxobolus insignis. The plasmodium walls were also found to constitute a number of layers of fibroblasts, surrounded by collagen fibres, which displayed different morphological arrangements according to the different phases of evolution. This represents the first time such ultrastructural features have been described in detail for Myxobolus insignis plasmodia and offers potentially significant points of comparison with plasmodia from other species of myxosporea.
    Źródło:
    Acta Protozoologica; 2013, 52, 2
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Description of Triangulamyxa psittaca sp. n. (Myxozoa: Myxosporea), a new parasite in the urinary bladder of Colomesus psittacus (Teleostei) from the Amazon River, with emphasis on the ultrastructure of plasmodial stages
    Autorzy:
    Rocha, Sónia
    Casal, Graça
    Matos, Patrícia
    Matos, Edilson
    Dkhil, Mohamed A.
    AZEVEDO, Carlos
    Powiązania:
    https://bibliotekanauki.pl/articles/763644.pdf
    Data publikacji:
    2011
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    Ultrastructure, plasmodia, myxosporean, Triangulamyxa psittaca sp. nov., parasite, urinary bladder, freshwater fish, Colomesus psittacus
    Opis:
    A fish-infecting myxosporean was found in the urinary bladder of the teleostean Colomesus psittacus, collected from the Amazon River, Brazil. Specimens were sampled in three different periods: May and June, with water temperature ranging from 18–23ºC; August, with water temperature ranging from 24–28ºC; and November and December, with water temperature ranging from 29–32ºC. Upon observation, several fish displayed abnormal behaviour, consisting of erratic movements, and mortality was recorded among them. Necropsy of all sampled fishes revealed hypertrophy of the urinary bladder only among specimens previously displaying the irregular behaviour. Microscopic analysis of this organ confirmed the parasitic infection, resulting in the observation of spores floating free in the urine, and numerous plasmodia attached to the epithelium of the urinary bladder. Light and ultrastructural studies allowed recognition of the spores and plasmodia morphological characteristics. Coelozoic plasmodia were polysporic with varying organizational structure, according to the sampling period. Spores were equilaterally triangular with rounded ends in valvar view, measuring 8.8 ± 0.4 μm (n = 30) in length and 8.4 ± 0.5 μm (n = 30) in width, and displaying a ridge surface pattern. Two polar capsules were observed in the anterior end of the spores, measuring 3.1–3.2 μm in diameter. The spores were morphologically identified as belonging to the recently described genus Triangulamyxa. Further observation and comparison to the morphological features described for Triangulamyxa amazonica, the only other species within this genus, allowed us to conclude our parasite as a new species, herein named Triangulamyxa psittaca sp. nov. from the Amazon River, Brazil. Also, three different stages were distinguished in the plasmodium evolution, based on the observed morphological features at the three sampling periods. Fish sampled during May and June displayed small plasmodia (up to ~ 15–20 μm long), containing early stages of sporogenic development. Fish sampled during November and December presented larger plasmodia (up to ~ 850 μm long), which appeared flattened against and lining the urinary bladder epithelial cells and contained the later stages of sporogenic development, including some mature spores. Fish sampled during August presented plasmodia displaying intermediate morphological features between those observed in infected fish from the other sampling periods. Several immature and mature spores were among the different developmental stages. The parasite-host interface evolution is described throughout the different observed stages, with emphasis on the formation of septate junctions. Considering several previous reports, as well as the different environmental conditions during the sampling periods, the plasmodium development here described appears to be influenced by environmental factors, namely water temperature.
    Źródło:
    Acta Protozoologica; 2011, 50, 4
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-5 z 5

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