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    Tytuł:
    Molecular Phylogenetics Evidence for a Novel Lineage of Amoebae Within Discosea (Amoebozoa: Lobosa)
    Autorzy:
    Corsaro, Daniele
    Venditti, Danielle
    Powiązania:
    https://bibliotekanauki.pl/articles/763405.pdf
    Data publikacji:
    2013
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    Amoebozoa, environmental 18S rDNA, small amoebae, Dermamoebida
    Opis:
    Some amoebae were recovered from freshwater samples on agar plates. Due to a fungal contamination tightly associated with these amoebae, it was impossible to correctly characterize them on a morphological base, but sequences of the small subunit ribosomal RNA gene (SSU rDNA) were successfully obtained from three strains. Phylogenetic analysis performed on these SSU rDNA allowed to identify these amoebae as members of a new lineage, related to the Dermamoebida, which includes also several other environmental SSU sequences.
    Źródło:
    Acta Protozoologica; 2013, 52, 4
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Molecular Characterization of Two Myxosporean Species, Henneguya namae Haldar et al. 1983 and Myxobolus sophorae Jayasri, 1982 (Myxosporea: Myxobolidae)
    Autorzy:
    Garg, Anupma
    Chaudhary, Anshu
    Gupta, Abhishek
    Kumar, Abhinav
    Sharma, Bindu
    Singh, Hridaya Shanker
    Powiązania:
    https://bibliotekanauki.pl/articles/52057885.pdf
    Data publikacji:
    2020
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    fish
    Henneguya
    India
    Myxobolus
    phylogeny
    18S rDNA
    Opis:
    In Indian freshwater fish myxosporean infections are among the most cosmopolitan parasites, they are relatively well studied morphologically but their phylogenetic relationships were unclear and the genetic data is limited only to a few species. The study aims to present molecular data for two myxosporean species, Henneguya namae Haldar et al. 1983 and Myxobolus sophorae Jayasri, 1982 collected from Indian freshwater fish, the elongate glass-perchlet Chanda nama (=Ambassis nama) and pool barb Puntius sophore, respectively. In the present study molecular data are provided for H. namae and M. sophorae using nested PCR. The obtained partial 18S rDNA gene sequences were analyzed using maximum likelihood (ML) and Bayesian inference (BI) methods. The 18S rDNA gene sequences of H. namae showed similarity with the sequences of H. chaudhuryi, Henneguya sp. RA-2015, H. voronini and H. setiuensis about 72.1 to 78% and M. sophorae with Myxobolus ticto was about 90% respectively. The aim of this paper was to identify H. namae and M. sophorae morphologically and using molecular methods.
    Źródło:
    Acta Protozoologica; 2020, 59, 1; 39-53
    0065-1583
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Morphology, Ciliary Pattern and Molecular Phylogeny of Trachelophyllum brachypharynx Levander, 1894 (Litostomatea, Haptoria, Spathidiida)
    Autorzy:
    JANG, Seok Won
    VĎAČNÝ, Peter
    SHAZIB, Shahed Uddin Ahmed
    SHIN, Mann Kyoon
    Powiązania:
    https://bibliotekanauki.pl/articles/763656.pdf
    Data publikacji:
    2015
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    18S rRNA gene, dorsal brush, Korea, lepidosomes, Trachelophyllidae
    Opis:
    We isolated a relatively unknown haptorian ciliate, Trachelophyllum brachypharynx, in brackish water from the mouth of the Taehwa River, South Korea. The morphology of this isolate was studied using in vivo observation and protargol impregnation, and its evolutionary history was revealed by phylogenetic analysis of the 18S rRNA gene. The main features of T. brachypharynx include (i) a very narrowly fusiform and slightly contractile body about 380 × 40 μm in size; (ii) two ellipsoidal macronuclear nodules typically connected by a fine strand; (iii) a single terminal contractile vacuole; (iv) filiform extrusomes that are typically 30 µm long; (v) an average of 24 ciliary rows, with two of them anteriorly differentiated into an isostichad dikinetidal dorsal brush; and (vi) hat-shaped lepidosomes. Based on the 18S rRNA gene phylogeny, T. brachypharynx clustered together with Trachelophyllum sp. within the order Spathidiida. Furthermore, phylogenetic trees and networks indicate some members from the genera Enchelyodon and Spathidium as the nearest relatives of trachelophyllids. Therefore, based on the present molecular and comparative-morphological analyses, we suggested a hypothesis explaining how trachelophyllids may have evolved from a spathidiid-like ancestor via an enchelyodonid-like stage.
    Źródło:
    Acta Protozoologica; 2015, 54, 2
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Description of Epistylis camprubii n. sp., a Species Highly Tolerant to Ammonium and Nitrite
    Autorzy:
    Canals, Oriol
    Salvadó, Humbert
    Powiązania:
    https://bibliotekanauki.pl/articles/763511.pdf
    Data publikacji:
    2016
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    Peritrichia, wastewater treatment, ciliates, 18s rRNA, ammonium, nitrite
    Opis:
    A new peritrich species highly tolerant to ammonium and nitrite, Epistylis camprubii n. sp., was found adhered to the biofilm of two advanced wastewater treatment plants treating high ammonium-loaded wastewater in Rubí, Spain. Its morphology, oral infraciliature and phylogenetic position in the peritrich clade were studied. The new species is a vase-shaped peritrich, constricted below the peristomial lip, with an in vivo average length of 58.7 ± 10.1 µm, average width of 32.0 ± 5.4 µm, and a longitudinally striated, compact stalk that occasionally exhibits uneven thickness and rarely shows transverse segments. The peristomial disc is commonly rounded or pointed, and rarely umbilicated. The C-shaped macronucleus is located in the adoral half of the body, and the only contractile vacuole lies in the adoral third of the zooid. The molecular analysis of the 18s gene sequence clustered E. camprubii n. sp. together with the other Epistylis, with the exception of Epistylis galea.
    Źródło:
    Acta Protozoologica; 2016, 55, 1
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Morphology and Molecular Analyses of a New Marine Ciliate, Arcuseries minima sp. nov. (Ciliophora: Urostylidae)
    Autorzy:
    Kim, Kang-San
    Chae, Kyu-Seok
    Min, Gi-Sik
    Powiązania:
    https://bibliotekanauki.pl/articles/52090014.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    Marine ciliate
    Arcuseries
    phylogenetic analysis
    urostylid
    18S rDNA sequence
    Opis:
    A new marine urostylid ciliate, Arcuseries minima sp. nov., was discovered in South Korea. Morphological observations and molecular phylogenetic analyses based on small subunit ribosomal DNA (18S rDNA) sequences were used to describe the new species. Arcuseries minima is most similar to A. scutellum among all   Arcuseries species, but differs in the following main characters: number of adoral membranelles (13–16 vs. 17 or 18), cortical granules (yellowish, clustered around cirri and dorsal bristles vs. colorless, irregularly scattered), number of macronuclear nodules (20–27 vs. 42–90), number of midventral cirri (5–10 vs. 12–14), and number  f transverse cirri (5 or 6 vs. 8). The new species and A. scutellum differ from A. petzi and A. warreni in having smaller body size (≤80 μm vs. ≥80 μm) and fewer cirri: left marginal (≤ 17 vs. ≥ 18) and transverse (≤ 8 vs. ≥8) cirri. This relationship was supported by the phylogenetic tree, where these two groups were separated into two branches.
    Źródło:
    Acta Protozoologica; 2019, 58, 2; 43-51
    0065-1583
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Reconstruction of Evolutionary History of Pleurostomatid Ciliates (Ciliophora, Litostomatea, Haptoria): Interplay of Morphology and Molecules
    Autorzy:
    VĎAČNÝ, Peter
    SHIN, Mann Kyoon
    KIM, Ji Hye
    JANG, Seok Won
    SHAZIB, Shahed Uddin Ahmed
    RAJTER, Ľubomír
    Powiązania:
    https://bibliotekanauki.pl/articles/763636.pdf
    Data publikacji:
    2015
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    18S rRNA gene, Acineria, Epiphyllidae fam. nov., Kentrophyllum, Korea
    Opis:
    Pleurostomatids are raptorial ciliates that form a very distinct group within the Haptoria. Traditionally, the order Pleurostomatida was divided into two families: the Amphileptidae with two perioral kineties and a suture formed by the right side ciliary rows, and the Litonotidae with three perioral kineties and without suture. However, molecular phylogenies depicted the “traditional” Amphileptidae as a paraphyletic assemblage nesting also the Litonotidae. To overcome this problem we have analyzed genealogy of pleurostomatids using morphological data and 18S rRNA gene sequences, including newly sequenced genera Acineria and Kentrophyllum. Specifically, we have combined a morphological and molecular approach and have used also some other phylogenetic tools such as phylogenetic networks, split spectrum analysis, quartet mapping as well as the likelihood method of tracing history of morphological characters. These analyses show that: (1) there are not two but three distinct pleurostomatid lineages – Epiphyllidae fam. nov., Amphileptidae and Litonotidae; (2) epiphyllids (Epiphyllum + Kentrophyllum) represent a basal pleurostomatid group which is defined by two perioral kineties, by the presence of a suture on both the right and the left side of the body, by the loss of the oral bulge extrusomes, and by the extrusome fringe extending all around the body except for the oral region; (3) the families Amphileptidae and Litonotidae are monophyletic each, and represent sister groups; (4) Acineria belongs to the Litonotidae, as already indicated by morphological data; (5) Loxophyllum is a monophyletic and crown genus of the Litonotidae; and (6) Litonotus is paraphyletic, which could be very likely caused by a rapid radiation event that did not allow primary nucleotide homologies to be fixed.
    Źródło:
    Acta Protozoologica; 2015, 54, 1
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Two new Myxobolus spp. (Myxozoa: Myxobolidae) from white bream, Blicca bjoerkna (Linnaeus, 1758) developing in basifilamental location of gills
    Autorzy:
    Molnár, Kálmán
    Eszterbauer, Edit
    Guti, Csaba F.
    Székely, Csaba
    Powiązania:
    https://bibliotekanauki.pl/articles/763533.pdf
    Data publikacji:
    2014
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    Myxozoa, site selection, tissue tropism, occurrence, histology, 18S rDNA, phylogeny
    Opis:
    Two new Myxobolus species, M. bjoerknae sp. n. and M. lamellobasis sp. n. have been described from the gills of white bream, Blicca bjoerkna. Plasmodia of M. bjoerknae sp. n. developed in the connective tissue inside the cartilaginous gill arch, while plasmodia of M. lamellobasis sp. n. seem to start their development in the multilayered epithelium between two lamellae close to the base of gill filaments. Then they may bulge out of the interlamellar space fused to a large bulk locating at the base of filaments. The large, ellipsoidal spores of M. bjoerknae sp. n. 17.4 × 13.1 µm in size, resembled the spores of other species developing in the gill arch (e.g. M. fundamentalis, M. gayerae, and M. pfeifferi), but differed from them in its 18S rDNA sequence. Roundish spores of M. lamellobasis sp. n. with a size of 11.1 × 8.6 µm resembled the spores of M. impressus developing interlamellarly and the spores of M. rotundus, M. parviformis, and M. muellericus having intralamellar localization. However, the detected genetic difference clearly distinguished it from the other species developing in similar tissue location. The phylogenetic location of the two newly described species seems to correlate both with spore shape and fish host species.
    Źródło:
    Acta Protozoologica; 2014, 53, 3
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Morpho-molecular Characterization of the Litostomatean Predatory Ciliate Phialina pupula (Müller, 1773) Foissner, 1983 (Haptoria, Lacrymariidae)
    Autorzy:
    Rajter, Ľubomír
    Bourland, William
    Vďačný, Peter
    Powiązania:
    https://bibliotekanauki.pl/articles/52076120.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    18S rRNA gene
    Boise
    floodplain sand
    ITS region
    Lacrymariidae
    phylogeny
    Opis:
    The morphology and phylogenetic position of a haptorian ciliate, Phialina pupula (Müller, 1773) Foissner, 1983, isolated from microaerobic sandy sediments of the floodplain area of the Boise River, Idaho, U.S.A., were studied using live observation, protargol impregnation, scanning electron microscopy, and the 18S rRNA gene as well as the ITS region. The Boise population of P. pupula is characterized by a size of about 60–130 × 20–50 μm, an elliptical macronucleus with a single micronucleus, highly refractive dumbbell-shaped inclusions scattered throughout the cytoplasm and concentrated in the anterior body half, a single subterminal/terminal contractile vacuole, about 10 μm long rod-shaped extrusomes, and an average of 15 ciliary rows. In phylogenetic analyses, the newly obtained sequences from P. pupula and Lacrymaria olor clustered within the family Lacrymariidae with full to moderate statistical support. Neither the genus Phialina nor the genus Lacrymaria was depicted monophyletic both in the single gene and multigene phylogenetic inferences. Specifically, the genus Phialina was shown as a paraphyletic assemblage containing members of the polyphyletic genus Lacrymaria. This indicates that the phialinid bauplan, i.e., an anterior body end differentiated into a head-like structure directly attached to the trunk, might represent the ground pattern in the family Lacrymariidae. On the other hand, the long highly contractile neck carrying the head-like structure probably evolved later and convergently in multiple Lacrymaria species from Phialina-like ancestors.
    Źródło:
    Acta Protozoologica; 2019, 58, 2; 53-68
    0065-1583
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A Hotspot of Amoebae Diversity: 8 New Naked Amoebae Associated with the Planktonic Bloom-forming Cyanobacterium Microcystis
    Autorzy:
    van Wichelen, Jeroen
    Bass, David
    Berney, Cédric
    Claeys, Myriam
    d’Hondt, Sofie
    Vanormelingen, Pieter
    Vyverman, Wim
    Powiązania:
    https://bibliotekanauki.pl/articles/763601.pdf
    Data publikacji:
    2016
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    Naked amoebae, Microcystis, morphology, 18S rDNA, harmful algal blooms, grazing, Europe
    Opis:
    The colonies of Microcystis, one of the most common bloom-forming cyanobacteria worldwide, harbor a diverse community of microorganisms. Among these, naked amoebae feeding on Microcystis cells can strongly influence natural Microcystis population dynamics. In this study, we investigated the species diversity of these amoebae based on 26 Microcystis-associated amoebae (MAA) strains from eutrophied water bodies in Belgium and elsewhere in western Europe. A detailed morphological characterization in combination with 18S rDNA (SSU) phylogenies revealed the presence of no less than 10 species. Nine of these belonged to the known genera Vannella (2 species), Korotnevella (2), Copromyxa (2), Vexillifera (1), Cochliopodium (1) and the recently described Angulamoeba (1). Only two were previously described, the others were new to science. One taxon could not be assigned to a known genus and is here described as Schoutedamoeba gen. n., representing a new variosean lineage. The discovery of so many new species from only one very specific habitat (Microcystis colonies) from a rather restricted geographical area indicates that the diversity of planktonic naked amoebae is much higher than previously appreciated and that only a tiny fraction of the total diversity of naked amoebae is currently known.
    Źródło:
    Acta Protozoologica; 2016, 55, 2
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A New Trichodina Species (Peritrichia: Mobilida) from Anuran Tadpole Hosts, Sclerophrys spp. in the Okavango Panhandle, Botswana, with Comments on this Taxon
    Autorzy:
    de Jager, Gerhard P.
    Basson, Linda
    van Marwijk, Jacqueline
    Powiązania:
    https://bibliotekanauki.pl/articles/52089989.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    Trichodina koloti sp. nov.
    Sclerophrys spp.
    morphology
    18S ribosomal DNA
    southern Africa
    Opis:
    Mobiline taxonomic data is mostly inferred from populations collected in and on hosts associated with aquaculture. Even though these conditions may be conducive for studies relating to the hosts, accurate taxonomic inferences of the symbionts will be problematic. The site for the present study was the Okavango Panhandle region in Northern Botswana, an isolated, natural area with minimal anthropogenic influences. Morphometric and molecular evidence revealed that anuran tadpole trichodinids, up to now reported as Trichodina heterodentata Duncan 1977 and T. hypsilepis Wellborn 1967 from multiple host types, are in fact a new, more host specific species. This study includes comprehensive denticle descriptions of both the anuran hosted trichodinid and the morphologically similar T. hypsilepis restricted to teleost hosts (previously T. heterodentata).
    Źródło:
    Acta Protozoologica; 2019, 58, 3; 141-153
    0065-1583
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-10 z 10

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