- Tytuł:
- Effects of arginine substitutions on the cardioinhibitory activity of the Led-NPF-I neuropeptide
- Autorzy:
-
Marciniak, P.
Slocinska, M.
Bednarz, P.
Grodecki, S.
Konopinska, D.
Rosinski, G. - Powiązania:
- https://bibliotekanauki.pl/articles/55015.pdf
- Data publikacji:
- 2009
- Wydawca:
- Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
- Tematy:
-
cardioinhibitory activity
arginine substitution
mealworm beetle
Zophobas atratus
giant mealworm beetle
Led-NPF-I-peptide
insect
beetle
neuropeptide - Opis:
- Effects of structural changes on the cardioinhibitory activity of the Led-NPF-I peptide (Ala-Arg-Gly-Pro-Gln-Leu-Arg-Leu-Arg-Phe-amide) were examined by replacing Arg residues in positions 2, 7 and 9. Replacement of L-Arg2 with another basic amino acid, such as Lys, His or D-Arg, did not abolish but rather promoted cardioinhibitory activity in giant mealworm beetle Zophobas atratus Fab. Agonistic peptides were also obtained by substitution of Arg residue in position 7 with Lys or D-Arg, and Arg in position 9 with His or D-Arg, respectively. All these analogues showed stronger cardioinhibitory effects than the native peptide at low concentration (10-9 M), and [Lys7]-, [D-Arg7]- and [D-Arg9]-Led-NPF-I also at the higher concentration (10-6 M). However, substitutions of the Arg residues in position 7 with His or in position 9 with Lys caused a loss of the cardioinhibitory activity. In addition, the replacement of Arg residues in all three positions with Lys or Orn caused a reduction of cardioinhibitory activity, although a single substitution of Arg in positions 2 or 7 with Lys yielded agonistic peptides. We conclude that the Arg2 position in the N-terminal region is more tolerant to structural modification than the other two Arg positions located in the C-terminal region.
- Źródło:
-
Pestycydy; 2009, 1-4; 71-78
0208-8703 - Pojawia się w:
- Pestycydy
- Dostawca treści:
- Biblioteka Nauki
Artykuł