- Tytuł:
- Phenobarbital analysis in biological matrix (blood) by high performance liquid chromatography (HPLC)
- Autorzy:
-
Hamza, O.
Mohamed Moktar, B.
Chohra Mostafa, B.
Zeghdaoui, A. - Powiązania:
- https://bibliotekanauki.pl/articles/412593.pdf
- Data publikacji:
- 2014
- Wydawca:
- Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
- Tematy:
-
Phenobarbital
HPLC
blood
extraction
biological matrix - Opis:
- In this work, we have tried to contribute to the analysis of phenobarbital in the blood. To do this, we used different analytical techniques such as liquid chromatography (HPLC). The results obtained in the course of our work, we can conclude that: The HPLC method was separate phenobarbital endogenous blood components, and optimizing the conditions of chromatographic separation as the composition of the mobile phase consisting of 20 % acetonitrile + 20 % methanol + 60 % ammonium acetate buffer. The extraction with diethyl ether to pH = 4; The chromatographic column, microbondapack ZORBAX C18. A system of diode-array UV detection at 254 nm.
- Źródło:
-
International Letters of Chemistry, Physics and Astronomy; 2014, 1; 31-40
2299-3843 - Pojawia się w:
- International Letters of Chemistry, Physics and Astronomy
- Dostawca treści:
- Biblioteka Nauki
Artykuł