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Wyszukujesz frazę "Bacillus" wg kryterium: Temat


Wyświetlanie 1-12 z 12
Tytuł:
Growth Characteristics and Tracing Antagonistic Properties of Bacillus flexus and Bacillus subtilis as Antibacterials to Overcome the Attack of Aeromonas hydrophila Bacteria on Fish
Autorzy:
Amatulloh, Zulfahana
Mulyani, Yuniar
Dewanti, Lantun Paradhita
Haetami, Kiki
Powiązania:
https://bibliotekanauki.pl/articles/1193472.pdf
Data publikacji:
2021
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Aeromonas hydrophila
Bacillus carboniphilus
Bacillus flexus
Bacillus haynesii
Bacillus subtilis
Bacillus tequilensis
Bacillus zhangzhouensis
Inhibition Zone Test
fish
Opis:
This research was conducted to assess the antibacterial potential of Bacillus flexus and Bacillus subtilis against Aeromonas hydrophila. Bacterial samples came from the stock of the FPIK Unpad Biotechnology Laboratory. This research was conducted in July 2020 - May 2021 in Laboratorium Biotechnology FPIK Padjadjaran University. This research was conducted using qualitative and quantitative descriptive analysis. Bacteria were collected using the purposive sampling method, Observation of the growth curve using the turbidimetric method, and the disc diffusion method for the inhibition zone test with bacterial supernatant treatment with a concentration of 50%, a concentration of 100%, bacterial culture, chloramphenicol antibiotics with a concentration of 4000 ppm as a positive control. The results showed that B. flexus bacteria experienced an exponential phase at 6 to 12 hours, after which it entered a stationary phase at 14 to 72 hours. B. subtilis bacteria experienced an exponential phase at 20 hours. Until 40 hours, then experiencing a stationary phase at 42 hours to 72 hours, and the growth of A. hydrophila bacteria experiences an exponential phase at 6 hours to 28 hours, the stationary phase at 30 to 28 hours 72. Based on the results of the inhibition zone test, it showed the formation of the inhibition zone only in the positive control treatment, while the supernatant and bacterial cultures of B. flexus and B. subtilis didn’t form an inhibition zone.
Źródło:
World Scientific News; 2021, 158; 91-104
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Growth characteristics and antagonistic potential of Bacillus cereus and Bacillus zhangzhouensis against pathogenic bacteria Aeromonas hydrophila in vitro
Autorzy:
Sofandi, Adrianti Nur Fitria
Mulyani, Yuniar
Rochima, Emma
Rosidah, Rosidah
Powiązania:
https://bibliotekanauki.pl/articles/1193481.pdf
Data publikacji:
2021
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Aeromonas hydrophila
Bacillus cereus
Bacillus zhangzhouensis
antimicrobial activity
pathogenic bacteria
Opis:
This study aims to examine the growth characteristics of B. cereus and B. zhangzhouensis and to see their potential in preventing the growth of Aeromonas hydrophila in vitro. This research was conducted in June 2020 - May 2021 at the Laboratory of Microbiology and Biotechnology, Faculty of Fisheries and Marine Sciences, Padjadjaran University. The method used is the exploratory method and the data obtained were analyzed descriptively. The growth curve was performed using the turbidimetric method. The inhibition test used the disc diffusion method with 5 treatments, chloramphenicol antibiotics (4000 ppm), distilled water, bacterial culture, 100% supernatant and 50% supernatant. The curve results showed that the growth of B. cereus and B. zhangzhouensis was slow. The stationary phase of B. cereus occurred at 40-72 hours, while in B. zhangzhouensis the stationary phase occurred at 48-72 hours. The results of the inhibition test showed that the antimicrobial activity of B. cereus and B. zhangzhouensis was weak with a value range of 0.71 - 1.6mm, besides that the results of the inhibition zone produced by these two bacteria have different inhibitory abilities in each treatment.
Źródło:
World Scientific News; 2021, 158; 187-200
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Screening of cellulase and pectinase by using Pseudomonas fluorescens and Bacillus subtilis
Autorzy:
Reetha, S.
Selvakumar, G.
Bhuvaneswari, G.
Thamizhiniyan, P.
Ravimycin, T.
Powiązania:
https://bibliotekanauki.pl/articles/11688.pdf
Data publikacji:
2014
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
screening
cellulase
pectinase
Pseudomonas fluorescens
Bacillus subtilis
Opis:
A study was conducted to determine the Production of cellulase and pectinase enzyme by using Plant growth promoting rhizobacteria like Pseudomonas fluorescence and Bacillus subtilis. These to micro organism are isolated by serial dilution method. One gram of soil sample was diluted in to 10 ml of sterile distilled water and 1 ml of sample solution was serially diluted in 9ml of sterile water up to 10 dilution. Each sample from dilution 10-5 and 10-6 were taken and streaked in to KB and NA medium and incubate at 24 hrs. After 24 hrs Pseudomonas fluorescence and Bacillus subtilis was observed in the medium of KB and NA medium. Both the culture was sub cultured and maintain in the same for the further work. CMCase medium was prepared and sterilized by autoclave for 121 ºC for 15 minutes after sterilization these medium contain petriplate was streaked by bacteria and incubates for 48h after incubation period a clear halo zone was produced by these bacteria among these bacteria Pseudomonas fluorescence are able to produce high amount of cellulose compare to Bacillus subtilis. Pectin agar medium was prepared and sterilized by autoclave for 121 ºC for 15 minutes after sterilization these medium contain petriplate was streaked by bacteria incubates for 48h after incubation period a clear halo zone was produced by these bacteria, among these bacteria Pseudomonas fluorescence are able to produce high amount of Pectinase compare to Bacillus subtilis. Plant growth promoting rhizobacteria (PGPR) are beneficial bacteria that colonize plant roots and enhance plant growth by a wide variety of mechanisms.
Źródło:
International Letters of Natural Sciences; 2014, 08, 2
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Bacilli bacterial cell image analysis using active contour segmentation with SVM classifier
Autorzy:
Bannigidad, Parashuram
Powiązania:
https://bibliotekanauki.pl/articles/1062913.pdf
Data publikacji:
2019
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Active contour method
Bacillus
Bacillus subtilis
Bacterial cell image analysis
Cell classification
Cocobacilli
Diplobacilli
Palisades
SVM
Segmentation
Streptobacilli
Opis:
The main aim of the present study is to develop an automatic method to identify and classify the different cell types of bacilli bacterial cells in digital microscopic cell images using active contour method. Snakes, or active contours, are used widely in computer vision and machine learning applications, particularly to locate object boundaries. GLCM, HOG and LBP features are used to identify the arrangement of bacilli bacterial cells, namely, bacillus, cocobacilli, diplobacilli, palisades and streptobacilli using SVM classifier. The current methods rely on the subjective reading of profiles by a human expert based on the various manual staining methods. In this paper, it is proposed a method for bacilli bacterial cell classification by segmenting digital bacterial cell images using active contour model and extracting GLCM, HOG and LBP features. The experimental results proves that, the SVM classifier has yielded an overall accuracy of 97.2% with GLCM features, HOG features has yielded an accuracy of 74.8% and LBP features yielded 91.2% accuracy. The GLCM features with SVM classifier has got good classification results compared to HOG and LBP feature sets for bacilli bacteria cell types.
Źródło:
World Scientific News; 2019, 127, 3; 369-376
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Virtual screening and docking studies of identified potential drug target: polysaccharide deacetylase in Bacillus anthracis
Autorzy:
Zaveri, K.
Chaitanya, A.K.
Reddy, I.B.
Powiązania:
https://bibliotekanauki.pl/articles/11463.pdf
Data publikacji:
2015
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
virtual screening
drug target
polysaccharide deacetylase
Bacillus anthracis
Opis:
In recent years, insilico approaches have been predicting novel drug targets. The present day development in pharmaceutics mainly ponders on target based drugs and this has been aided by structure based drug designing and subtractive genomics. In the present study, the computational genome subtraction methodology was applied for identification of novel, potential drug target against Bacillus anthracis, cause of deadly anthrax. The potential drug target identified through subtractive genomics approach was considered as polysaccharide deacetylase. By virtual screening against NCI database and Drugbank chemical libraries, two potential lead molecules were predicted. Further the potential lead molecules and target protein were subjected for docking studies using Autodock.
Źródło:
International Letters of Natural Sciences; 2015, 07
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Synthesis and antimicrobial activity of substituted (E)-1-(4-fluoro-3-methylphenyl)-3-phenylprop-2-en-1-one compounds
Autorzy:
Balaji, S.
Senbagam, R.
Rajarajan, M.
Vijayakumar, R.
Manikandan, V.
Vanangamud, G.
Thirunarayanan, G.
Powiązania:
https://bibliotekanauki.pl/articles/1189942.pdf
Data publikacji:
2016
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Antibacterial activity
Antifungal activity
Bacillus subtilis
vibrio cholera
Opis:
In an effort to develop antimicrobial agents, a series of substituted (E)-1-(4-fluoro-3-methylphenyl)-3-phenylprop-2-en-1-one compounds were synthesized by crossed-aldol condensation reaction of 4-fluoro-3-methyl acetophenone with various substituted benzaldehydes in the presence of sodium hydroxide. The synthesized compounds were characterized by means of their UV, IR and NMR spectral data. The synthesized substituted (E)-1-(4-fluoro-3-methylphenyl)-3-phenylprop-2-en-1-one compounds were tested for their antibacterial and antifungal activities by the Bauer-Kirby disc diffusion method.
Źródło:
World Scientific News; 2016, 52; 14-30
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effectiveness of Bacillus sp. to increase the body resistance of common carp (Cyprinus carpio Linnaeus, 1758) against the attack of Aeromonas hydrophila
Autorzy:
Insanie, Zahara Pujie
Mulyani, Yuniar
Handaka, Asep Agus
Rosidah, Rosidah
Powiązania:
https://bibliotekanauki.pl/articles/1062804.pdf
Data publikacji:
2019
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Aeromonas hydrophila
Bacillus sp
Body resistance
Common carp
Cyprinus carpio
Opis:
This study aims to determine the best density of Bacillus sp. which is given through feed to increase the body resistance of carp that is infected by pathogenic bacteria, Aeromonas hydrophila. The research was conducted at the Aquaculture Laboratory and Laboratory of Microbiology and Biotechnology at FPIK UNPAD. This study used an experimental method with a completely randomized design consisting of 4 treatments and 3 replications, namely treatment A (control), treatment B (Bacillus sp. 106 cfu/mL feed), treatment C (Bacillus sp. 108 cfu/mL feed) and treatment D (Bacillus sp. 1010 cfu/mL feed) for two weeks later in Aeromonas hydrophila infection with an intramuscular density of 108 cfu/mL for seven days. Observation parameters were clinical symptoms, fish response to feed, fish response to shock and survival. The results of this study showed that treatment C (Bacillus sp. 108 cfu/mL feed) resulted in 89% survival and reduced clinical symptoms.
Źródło:
World Scientific News; 2019, 133; 263-274
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biological degradation of metribuzin and profenofos by some efficient bacterial isolates
Autorzy:
Tamilselvan, C.
Joseph, S.J.
Mugunthan, G.
Sathish Kumar, A.
Syed Musthaq Ahamed, S.
Powiązania:
https://bibliotekanauki.pl/articles/11692.pdf
Data publikacji:
2014
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
biological degradation
metribuzin
profenofos
bacterial isolate
Pseudomonas aeruginosa
Staphylococcus aureus
Bacillus subtilis
Opis:
The soil sample was collected from the paddy field of Sriperumbudur, Tamilnadu which is having a history of repeated pesticide applications. The isolation of efficient pesticide degrading bacteria was identified as Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis. The growth of the three pesticide degrading isolates was assessed in Minimal salt broth containing 25 ppm of pesticides. Two popularly used pesticides Metribuzin and Profenofos were selected for this study. Among the three bacterial isolates, the bacteria Bacillus subtilis utilized the pesticides effectively and showed maximum growth. The growth of the three pesticides degrading isolates Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis was assessed in Minimal salt broth containing 25 ppm of pesticides at different temperature levels (25 °C, 30 °C, 35 °C, 40 °C, 45 °C, 50 °C & 55 °C) and pH levels (pH 4, pH 5, pH 6, pH 7 & pH 8) and carbon sources (Lactose, Dextrose, Fructose, Mannose & Galactose) and nitrogen sources Peptone, Yeast extract, Beef extract, Malt extract and Casein respectively. The maximum growth rate of bacteria was recorded at 35 °C and pH 6. The maximum growth of bacteria was in the presence of Dextrose followed by Fructose, Galactose and Mannose. The least growth was recorded in Lactose broth culture. The maximum growth of bacteria was in the presence of Malt extract followed by Peptone, Yeast extract and Casein. The least growth was recorded in Beef extract broth culture. The bacterial isolates showed maximum growth in the Minimal salt broth containing Profenofos followed by Metribuzin.
Źródło:
International Letters of Natural Sciences; 2014, 09
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isolation and identification of elite phosphate solubilizing bacteria from soil under paddy cultivation
Autorzy:
Gandhi, A.
Muralidharan, G.
Sudhakar, E.
Powiązania:
https://bibliotekanauki.pl/articles/11092.pdf
Data publikacji:
2014
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
isolation
identification
phosphate
phosphate solubilizing bacteria
soil
paddy
plant cultivation
Bacillus
rhizobacteria
Opis:
A considerable number of bacterial species are able to exert a beneficial effect upon plant growth. Mostly they are associated with the plant rhizosphere, so they are called as rhizobacteria. Phosphorus is an essential element for plant development and growth making up about 0.2 % of plant dry weight. Several scientists have reported the ability of different bacterial species to solubilize insoluble inorganic phosphate compounds, such as tricalcium phosphate, dicalcium phosphate, hydroxyapatite, and rock phosphate. Detection and estimation of the phosphate solublization ability of microorganisms have been possible using plate screening methods. Phosphate solubilizers produce clearing zones around the microbial colonies in growth media. In the present investigation a total number of fifteen phosphate solubilizing bacterial colonies isolated from different paddy soils in Cuddalore district of Tamilnadu, India. The isolated PSB were identified and characterized for effective use in the field. All the PSB isolates were identified as Bacillus species and designated as P with serial number from 1 to 15. Among the fifteen isolates, the PSB isolate P6 showed highest amount of phosphate solubilization. The quantity of available phosphorus estimated in the P6 grown Sperber broth culture medium on 7th day was maximum of 321.7 μg/ml which was the highest value compared to other PSB isolates.
Źródło:
International Letters of Natural Sciences; 2014, 11, 1
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Synthesis of some Novel Halogenated Platinum (II) Complexes of Active Schiff’s Base Ligand Derived from 5-Bromo Isatin and Evaluation of their Antibacterial Activity
Autorzy:
Patange, Ashok N.
Yadav, Uttam M.
Desai, Pratik A.
Singare, Pravin U.
Powiązania:
https://bibliotekanauki.pl/articles/1194025.pdf
Data publikacji:
2015
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
5-bromo Isatin
Bacillus pumilus
E. Coli
Micrococcus Lutes
Salmonella abony
Schiff’s base
Semicarbazone
Thiosemicarbazone
Opis:
This paper presents the synthesis and antibacterial studies some novel halogenated Platinum (II) complexes of active Schiff’s base ligands of the type [Pt(L1H)2]Cl2, [Pt(L2H)2]Cl2 and[Pt(L)2] derived from 5-Bromo Isatin. Where L1H= hydrazinecarbothiamide of 5-bromoindolinedione and L2H = hydrazinecarboxamide of 5-bromoindolinedione. All complexes reported here had been characterised by 1H NMR and IR spectral studies. Molar conductance studies support the 1:2 natures of these complexes. Pt (II) complexes are diamagnetic in nature and showing square planner geometry. The IR spectral data reveals that both the Schiff’s bases (L1H and L2H) behave as bidentate ligands and are co-ordinated to Pt (II) metal through the sulfur and hydrogenic nitrogen atom.all the new synthesized compounds were screened for antibacterial activity against four of the test organisms like Bacillus pumilus, Micrococcus Lutes, E. coli and Salmonella abony
Źródło:
World Scientific News; 2015, 10; 32-43
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Microbiological safety of fruit juices consumed in cafes and restaurants of Debre Markos town, north western Ethiopia
Autorzy:
Geta, Kindu
Kebede, Ameha
Chemedissa, Meseret
Powiązania:
https://bibliotekanauki.pl/articles/1076778.pdf
Data publikacji:
2019
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Aspergillus niger
Bacillus cereus
Debre Markos
Enterobacter
Enterococcous
Escherichia coli
Fruit juice
Hygiene
Klebsiella
Microbial safety
Penicillium
Salmonella
Serratia
Shigella
Staphylococcus aureus
Streptococcus
Opis:
Improperly prepared fresh fruit and vegetable juices are recognized as an emerging cause of food borne illnesses. Therefore, this study was aimed at evaluating the microbiological safety of fresh fruit juices marketed in Debre Markos town and their hygienic conditions of preparations. Thirty-six fruit juices samples were collected from 6 cafés and restaurants of Debre Markos town and analyzed for total aerobic viable bacterial count (TAVBC), total staphylococcal count (TSC), aerobic spore forming bacterial count (ASFBC), total coliform count (TCC), fecal coliform count (FCC), yeast and mould count (YMC). The spread plate method was used for the isolation of microorganisms on appropriate selective media. All isolates were characterized following standard methods. Bacterial and fungal species were isolated following standard methods, while questionnaires were distributed to 30 juice makers to obtain preliminary information on their hygienic and safety practices. Results show that the mean TAVBC, ASFBC, TSC, yeast and mould, TCC and FCC of mango were 2.2±0.48×106, 0.13±0.04×105, 0.004×105, 1.1±0.2×106, 0.15±0.05×105, 5.7±3.73×104 and 0.06±0.04×104, cfu/ml, respectively. Moreover, the mean of TAVBC, ASFBC, TSC, YMC, TCC, and FCC of avocado juice were 3.6±0.6×106, 0.08±0.02×105, 0.27±0.07×105, 1.2±0.4×106, 0.02±0.01×105, 6.46±3.7×104, and 0.2±0.1×104 cfu/ml, respectively. The bacterial isolates were identified as S. aureus, E. coli, Klebsiella spp. B. cereus, Enterobacter spp., Enterococcous spp., Streptococcus spp., and Serratia spp., while the identities of the fungal isolates were Fusarium spp., Mucor spp. and S. cerevisiae. The results also showed that the microbial loads of most of the fruit juices were higher than the specifications set for fruit juices sold in the Gulf region and other parts of the world. Most venders obtained fruit from the open market and all juice makers lacked special training in food hygiene and safety. Therefore, regular training and health education on food hygiene and safety is recommended for juice handlers to improve the quality of fresh fruit juices in the study area.
Źródło:
World News of Natural Sciences; 2019, 24; 287-298
2543-5426
Pojawia się w:
World News of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Integrated microsystem for multiplexed genosensor detection of biowarfare agents
Autorzy:
Ema, Sagbor Grinity
Mwadwo, Kwumasi Sylvester
Paul, Bosu P.
Powiązania:
https://bibliotekanauki.pl/articles/1119284.pdf
Data publikacji:
2016
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Bacillus anthracis
Bacteriophage lambda
Brucella melitensis
Burkholderia mallei
Coxiella burnetii
Electrochemical DNA biosensor. Biowarfare agent
Francisella tularensis
Multiplex detection
Self-assembled monolayer (SAM)
Yersinia pestis
Opis:
An early, rapid and definite detection for the presence of biowarfare agents, pathogens, viruses and toxins is required due to their harmful effect to human population. Those potentially encountering the aforementioned include people involved in civil rescue and security, homeland security, military operations, as well as public transportation securities such as airports, metro and railway stations. This work informs the reader of an electrochemical genosensor with an integrated microsystem array combined with microtube fluidics that allows simultaneous detection of different biowarfare agents such as Bacillus anthracis, Brucella melitensis, Bacteriophage lambda, Francisella tularensis, Burkholderia mallei, Coxiella burnetii, Yersinia pestis, and Bacillus thuringiensis var. kurstaki. The chip electrode arrays were modified via coimmobilisation of a 1:100 (mol/mol) mixture of a thiolated probe and a polyethyleneglycol terminated bipodal thiol. Herein, PCR products from relevant biowarfare agents were detected reproducibly through a sandwich assay format with the target hybridised between a surface immobilised probe into the electrode and a horseradish peroxidase-labelled secondary reporter probe, which provided an enzyme based electrochemical signal. Cross-reactivity studies over potential interfering DNA sequences have demonstrated high selectivity using the developed platform producing high-throughput.
Źródło:
World News of Natural Sciences; 2016, 5; 20-32
2543-5426
Pojawia się w:
World News of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-12 z 12

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