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Wyszukujesz frazę "Aspergillus flavus" wg kryterium: Temat


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Tytuł:
Assessment of Total Aflatoxins Level of Two Major Nuts Consumed in Gboko Benue State, Nigeria
Autorzy:
Tor, P. N.
Yande, J. T.
Ijuo, G. A.
Oloruntoba, S. O.
Powiązania:
https://bibliotekanauki.pl/articles/1030152.pdf
Data publikacji:
2020
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Anacardium occidentale
Arachis hypogaea
Aspergillus flavus
Aspergillus nomius
Aspergillus parasiticus
Cashew Nuts
Fungal / Bacteria Counts Total Aflatoxins
Groundnuts
Opis:
The aim of this study was to assess the total aflatoxin (TAT) contents of roasted, dehulled and hulled groundnuts (Arachis hypogaea L.) and of roasted cashew nuts (Anacardium occidentale), sold and consumed in some public areas of Gboko metropolis of Benue State, Nigeria. The items were sampled from 5 different locations based on human traffic densities and sellers of the product and then analysed for TAT contents using direct competitive Enzyme-linked Immunosorbent Assay (ELISA) method. In the roasted cashew nuts, TAT was only detected in samples collected at two of the sampling sites; Mechanic Site/Adekaa (>20.00 µg/kg) and Gboko Main Market (0.30 µg/kg) but not detected in samples collected at the three others (Gboko motor park, Abagu and Tipper Garage). Nevertheless, the detected levels at Gboko Main Market were below the maximum tolerable limits (MTL) of 4 µg/kg set by EU and 10.0 µg/kg set by CAC and NAFDAC, Nigeria for ‘ready to eat’ food substances unlike the situation at the Mechanic Site/Adekaa sampling location. In the roasted groundnuts, levels of TAT higher than the indicated MTL were documented in samples collected at Tipper Garage (12.20 µg/kg) and Abagu (5.70 µg/kg) only exceeded the EU MTL, while the results at Gboko motor parks (2.00 µg/kg), Gboko main market (1.30 µg/kg) and Mechanic site/Adekaa (0.50 µg/kg) were below the EU MTL. Samples of hulled groundnuts collected at Gboko main market recorded TAT levels of 0.10 µg/kg while at Mechanic site/Adekaa it was not detected. Dehulled groundnuts sampled at Gboko Main market (0.40 µg/kg) and Mechanic site/Adekaa (0.20 µg/kg) recorded TAT levels within CAC and NAFDAC MTLs. The dehulled groundnuts samples collected at the Tipper Garage sample location, which recorded a moisture level of 4.20 %; yet among the rest of all the other samples had moisture levels below the recommended maximum tolerable limits of 5.80 % for stored groundnuts and cashew nuts. The results of microbial counts revealed that total mesophilic bacteria ranged between < 1.0 × 101 to >1.0 × 102 CFU/L, fungi varied between < 1.0 ×101 to > 1.0 × 101 CFU/L while E.coli counts varied between 0.00 to 0.32 CFU/L. The microbial contagion was generally low. Nevertheless, prolonged feasting on the aflatoxin-contaminated nuts is possible in the study area due to the rising food insecurity of majority of the masses. The chronic and acute exposures therefore, presents health concern of aflatoxicosis.
Źródło:
World Scientific News; 2020, 143; 170-180
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biocatalytic ketone reduction - a study on screening and effect of culture conditions on the reduction of selected ketones
Autorzy:
Kuncham, R.
Gurumurthy, K.T.
Chandan, N.
Javed, A.
Ashwini, L.S.
Shenoi, R.
Baranwal, A.
Powiązania:
https://bibliotekanauki.pl/articles/11870.pdf
Data publikacji:
2014
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
biocatalysis
ketone reduction
screening
culture condition
Aspergillus ochraceus
Aspergillus flavus
Aspergillus tubingenesis
Aspergillus niger
Rhizopus stolonifer
baker's yeast
enzyme activity
enzyme expression
bioconversion
Opis:
Microbial conversions are gaining importance in the synthesis of important drug metabolites and their intermediates as they are good alternative to chemical synthesis since they are enantio-selective and regio-selective and even can be carried out at ambient temperature and atmospheric pressure. Till date, biocatalytic reduction of acetophenone and its derivatives has been widely reported. In the present study, we have made an attempt to carry out the microbial bioreduction of o-hydroxyacetophenone by screening some of the selected microorganisms which were obtained from culture collection centre as well as those which are isolated in our Microbiology lab. The selected microorganisms include Aspergillus ochraceous, Aspergillus flavus, Aspergillus tubingenesis, Aspergillus niger, Rhizopus stolanifer MTCC 162, Rhizopus stolanifer MTCC 2591 and Baker’s yeast.Among the seven microorganisms screened for the bioreduction of o-hydroxyacetophenone, Baker’s yeast and Aspergillus tubingenesis showed significant bioconversion where as Aspergillus ochraceous exhibited the least bioconversion.In our earlier study it was found that Aspergillus flavus has the required bioreductase enzyme, which showed the maximum conversion of p-chloroacetophenone to p-chlorophenylethanol. Hence optimization of culture conditions to get maximum enzyme expression and hence maximum conversion was thought off. The parameters considered for the study include effect of various Carbon sources, Nitrogen source, Metal ions, incubation Temperature and media pH on enzyme expression. The optimized culture a condition at which maximum bioconversion was achieved was maltose among various carbon sources. Tryptone was found to have maximum effect among the nitrogen sources. Media pH 7.6 and incubation temperature of 35 °C was found to be favourable for maximum enzyme activity. Among various divalent metal salts, addition of magnesium sulphate to the media significantly increased the enzyme activity.
Źródło:
International Letters of Natural Sciences; 2014, 01
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-2 z 2

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