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Wyświetlanie 1-2 z 2
Tytuł:
The multiple HPV infections and their possible significance in predicting the risk of the cervical cancer
Autorzy:
Kożańska, Aleksandra
Pisarska, Justyna
Baldy-Chudzik, Katarzyna
Powiązania:
https://bibliotekanauki.pl/articles/1070878.pdf
Data publikacji:
2019
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
HPV co-infection
PCR-RFLP
Real-Time PCR
cervical cancer
human papillomavirus
Opis:
Infections with human papillomavirus (HPV) are detected frequently. The occurrence of persistent infection with oncogenic HPV type associates with the development of cervical cancer. However, the part of HPV infections consist of the multiple viral types. The influence of HPV co-infections on the risk of the development of cervical cancer is not clear, but the occurrence of more than one viral type seems to increase the risk of advanced lesions of the uterine cervix. Additionally, possible interactions (both positive and negative) between particular HPV types in co-infections may influence the risk of the lesions progression. Although, the issue of HPV co-infections is not well known, the previous scientific reports make that the introduction of the molecular methods, allowing the identification not only single but also multiple HPV infections, in routine diagnostics seem to be important. Here, we present the PCR-RFLP and Real-Time PCR methods as the useful tools in the diagnostics of HPV infections.
Źródło:
World Scientific News; 2019, 123; 192-207
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Detection of C677T & A1298C mutations within the MTHFR gene by PCR and RFLP assays and assessment of risk factor of Hyperhomocysteinemia
Autorzy:
Amarakoon, A. A. D. Gayathri Upeksha
Fernandopulle, Neil
Powiązania:
https://bibliotekanauki.pl/articles/1182887.pdf
Data publikacji:
2016
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
mthfr gene
dna methylation
hyperhomocysteinemia
Opis:
The MTHFR gene within the human genome, codes for the synthesis of Methylenetetrahydrofolate Reductase enzyme, which reduces 5,10-Methylenetetrahydrofolate to 5-Methyltetrahydrofolate, which in turn, is the major circulatory form of folate in the blood. Folate, in this form, among it’s other functions, is involved in reducing the homocysteine levels in the blood, whose elevated levels lead to Hyperhomocysteinemia, causing various major disorders. Mutations within the gene lead to impairment of gene function, in turn causing the homocysteine levels to rise. The C677T and A1298C mutations are the main causative agents for MTHFR gene disruption. During the course of the project, a total of 79 samples were analyzed for the presence of these mutations. The blood samples were first subjected to PCR, giving two separate DNA fragments each responsible for either of the conditions. The fragments were then subjected to RFLP analysis to detect the mutations. The results were finally given with respect to the risk factor faced by each individual based on a molecular diagnostic point of view.
Źródło:
World Scientific News; 2016, 53, 3; 253-274
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-2 z 2

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