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    Wyświetlanie 1-10 z 10
    Tytuł:
    Proteomics of E.coli Nissle 1917 in response to Cocos nucifera sap and wine
    Autorzy:
    Chandrasekhar, K
    Pramoda Kumari, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/11862.pdf
    Data publikacji:
    2015
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    proteomics
    Escherichia coli
    plant response
    coconut palm
    Cocos nucifera
    sap
    wine
    protein
    probiotic
    identification
    scanning
    Opis:
    In the present study, we described the protein profile experimentally by 2D-PAGE and MALDI analysis to understand the stress mechanisms of cocoti sap and wine on E.coli Nissle 1917. We isolated one newly expressed protein from cocoti wine treated gel which is not present in both control and cocoti sap treated sample i.e. P21 prophage-derived head-stabilizing proteinVG03_ECOL6 (3n1) also called as Head protein gp3. This protein mainly activities related to the viral life cycle. It helps to attach the viral gene into host. The growth rate was delayed in cocoti wine treated E.coli Nissle 1917 when compared to control and cocoti sap treated samples. Stress mechanism induce many proteins they are involved in metabolic process, hydrolase activity, lyase activity, quinone binding, phosphotransferase system, carbohydrate metabolism, DNA binding, DNA repair, transferase activity, oxidoreductase, purine metabolism, transcription antitermination, transcription regulation and other related activities. We proved that the predicted protein structure quality, resolution, density and error plot values by QMEAN analysis. Based on these results, only two differentially expressed proteins under sap stress showed that the significant results, which were N-acetylgalactosamine-specific phosphotransferase enzyme IIB component 1, PTPB1_ECOLI and DinI-like protein Z3305/ECs2939 in prophage CP-933VDINI1_ECO57. In case of wine stress, the differentially expressed proteins were Transcription anti-termination protein RFAH- ECO57 NusA and PUR7- eco24- phosphoribosylamidazole-succinocarboxamide synthase showed significant results. ProtParam analysis indicating that the multiple physico-chemical characters of differentially expressed proteins were differed and compared. The phylogenetic tree represents the relationship in-between the differentially expressed proteins, were showed siblings (related) as well as monophytic clade.
    Źródło:
    International Letters of Natural Sciences; 2015, 41
    2300-9675
    Pojawia się w:
    International Letters of Natural Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Studies on biosynthesis of silver nanoparticles using Rhizopus sp. and its antibacterial efficacy on E. coli MDR strains
    Autorzy:
    Hiremath, J.
    Rathod, V.
    Ninganagouda, S.
    Singh, D.
    Prema, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/11479.pdf
    Data publikacji:
    2014
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    biosynthesis
    silver nanoparticle
    Rhizopus
    antibacterial activity
    Escherichia coli
    multidrug resistance
    nanotechnology
    Opis:
    Nanotechnology is a field that is burgeoning day by day, making an impact in all spheres of human life. Biological methods of synthesis have paved way for the “greener synthesis” of nanoparticles and these have proven to be better methods due to slower kinetics, they offer better manipulation and control over crystal growth and their stabilization. In this context we have investigated extracellular biosynthesis of silver nanoparticles (AgNPs) using cell-free extract of Rhizopus spp.. Formation of AgNPs was indicated by the change in the colour of the cellfree extract from yellow to dark brown under static condition after 48 hrs of incubation. Characterization of AgNPs was carried out by UV-Vis Spectroscopy which gave sharp plasmon resonance peak at 429 nm corresponding to spherical shaped nanoparticles. Transmission electron microscopy (TEM) micrograph showed formation of well-dispersed AgNPs in the range of 25-50 nm. Scanning electron microscopy (SEM) showed the particles to be uniformly dispersed without agglomeration with smooth morphology. EDS showed the presence of elemental silver at 3kev. X-ray diffraction (XRD)-spectrum of the AgNPs exhibited 2θ¸ values corresponding to nanocrystal. These biosynthesized AgNPs were used to study their antimicrobial activity against Multi-drug resistant (MDR) E. coli strains, by Agar diffusion method. Zone of inhibition was measured. Synthesis of nanosized particles with antibacterial properties, which are called "nanoantibiotics", is of great interest in the development of new pharmaceutical products.
    Źródło:
    International Letters of Natural Sciences; 2014, 18
    2300-9675
    Pojawia się w:
    International Letters of Natural Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Isolation of bacteria from soil sample of Tamil Nadu and their in vitro interaction
    Autorzy:
    Sankari, D.
    Khusro, Ameer
    Aarti, Chirom
    Powiązania:
    https://bibliotekanauki.pl/articles/1031683.pdf
    Data publikacji:
    2020
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Antagonism
    B. subtilis
    CFU
    E. coli
    Interaction
    P. aeruginosa
    Opis:
    The present study was investigated to determine the competitive interaction between Gram positive (B. subtilis) and Gram negative (P. aeruginosa and E. coli) bacteria in vitro. Bacteria of interest were isolated from soil sample and were identified through morphological and biochemical tests. Bacterial cultures were grown alone and as co- culture in test tubes containing sterile broth. Bacterial growth and their growth inhibition in co- culture tubes were analyzed till 96 h through spectrophotometric assay and colony forming unit (CFU) assay. Spectrophotometric analysis and CFU/mL assay showed that the effect of B. subtilis on P. aeruginosa and E. coli in co- culture tubes was growth inhibitory. This is accompanied by the reduction in absorbance value and CFU/mL of the co-culture tubes. The bactericidal activity of B. subtilis was measured on P. aeruginosa and E. coli using agar well diffusion method. B. subtilis showed zone of inhibition of 8 mm and 6 mm against P. aeruginosa and E. coli, respectively. The antagonistic activity test of B. subtilis favoured the spectrophotometric and colony forming assay results. These results suggest that B. subtilis develop adaptational pathways by extracellular signaling molecules and antibacterial factors in order to compete with bacterial neighbors.
    Źródło:
    World News of Natural Sciences; 2020, 28; 67-75
    2543-5426
    Pojawia się w:
    World News of Natural Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Screening for ESβL producing E. coli isolated from clinical urine samples collected from different places of Kalaburagi city
    Autorzy:
    Ramachar, Raghavendra
    Gaddad, Subhashchandra M.
    Shivannavar, Channappa T.
    Powiązania:
    https://bibliotekanauki.pl/articles/1177430.pdf
    Data publikacji:
    2018
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Antibiogram
    Double-disc diffusion test
    ESβL
    Green Metallic Sheen
    Phenotypic detection
    Uropathogenic E. coli
    Opis:
    Bacteria are capable of invading and infecting humans, leading to disease and sometimes death. Different body tissues, organs and systems are vulnerable to different organisms. This study was aimed to isolate and identify the bacteria causing Urinary Tract Infection (UTI) in different age groups and also screened for ESβL production. The mid-stream urine samples showing symptoms of urinary tract infections were collected in a wide container from different hospitals and diagnostic centres of Kalaburagi city. The isolation of uropathogen was done by semi-quantitative method of inoculating the samples on the selective and differential media such as Eosin Methylene Blue (EMB) and MacConkey (MAC) agar media respectively. The isolated pathogen was identified by conventional methods like cultural, morphological and biochemical tests. The antibiotic susceptibility test was carried out by Kirby-Bauer disc diffusion technique and ESβL production by double disk-diffusion test (DDDT) as per CLSI guidelines (2013). Out of 550 samples screened, a total of 288 bacteria were isolated of those half of them (146) were E. coli isolates and all were found to be multidrug resistant and half of them (50%) were of ESβL producers. All ESβL producing E. coli isolates were resistant to ceftazidime and exhibited higher level of resistance to Cephalothin, Erythromycin, Cotrimoxazole and Aztreonam. As ESβL producing organisms limits the available treatment options, so, the timely administration of sensitive antibiotic and avoiding antibiotic abuse will help in curing the disease without going for the costly drugs such as Carbapenems. Our study revealed that nitrofurantoin, ofloxacin and cefoxitin may be considered as drug of choice for the treatment of UTI patients. The ESβL production in uropathogens should be continuously monitored in the clinics and hospitals as to avoid the emergence of more multi drug resistance strains.
    Źródło:
    World Scientific News; 2018, 103; 107-118
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Detection and enumeration of Enteric bacteria associated with food handlers and surfaces of food manufacturing industry located in Hub city, Pakistan
    Autorzy:
    Ali, Razim
    Hayat, Amir
    Fatima, Madiha
    Noman, Muhammad
    Powiązania:
    https://bibliotekanauki.pl/articles/1182928.pdf
    Data publikacji:
    2016
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    enterobacteriaceae
    foodborne microbes
    hygiene
    surface contamination
    food handlers
    e. coli
    sanitation
    Opis:
    Foodborne microorganisms harbor and adheres itself to the food material and surrounding surfaces for a long time and influence the food quality and consumers health. Among these microbes the presence of Enteric indicator bacteria in food premises confers the indication of entero-pathogens, i.e. E. coli, Salmonella, Shigella and Campylobacters that could cause severe systemic infections in consumers. In this study, the hygienic status of confectionery and supplementary food processing facility was evaluated. A total of 10497 examinations were performed on 3499 swab samples collected from food premises and handlers for the analysis of Enteric indicator bacteria. From swabs, 1277 (12.2%) isolates were identified in which Enterobacteriaceae were found with higher frequency 604 (47.3%) followed by Coliforms 293 (30.8%) and Escherichia coli 280 (21.9%) respectively. The mean count (CFU/cm2) was found maximum for plain surfaces (floors, walls and door), while the lowest was for equipment and machinery. Overall isolates percent prevalence was determined where Enterobacteriaceae were 47%, Coliforms 31% and Escherichia coli 22%. Majority of the floor surfaces were highly contaminated, where washing and sanitation practices were observed to be inappropriate. Worker hygiene status was lacking essential food safety and hygiene standards. In general, the Enteric bacteria were found with higher ratio, that could affect the food quality and quantity both to a greater extent with some influences on consumers health.
    Źródło:
    World Scientific News; 2016, 49, 2; 192-203
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Synthesis of some Novel Halogenated Platinum (II) Complexes of Active Schiff’s Base Ligand Derived from 5-Bromo Isatin and Evaluation of their Antibacterial Activity
    Autorzy:
    Patange, Ashok N.
    Yadav, Uttam M.
    Desai, Pratik A.
    Singare, Pravin U.
    Powiązania:
    https://bibliotekanauki.pl/articles/1194025.pdf
    Data publikacji:
    2015
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    5-bromo Isatin
    Bacillus pumilus
    E. Coli
    Micrococcus Lutes
    Salmonella abony
    Schiff’s base
    Semicarbazone
    Thiosemicarbazone
    Opis:
    This paper presents the synthesis and antibacterial studies some novel halogenated Platinum (II) complexes of active Schiff’s base ligands of the type [Pt(L1H)2]Cl2, [Pt(L2H)2]Cl2 and[Pt(L)2] derived from 5-Bromo Isatin. Where L1H= hydrazinecarbothiamide of 5-bromoindolinedione and L2H = hydrazinecarboxamide of 5-bromoindolinedione. All complexes reported here had been characterised by 1H NMR and IR spectral studies. Molar conductance studies support the 1:2 natures of these complexes. Pt (II) complexes are diamagnetic in nature and showing square planner geometry. The IR spectral data reveals that both the Schiff’s bases (L1H and L2H) behave as bidentate ligands and are co-ordinated to Pt (II) metal through the sulfur and hydrogenic nitrogen atom.all the new synthesized compounds were screened for antibacterial activity against four of the test organisms like Bacillus pumilus, Micrococcus Lutes, E. coli and Salmonella abony
    Źródło:
    World Scientific News; 2015, 10; 32-43
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The Effect of Garlic Extract Addition on Tilapia Skin Gelatin Based Edible Coating Towards Antimicrobial Properties and Fish Meatball’s Shelf Life
    Autorzy:
    Reynaldi, Ahmad
    Rostini, Iis
    Afrianto, Eddy
    Rochima, Emma
    Powiązania:
    https://bibliotekanauki.pl/articles/1058047.pdf
    Data publikacji:
    2019
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    E. coli
    Pseudomonas aeruginosa
    Salmonella typhimurium
    Staphyllococcus aureus
    TPC
    edible coating
    extract
    garlic
    pH values
    shelf life
    tilapia gelatin
    Opis:
    The purpose of this research is to determine the best concentration and effect of garlic extract incorporated on tilapia skin gelatin-based edible coating to preserve and extend the shelf life of fish meatball at cold storage. This research was conducted from May 2018 until October 2018 on Fisheries Product Processing Laboratory of The Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran. The garlic extract conducted with maceration method with ethanol 96% was performed at Organic Chemical Laboratory FMIPA Universitas Padjadjaran, and for TPC test, inhibition test of edible coating Tilapia’s fish-skin with garlic extract was conducted in Microbiology and Molecular Biotechnology Laboratory of Faculty of Fisheries and Marine Sciences Universitas Padjadjaran. The method used is experimental method with 5 treatments which is meatballs without edible coating. The addition of garlic extract was done with various concentration at 0%, 1%, 1.5%, 2%. The observations were Total Plate Count (TPC) test, inhibition zone test against Pseudomonas aeruginosa, and pH value. The results showed that the best treatment is 2% garlic extract with TPC value 1.1×105 cfu/g during 14 days of storage and concluded that it could extend the shelf life of meatball until 14 days, pH value was 7.03 and inhibition zone against P. aeruginosa was 7.01 mm. The addition of garlic extract on tilapia skin gelatin-based edible coating could extend the shelf life of fish meatballs until 14 days on cold storage.
    Źródło:
    World Scientific News; 2019, 134, 2; 74-85
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Antibiotic Susceptibility Test of Bacteria Isolated From Fruit Juices Sold in Cafes and Restaurants of Debre-Markos Town, North Western Ethiopia
    Autorzy:
    Geta, Kindu
    Kebede, Ameha
    Chemedissa, Meseret
    Powiązania:
    https://bibliotekanauki.pl/articles/1076786.pdf
    Data publikacji:
    2019
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Antibiotics
    Antimicrobial resistance
    B. cereus
    Debre Markos
    E. coli
    Enterobacter
    Fruit juices
    Klebsiella
    Pathogens
    Ps. aeruginosa
    Salmonella
    Shigella
    Staphylococcus aureus
    Opis:
    The prevalence of antimicrobial resistance among food pathogens has increased during recent decades. In this work, pathogenic bacteria such as Staphylococcus aureus, Salmonella spp., Shigella spp., and E. coli were isolated following standard methods. The bacterial isolates were then tested for their sensitivity to common antibiotics using the disc diffusion method on Mueller-Hinton Agar. All of the pathogenic bacteria were found to be resistant to erythromycin and almost all were sensitive to penicillin.
    Źródło:
    World News of Natural Sciences; 2019, 24; 365-371
    2543-5426
    Pojawia się w:
    World News of Natural Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Novel synthesis of 3-(((E)-benzylidene)amino)-5-((Z)-3,4-dimethoxybenzylidene)-2-phenyl-3,5-dihydro-4H-imidazol-4-one derivatives as potent antimicrobial agents
    Autorzy:
    Manawar, Rohit B.
    Parmar, Mukesh B.
    Nayaka, Indresh J.
    Pandit, Brinda H.
    Shah, Manish K.
    Powiązania:
    https://bibliotekanauki.pl/articles/1066261.pdf
    Data publikacji:
    2019
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    4H-imidazol-4-one
    A. niger
    Anti-fungal
    Anti-microbial Agent
    Bioactivity
    C. albicans
    E. coli
    Elemental analysis
    IR spectroscopy
    Mass spectroscopy
    NMR spectroscopy
    P. aeruginosa
    S. aureus
    S. pyogenes
    Opis:
    Novel synthesis of 4H-imidazol-4-one derivative by reaction of (Z)-3-amino-5-(3,4 dimethoxybenzylidene)-2-phenyl-3,5-dihydro-4H-imidazol-4-one with derivative of different aromatic aldehydes and Salicyldehyde. New synthesized compound are thermally stable and neither air-nor moisture sensitive at RT. Successive library of synthesized compound characterized using IR, NMR, Elemental, Mass, and its antimicrobial activity study.
    Źródło:
    World Scientific News; 2019, 130; 42-70
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cloning of a Rift Valley Fever fusion gene in a plant virus derived replicon vector
    Autorzy:
    Omosimua, Rebecca Oziohu
    Iyappan, Gowtham
    Obembe, Olawole
    Ogunkanmi, Adebayo
    Sathishkumar, Ramalingam
    Powiązania:
    https://bibliotekanauki.pl/articles/1193478.pdf
    Data publikacji:
    2021
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    DNA Cloning
    Escherichia coli DH5α cells
    PBYR2e3K2Mc-GFP
    PBYR2e3K2Mc-RVFV
    RVF disease
    RVFV fusion gene
    bean yellow dwarf derived vector
    emerging disease
    geminivirus vector
    zoonotic disease
    Opis:
    Rift Valley fever (RVF) disease is an emerging viral zoonotic disease caused by Rift Valley fever virus (RVFV). RVF disease is seemingly becoming more severe in endemic populations and in areas of new outbreak. RVF disease is listed by WHO as requiring urgent research and development attention. There is no therapeutics or licensed vaccine for human use in the case of an eventual outbreak. There is a need to clone RVFV genes in cloning vectors which may be useful to produce antigens in plant cells or may be tested directly as DNA vaccines. A synthesized Rift Valley fever virus fusion gene was cloned in a bean yellow dwarf virus derived replicon vector; PBYR2e3K2Mc-GFP by removing and replacing the GFP gene. The cloned PBYR2e3K2Mc-RVFV fusion gene was confirmed by PCR, restriction digestion and DNA sequencing. This cloned RVFV fusion gene in plant virus vector can be used for subsequent protein expression in plants cells or used directly tested as DNA vaccines in future studies.
    Źródło:
    World Scientific News; 2021, 158; 159-172
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-10 z 10

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