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Wyświetlanie 1-13 z 13
Tytuł:
Opoznione rozpoznanie wlosnicy w ognisku na terenie Wielkopolski
Autorzy:
Stefaniak, J
Kacprzak, E.
Powiązania:
https://bibliotekanauki.pl/articles/841450.pdf
Data publikacji:
1997
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Wielkopolska
wlosnica
choroby pasozytnicze
choroby czlowieka
parazytologia
Opis:
The study compares chronologically the weeks in which Trichinella spiralis infected meat was consumed. The first symptoms appeared and clinical diagnosis of trichinellosis was made in 37 patients. Several factors were responsible for the delay in final diagnosis of trichinellosis: widely distributed infected meat, contemporary common respiratory viral infections in population with fever and myalgia, diagnostic difficulties in the first index case.
Źródło:
Annals of Parasitology; 1997, 43, 1; 89-91
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Opóźnione rozpoznanie włośnicy w ognisku na terenie Wielkopolski
DELAYED DIAGNOSIS OF TRICHINELLOSIS IN A FOCUS IN POZNAŃ PROVINCE, POLAND
Autorzy:
Stefaniak, J.
Kacprzak, E.
Powiązania:
https://bibliotekanauki.pl/articles/2148894.pdf
Data publikacji:
1997
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Wielkopolska
wlosnica
choroby pasozytnicze
choroby czlowieka
parazytologia
Opis:
The study compares chronologically the weeks in which Trichinella spiralis infected meat was consumed. The first symptoms appeared and clinical diagnosis of trichinellosis was made in 37 patients. Several factors were responsible for the delay in final diagnosis of trichinellosis: widely distributed infected meat, contemporary common respiratory viral infections in population with fever and myalgia, diagnostic difficulties in the first index case.
Źródło:
Wiadomości Parazytologiczne; 1997, 43, 1; 89-91
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wystepowanie gatunkow i genotypow Giardia u ludzi i zwierzat w Wielkopolsce
Occurrence of Giardia species and genotypes in humans and animals in Wielkopolska region, Poland
Autorzy:
Solarczyk, P.
Powiązania:
https://bibliotekanauki.pl/articles/837534.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Wielkopolska
wystepowanie
pasozyty jelitowe
pasozyty zwierzat
genotyp
Giardia
pierwotniaki
gatunki
pasozyty czlowieka
Źródło:
Annals of Parasitology; 2009, 55, 4; 459-462
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Serological screening for congenital toxoplasmosis in newborns based on filter-paper blood cards
Autorzy:
Paul, M.
Petersen, E.
Pawlowski, Z.
Szczapa, J.
Powiązania:
https://bibliotekanauki.pl/articles/840057.pdf
Data publikacji:
1998
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
toxoplasmosis
congenital toxoplasmosis
Toxoplasma gondii
serological screening
screening programme
Wielkopolska region
antibody
Źródło:
Annals of Parasitology; 1998, 44, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Występowanie gatunków i genotypów Giardia u ludzi i zwierząt w Wielkopolsce
Occurrence of Giardia species and genotypes in humans and animals in Wielkopolska region, Poland
Autorzy:
Solarczyk, P.
Powiązania:
https://bibliotekanauki.pl/articles/2143568.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Wielkopolska
wystepowanie
pasozyty jelitowe
pasozyty zwierzat
genotyp
Giardia
pierwotniaki
gatunki
pasozyty czlowieka
Opis:
Giardia is the most common intestinal protozoan parasite found in humans and animals worldwide. Although it has been known for three hundred years, the nomenclature, taxonomy, host specificity, and pathogenicity of Giardia still arouse numerous controversies and ambiguities. Giardia is classified into six species, that are characterised by various ranges of hosts. The most dubious species is G. intestinalis, which includes a dozen or so genotypes, and only two of them (genotype A and B) have wide ranges of hosts, including humans. Moreover, in some genotype assemblages of G. intestinalis certain subgenotypes were distinguished and it was proven that in the same host species various subgenotypes of this parasite may occur. Bearing in mind the significant genetic heterogeneity of G. intestinalis and the fact that various genotypes and subgenotypes of this parasite are characterised by the broad or narrow host specificity, the data concerning the frequency of giardiosis occurrence are insufficient. It is necessary to use molecular biology techniques in order to define the genotype and/or the subgenotype of G. intestinalis that are found in humans and in certain animal species. Furthermore, since more and more pieces of evidence connected with a possibility of the sexual recombination of Giardia are gathered, it is unknown if genotypes and subgenotypes of this parasite are stable in time. The aim of this thesis was to define the frequency of Giardia occurrence in humans and animals in Wielkopolska region, to identify species and genotypes of Giardia that occur in humans and animals, as well as to obtain an axenic culture of the chosen isolates of Giardia from animals and to compare the sequence of the b-giardin gene fragment obtained from the DNA isolated from cysts and trophozoites in order to check if the axenisation of G. intestinalis leads to the selection of genotypes or if Giardia genotypes are stable in time. Altogether, 2183 faecal samples were examined for the presence of Giardia cysts; 447 faecal samples were taken from 232 persons coming from 5 cities situated in Wielkopolska, and 1736 faecal samples were obtained from 123 animal species, including: 266 faecal samples from 113 species of animals kept in the Zoological Garden in Poznań, 1286 samples from 4 species of breeding animals, 118 samples from dogs, and 66 samples from 5 species of wild animals. Faecal samples were taken from animals coming from 25 places in Wielkopolska. Moreover, seven isolates of G. intestinalis were used in the studies, which were obtained from various species of hosts and kept in an axenic in vitro culture. Microscopic, molecular and bio-informative methods were used in the studies. From each faecal sample fresh smears were made in a 0.6% solution of physiological salt and in Lugol’s solution, as well as a permanent smear stained with trichrome was made. Moreover, the following molecular techniques were implemented in the studies: DNA extraction and purification, the PCR technique (two molecular markers), electrophoresis and visualisation of PCR products, and sequencing. A fragment of the b-giardin gene was used as a molecular marker in order to define the genotype and subgenotype of Giardia. Only in the case of genotyping of two Giardia isolates obtained from Peromyscus eremicus another molecular marker (SSU rRNA)was additionally used. Some widely available computer programmes (Chromas, CAP 3, BioEdit, BLASTn, MEGA version 4.0) were utilised in the analysis of the sequence of the b-giardin gene fragment and in the phylogenetic analysis. The culture of Giardia trophozoites was established to compare the sequence of the partial b-giardin gene from cysts and trophozoites. Concentration and purification of Giardia cysts in the saccharose gradient, and the excystation technique were applied in the studies to obtaining an axenic in vitro culture. In this study, Giardia cysts were found in 12 faecal samples obtained from 3 persons and 5 animal species. Giardia cysts were found only in faecal samples from humans living in Poznań and the samples obtained from animals coming from Poznań and around Puszczykowo. The highest frequency of infection was stated in domestic animals (2.5%) and in animals kept in the Zoological Garden (2.0%), whereas a slightly lower frequency was noticed in wild animals (1.5%) and in humans (1.3%). No Giardia cysts were found in the faecal samples collected from breeding animals. Two new species of Giardia hosts were identified, namely Rhinella marina and Peromyscus eremicus; however, due to a minimal amount of faecal samples supplied for the study it was impossible to define the species and genotype of this parasite. PCR products (the partial of b-giardin gene) were obtained in seven faecal samples out of the ten studied, including three samples from people and four faecal samples derived from three animal species (i.e. dog, tamandua, red deer). Moreover, molecular characterization of seven Giardia isolates from three persons and four animal species (red-bellied monkey, silver marmoset, Thomson’s gazelle, and sheep) kept in an axenic in vitro culture was performed. Based on the b-giardin sequence fragment analysis, four assemblages of G. intestinalis genotypes were identified (A, B, C and D). In humans, A and B G. intestinalis genotypes and three subgenotypes, including a cosmopolitan subgenotype A2 and two new subgenotypes A and B were detected. Furthermore, four G. intestinalis genotypes were found in animals, including three genotypes which are non-infectious to humans, namely: genotypes C and D in dogs and a cervids-specific genotype A in red deer (Cervus elaphus), which indicate that these animals do not constitute the source of infection to humans. On the other hand, in a tamandua from the Zoological Garden in Poznań a new subgenotype B of G. intestinalis was identified, which due to a close relationship with Giardia isolates obtained from humans is potentially infectious to man. In none of the studied faecal samples a mixed infection of Giardia was found. To date, nine sequences of the partial b-giardin gene have been deposited in the National Center for Biotechnology Information (NCBI), including two sequences of Giardia isolates obtained from humans (GenBank accession numbers FJ009207, FJ009208), three sequences of isolate obtained from red deer (GenBank accession numbers EU621373, EU626198, EU216429), two sequences of both Giardia isolates obtained from dogs (GenBank accession numbers FJ009205, FJ009206), and the single sequences obtained from tamandua (GenBank accession number FJ009209) and from Thomson’s gazelle (GenBank accession number EU626199). According to the literature, an axenic in vitro culture of G. intestinalis was obtained from a red deer for the first time. Based on the analysis of the sequence of the b-giardin gene fragment obtained from the DNA isolated from cysts and trophozoites it was proven that the red deer was infected with a single population of Giardia and that during the axenisation of the culture no mutation in the DNA of the parasite’s trophozoites took place. Probably the time distance that the DNA was isolated from the trophozoites kept in the culture was too short to cause the mutation. This suggestion is confirmed by the results of the genotyping of seven G. intestinalis isolates obtained from various host species and kept in an axenic in vitro culture for at least a number of years. Based on the molecular characteristics it was stated that all the studied isolates from the axenic culture were identical and belonged to the same assemblage B. The comparision with the sequences from GenBank database revealed that all mentioned isolates were 99% similar to the sequence of Giardia Nij5 isolate obtained from a person from the Netherlands and characterised as genotype B1. Due to the sameness of the molecular marker sequences it seems improbable that the identical G. intestinalis genotype occurred in various time periods (the largest difference was 14 years) in humans and in a number of animal species in diverse areas of Wielkopolska region. Quite opposite, the long-term keeping of these isolates in the homogenous conditions of an axenic in vitro culture leads to the selection of a genotype or proves the instability of genotypes of this parasite. Long-term studies need to be conducted in order to verify these hypothesis. Their results will have a key meaning in explaining the genetic structure of the Giardia population and in understanding the molecular epidemiology of giardiosis.
Źródło:
Wiadomości Parazytologiczne; 2009, 55, 4; 459-462
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Detection of Trichinella sp.invasion and clinical appraisal of patients in the late stage of trichinellosis in a new epidemic focus in Wielkopolska
Autorzy:
Kociecka, W
Pielok, L.
Pietrzak, H.
Gustowska, L.
Powiązania:
https://bibliotekanauki.pl/articles/837410.pdf
Data publikacji:
1997
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
patient
Trichinella
diagnosis
trichinellosis
clinical analysis
clinical appraisal
invasion
epidemiological analysis
Wielkopolska region
Opis:
A clinical and an epidemiological analysis was presented of a trichinellosis focus from Wielkopolska region. The studies included 20 persons and trichinellosis was diagnosed in 15 cases. The focus was characterized by asynchronous invasion with Trichinella sp. in individual affected persons in the course of a prolonged period (around 2.5 months) and by reporting of the patients to the Clinic during approximately 30 to 90 days after the disease signs/symptoms developed. Retrospective analysis and a set of specialized tests permitted to clarify causes of persisting complaints, presented by the patients, as well as to establish the diagnosis at late stages of trichinellosis.
Źródło:
Annals of Parasitology; 1997, 43, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Detection of Trichinella sp.invasion and clinical appraisal of patients in the late stage of trichinellosis in a new epidemic focus in Wielkopolska
Autorzy:
Kocięcka, W.
Pielok, L.
Pietrzak, H.
Gustowska, L.
Powiązania:
https://bibliotekanauki.pl/articles/2148899.pdf
Data publikacji:
1997
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
patient
Trichinella
diagnosis
trichinellosis
clinical analysis
clinical appraisal
invasion
epidemiological analysis
Wielkopolska region
Opis:
A clinical and an epidemiological analysis was presented of a trichinellosis focus from Wielkopolska region. The studies included 20 persons and trichinellosis was diagnosed in 15 cases. The focus was characterized by asynchronous invasion with Trichinella sp. in individual affected persons in the course of a prolonged period (around 2.5 months) and by reporting of the patients to the Clinic during approximately 30 to 90 days after the disease signs/symptoms developed. Retrospective analysis and a set of specialized tests permitted to clarify causes of persisting complaints, presented by the patients, as well as to establish the diagnosis at late stages of trichinellosis.
Źródło:
Wiadomości Parazytologiczne; 1997, 43, 3; 257-263
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prevalence of cryptosporidiosis in livestock in Wielkopolska Region
Autorzy:
Majewska, A.C.
Werner, A.
Sulima, P.
Powiązania:
https://bibliotekanauki.pl/articles/840450.pdf
Data publikacji:
1998
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
prevalence
cryptosporidiosis
Polska
economic loss
cryptosporidial infection
farm animal
Cryptosporidium
Wielkopolska region
livestock
Źródło:
Annals of Parasitology; 1998, 44, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Trichinellosis among wild mammals in Wielkopolska Region
Autorzy:
Nowosad, P.
Powiązania:
https://bibliotekanauki.pl/articles/840080.pdf
Data publikacji:
1997
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
parasitic disease
Trichinella
man
mammal
Polska
trichinellosis
Trichinella britovi
Wielkopolska region
wild mammal
domestic animal
Źródło:
Annals of Parasitology; 1997, 43, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Porównanie skażenia gleby jajami Toxocara canis i Toxocara cati w środowisku wiejskim i miejskim w Wielkopolsce w latach 2000-2005
A comparison of soil contamination with Toxocara canis and Toxocara cati eggs in rural and urban areas of Wielkopolska District in 2000-2005
Autorzy:
Mizgajska-Wiktor, H.
Jarosz, W.
Powiązania:
https://bibliotekanauki.pl/articles/2143943.pdf
Data publikacji:
2007
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Wielkopolska
srodowisko miejskie
Toxocara canis
skazenia gleby
Toxocara cati
srodowisko wiejskie
parazytologia
pasozyty
jaja
skazenia biologiczne
Opis:
The aim of the studies was to compare the degree of soil contamination with Toxocara canis and T. cati eggs in rural and urban areas depending on time of sampling and type of places examined. Material and methods. Over 2000−2005 a total of 538 soil samples from 3 villages and 368 from Poznań city (Poland) areas were examined for Toxocara spp. eggs. In spring 418 samples in rural areas and 184 samples in urban areas were collected and in autumn 120 and 184 respectively. The samples were examined using flotation technique in saturated sodium nitrate. The discrimination of T. canis and T. cati eggs was based on the size of eggs and transparency of shell layers. Results. The contamination of soil with Toxocara eggs was higher in the urban areas (19.8% positive samples) than in the rural ones (15.6% positive samples) and city or village−backyards were most heavily contaminated. Both, in the villages and in the city, the degree of soil contamination with eggs in spring and autumn was similar (17.6 and 14.8% positive samples respectively). T. cati eggs were much more prevalent in urban areas (97% of all eggs recovered) while T. canis in rural areas (84% of all recovered eggs). The share of T. canis and T. cati eggs in soil contamination did not depend on the time of sampling.
Źródło:
Wiadomości Parazytologiczne; 2007, 53, 3; 219-225
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Porownanie skazenia gleby jajami Toxocara canis i Toxocara cati w srodowisku wiejskim i miejskim w Wielkopolsce w latach 2000-2005
A comparison of soil contamination with Toxocara canis and Toxocara cati eggs in rural and urban areas of Wielkopolska District in 2000-2005
Autorzy:
Mizgajska-Wiktor, H.
Jarosz, W.
Powiązania:
https://bibliotekanauki.pl/articles/836555.pdf
Data publikacji:
2007
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Wielkopolska
srodowisko miejskie
Toxocara canis
skazenia gleby
Toxocara cati
srodowisko wiejskie
parazytologia
pasozyty
jaja
skazenia biologiczne
Źródło:
Annals of Parasitology; 2007, 53, 3; 219-225
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
IIIrd National Scientific Conference on Trichinella and trichinellosis in Poznan
Autorzy:
Kociecka, W.
Powiązania:
https://bibliotekanauki.pl/articles/839040.pdf
Data publikacji:
1997
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
epizootic problem
man
Trichinella
Polska
trichinellosis
Trichinella britovi
Poznan city
Wielkopolska region
wild fauna
clinical pathology
epidemiological problem
Źródło:
Annals of Parasitology; 1997, 43, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Występowanie Entamoeba histolytica i innych pierwotniaków jelitowych wśród ludności Wielkopolski w okresie ostatnich 30 lat
Prevalence of Entamoeba histolytica and other intestinal protozoa among the inhabitants of Poznan province in the period of 30 years
Autorzy:
Kasprzak, W.
Mazur, T.
Karlewiczowa, R.
Powiązania:
https://bibliotekanauki.pl/articles/2152477.pdf
Data publikacji:
1989
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
parazytologia lekarska
pasozyty czlowieka
pasozyty jelitowe
pierwotniaki
Entamoeba histolytica
Entamoeba hartmanni
Entamoeba nana
Entamoeba coli
Giardia intestinalis
Dientamoeba fragilis
wystepowanie
czestosc wystepowania
ludnosc
Wielkopolska
lata 1958-1986
Opis:
The aim of the study was to present the past and actual prevalence of Entamoeba histolytica and other intestinal protozoa in Poznań Province. Although in Poland there are only few accurate data concerning the prevalence of intestinal protozoa, the results of some studies were found reliable. In a survey conducted in 1958 in an orphanage in Poznań (Kasprzak and Karlewiczowa) the intestinal protozoa were found with high prevalence (Table 1). After six years this prevalence decreased considerably (Karlewiczowa and Kasprzak, 1964). Because at the same time such a decrease in intestinal protozoa prevalence, especially that in Entamoeba coli, was observed in the whole population of the Poznań Province, long-term surveys were undertaken. The diagnosing of intestinal protozoa in faeces of 4165 persons, performed at more points in time in the period of 1963 to 1986 in the Poznań Province, were conducted by the same highly skilled staff. Decreasing rates of infection were seen for each amoeba species at nearly each point in time, particularly in the first eight years of observation (Table 2). By using the "amoebic index" and "amoebic prevalence rate" in assessing epidemiological factors (acc. WHO, 1969), the considerable reduction in both indices should be accounted for the improvement of the sanitation and socio-economic status in our country. After the Second World War some Polish authors expressed the fear that after returning to the country, the Polish soldiers who had fought in the endemic regions of invasive amoebiasis, infected with E. histolytica, would constitute sources for spreading amoebiasis and that the disease would be a nucleus of a new, hitherto unknown, social calamity in Poland. Fortunately, the fear did not come true. Although in this country the infection with amoebae, also with E. histolytica, decreased considerably after several years, unfortunately, some opinions still prevail, being the source of an amoebo-mania in the medical staff and corresponding amoebophobia in the patients.
Źródło:
Wiadomości Parazytologiczne; 1989, 35, 6; 535-545
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-13 z 13

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