- Tytuł:
- Potential applications of SOS-GFP biosensor to in vitro rapid screening of cytotoxic and genotoxic effect of anticancer and antidiabetic pharmacist residues in surface water
- Autorzy:
-
Matejczyk, M.
Rosochacki, S. J. - Powiązania:
- https://bibliotekanauki.pl/articles/124844.pdf
- Data publikacji:
- 2015
- Wydawca:
- Polskie Towarzystwo Inżynierii Ekologicznej
- Tematy:
-
SOS-gfp biosensor
cytotoxicity
genotoxicity
cyclophosphamide
metformin - Opis:
- Escherichia coli K-12 GFP-based bacterial biosensors allowed the detection of cytotoxic and genotoxic effect of anticancer drug– cyclophosphamide and antidiabetic drug – metformin in PBS buffer and surface water. Experimental data indicated that recA::gfpmut2 genetic system was sensitive to drugs and drugs mixture applied in experiment. RecA promoter was a good bioindicator in cytotoxic and genotoxic effect screening of cyclophosphamide, metformin and the mixture of the both drugs in PBS buffer and surface water. The results indicated that E. coli K-12 recA::gfp mut2 strain could be potentially useful for first-step screening of cytotoxic and genotoxic effect of anticancer and antidiabetic pharmacist residues in water. Next steps in research will include more experimental analysis to validate recA::gfpmut2 genetic system in E. coli K-12 on different anticancer drugs.
- Źródło:
-
Journal of Ecological Engineering; 2015, 16, 1; 116-121
2299-8993 - Pojawia się w:
- Journal of Ecological Engineering
- Dostawca treści:
- Biblioteka Nauki
Artykuł