- Tytuł:
- Evaluation of MR relaxation times following trastuzumab treatment of breast cancer cells in a 3D bioreactor
- Autorzy:
-
Bartusik-Aebisher, Dorota
Aebisher, David
Czmil, Anna
Mazur, Damian - Powiązania:
- https://bibliotekanauki.pl/articles/895234.pdf
- Data publikacji:
- 2020-02-29
- Wydawca:
- Polskie Towarzystwo Farmaceutyczne
- Tematy:
-
magnetic resonance imaging (MRI)
breast cancer cell cultures
hollow-fiber bioreactor (HFBR)
relaxation time maps - Opis:
- The three dimensional tumor environment can be mimicked with the use of cells cultured within a hollow-fiber bioreactor (HFBR). In this work, magnetic resonance imaging (MRI) was used to monitor changes in relaxation time in breast cancer cells following treatment with Trastuzumab in a HFBR system. Breast cancer cells were inoculated into the HFBR system and cultured over a period of 4 weeks. Relaxation time maps were generated according to MRI techniques in three dimensional (3D) cultures of MCF7/Her2 breast cancer and MCF7/Neo4 control cells. MRI measurements showed a variation in values of spin-lattice (T1) and spin-spin (T2) relaxation times related to cell density. Differences in both values (T1 and T2) were noted between untreated cells and cells treated with trastuzumab in the HFBR device. Additionally, 1H MRI was able to provide information about drug penetration in breast cancer cell culture organized in 3D. In conclusion, MRI in vitro can provide direct, noninvasive visualization of cell density in 3D geometry and cell viability as a function of drug uptake. Both T1 and T2 values were higher for lower cell density and accordingly both values, T1 and T2, were lower for higher cell density.
- Źródło:
-
Acta Poloniae Pharmaceutica - Drug Research; 2020, 77, 1; 35-41
0001-6837
2353-5288 - Pojawia się w:
- Acta Poloniae Pharmaceutica - Drug Research
- Dostawca treści:
- Biblioteka Nauki
Artykuł