- Tytuł:
- Development of the UHPLC-DAD method for simultaneous determination of four active pharmacological ingredients in cannabinoid plant material
- Autorzy:
-
Zalewski, Przemysław
Krause, Anna
Milewski, Marek
Wielgus, Karolina
Szalata, Milena
Słomski, Ryszard
Piontek, Judyta C. - Powiązania:
- https://bibliotekanauki.pl/articles/895446.pdf
- Data publikacji:
- 2018-08-31
- Wydawca:
- Polskie Towarzystwo Farmaceutyczne
- Tematy:
-
validation
UHPLC-DAD
cannabinoid extract - Opis:
- A stability-indicating ultra-high-performance liquid chromatography (UHPLC) with DAD detector was developed and validated for the simultaneously determination of Δ9-tetahydrocannabitol (THC), cannabidiol (CBD), Δ9-tetahydrocannabinolic acid (THC-COOH) and cannabidiolic acid (CBD-COOH) in extracts of Cannabis sativa L.. The separation was achieved on a Kinetex, C18, 100 x 2.1 mm, 2.6 µm. The gradient separation was started with mixture of methanol and 12mM ammonium acetate in ratio 75:25 (%/%). The methanol was increased to 100% from 0.0 min to 10.0 min, then maintained 100% for the next 1.0 min, then decreased to 75% from 11.0 min to 15.0 min and held at 75% from 15.0 min to 20.0 min. The injection volume was 5.0 µL and the wavelength of detection was controlled at 228 nm.
- Źródło:
-
Acta Poloniae Pharmaceutica - Drug Research; 2018, 75, 4; 885-890
0001-6837
2353-5288 - Pojawia się w:
- Acta Poloniae Pharmaceutica - Drug Research
- Dostawca treści:
- Biblioteka Nauki
Artykuł