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    Wyświetlanie 1-3 z 3
    Tytuł:
    Chemia bioortogonalna – użyteczne narzędzie badania procesów wewnątrzkomórkowych
    Bioortogonal chemistry - a useful tool for studying intercellular processes
    Autorzy:
    Latos, Krystian
    Powiązania:
    https://bibliotekanauki.pl/articles/2200602.pdf
    Data publikacji:
    2022
    Wydawca:
    Polskie Towarzystwo Chemiczne
    Tematy:
    chemia bioortogonalna
    bioortogonalna ligacja
    kwasy nukleinowe
    białka
    sacharydy
    bioorthogonal chemistry
    bioorthogonal ligation
    nucleic acids
    proteins
    saccharides
    Opis:
    Bioorthogonal chemistry is a rapidly developing field of science operating on the border of chemistry and biology. Its initial goal was to study metabolism and imaging using fluorescently labelled compounds. Due to recent advances, bioorthogonal chemistry can also be used to engineer therapeutic bioconjugates. By using a combination of bioconjugation and advanced omics techniques, it is possible to study and modify complex interactions inside living cells. In the relatively short time since its introduction, bioorthogonal chemistry has found many applications. In nucleic acid research, it is used for labelling, e.g. with biotin, to facilitate detection, immobilization, and purification. Additionally, thanks to the use of fluorescent nucleoside analogues, it can be used to study the interaction and dynamics of nucleic acids. For the study of proteins, bioorthogonal chemistry is an invaluable tool for studying conformation, as well as intramolecular and intermolecular interactions. Using techniques such as PET and FRET it is possible to take a closer look at the structure of proteins, which has a significant impact on their functionality. By using biarsenical dyes, interactions between proteins are tracked. This is used in the study of protein aggregation in diseases such as Alzheimer's, Huntington's, and prion diseases. Thanks to this, it becomes possible to understand the mechanism and pathology of these diseases. In biosensing, the elements of bioorthogonal chemistry have been used in a variety of tests and imaging methods. In the end, methods for testing glycan are presented. The advantage of bioorthogonal methods is that they allow labelling on the whole cell or lysate. This application in glycoproteomics is extremely important due to the fact that changes in glycosylation occur during disease states.
    Źródło:
    Wiadomości Chemiczne; 2022, 76, 1-2; 79-95
    0043-5104
    2300-0295
    Pojawia się w:
    Wiadomości Chemiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Oligonukleotydy DNA jako warstwy receptorowe sensorów elektrochemicznych
    DNA oligonucleotides as receptor layers of electrochemical sensors
    Autorzy:
    Górski, Ł.
    Ziółkowski, R.
    Bala, A.
    Jarczewska, M.
    Malinowska, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/171556.pdf
    Data publikacji:
    2015
    Wydawca:
    Polskie Towarzystwo Chemiczne
    Tematy:
    biosensor
    metale ciężkie
    kwasy nukleinowe
    samoorganizujące się monowarstwy
    woltamperometria
    znaczniki redoks
    biosensors
    heavy metals
    nucleic acids
    self-assembled monolayers
    voltammetry
    redox indicators
    Opis:
    The need for elaboration of analytical devices of small dimensions and the accessibility of novel nanomaterials caused the increase in the number of publications referring to the development of biosensors. DNA-based biosensors are of special interest and they were primarily used for the determination of a specific sequence which is crucial in the detection of cancer, genetic mutations, pathogens, as well as analysis of modified food. Interestingly, they could be also applied for the detection of other analytes including heavy metal ions, especially in connection with electrochemical techniques. It should be noted that the design of DNA biosensor concerns not only the development of transducer, but also careful preparation of sensing layer and the choice of the method of analytical signal generation. Selectivity is one of the essential parameter of the biosensor that determines its utility, particularly in real samples of complex matrices. In case of DNA sensors dedicated for the detection of complementary sequence, high selectivity is provided by the hybridization process. A pronounced specificity of sensing layer-analyte interaction can be also achieved with the use of functional nucleic acids - aptamers, which change their conformation upon binding an analyte. Herein, DNA-modified electrodes were firstly used for the detection of uranyl ions, as they exhibit high affinity towards phosphate moieties of nuclec acids. It was shown that UO2 2+ interacts with sensing layer independently from the chosen oligonucleotide sequence. Moreover, the influence of Pb2+ was reduced by elimination of adenine, which strongly interacts with lead ions. Another oligonucleotide-based sensor was developed for detection of mercury ions. The results indicate that Hg2+ concentration can be determined only with the use of sequence containing 100% thymine residues. Oligonucleotide-based sensor with receptor layer containing aptamers was elaborated for the detection of Pb2+ ions. In the presence of lead cations, an aptamer probe forms a G-quadruplex structure, a proposed biosensor could be characterized with selectivity towards Pb2+ The performance of DNA-based sensors for UO2 2+, Hg2+ and Pb2+ ions was optimized and addressed the choice of the manner of analytical signal generation, the influence of electrode modification with blocking agent, sensitivity dependence on the oligonucleotide sequence and the possibility of regeneration of sensing layer. Finally, the utility of proposed DNA sensors was tested by analysis in real samples.
    Źródło:
    Wiadomości Chemiczne; 2015, 69, 5-6; 325-336
    0043-5104
    2300-0295
    Pojawia się w:
    Wiadomości Chemiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Modyfikowane oligodeoksyrybonukleotydy zawierające w wiązaniu internukleotydowym w pozycji mostkowej atom azotu
    Modified oligodeoxyribonucleotides containing nitrogen at a bridging position of an internucleotide bond
    Autorzy:
    Radzikowska, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/172442.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Towarzystwo Chemiczne
    Tematy:
    analogi kwasów nukleinowych
    modyfikacje wiązania internukleotydowego
    oligodeoksyrybonukleozydo-(P3’→N5’)amidofosforany
    oligodeoksyrybonukleozydo-(N3’→P5’)amido(tio)fosforany
    strategia antysensowa
    telomeraza
    reakcja Athertona-Todda
    nucleic acids analogues internucleotide linkage modifications
    oligodeoxyribonucleoside-(P3’→N5’)phosphoramidates
    oligodeoxyribonucleoside-
    (N3’→P5’)(thio)phosphoramidates antisense strategy
    telomerase
    Atherton-Todd reaction
    Opis:
    Synthetic oligonucleotides (ONs) constitute an important class of compounds which exhibit biological activity. As potential drugs ONs are employed in the antisense strategy [1]. The antisense therapeutic agent acts on the pathogenic mRNA causing inactivation of the target. Ideal antisense agent should be resistant to exo and/or endonucleases, have a suitable pharmacological and pharmacokinetic profile and high affinity for the target. To improve some properties of antisense oligonucleotides plethora of chemical modifications introduced within both sugar unit and internucleotides linkage were investigated. Among numerous ONs modified in internucleotide phosphodiester bond, one of the most interesting are oligonucleotide phosphoramidates (NP-oligos) in which one of the bridging oxygens is replaced by nitrogen atom (at 3’ or 5’ position). Hence, two classes of compounds are formed: oligonucleotide-(N5’→P3’)phosphoramidates and oligonucleotide(N3’→P5’)-phosphoramidates. These compounds, similar to native DNA and RNA, possess an achiral phosphorous atom and all internucleotides bonds are negatively charged. Additionally, NP-oligo shows good resistance to nucleolytic degradation and can bind to the target DNA or RNA with high affinity [12]. In literature several synthetic strategies concerning both (N5’→P3’) and (N3’→P5’) NP-oligos have been described. Some of them allowed to obtain only corresponding dimers. In the light of recent discoveries the most promising candidates for therapeutic and diagnostic applications are oligonucleotide-(N3’→P5’)thiophosphoramidates. Gryaznov et al. have found that such compounds can act as potent and selective telomerase inhibitors [29]. Human telomerase (TA) is a reverse transcriptase ribonucleoprotein that synthesizes de novo d-(TTAGGG)n repeats at chromosomal DNA ends. Whereas activity of this enzyme is observed in ~85% of all human tumors, most of normal somatic cells either lack TA activity or express it only at low levels. For these reasons TA constitute an attractive and nearly universal anticancer target for rational drug development.
    Źródło:
    Wiadomości Chemiczne; 2013, 67, 11-12; 1003-1025
    0043-5104
    2300-0295
    Pojawia się w:
    Wiadomości Chemiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-3 z 3

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