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    Wyświetlanie 1-5 z 5
    Tytuł:
    Nuclear morphology, polyploidy, and chromatin elimination in tissue culture of Allium fistulosum L.
    Autorzy:
    Joachimiak, A
    Ilnicki, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/59216.pdf
    Data publikacji:
    2003
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    chromatin
    heterochromatin
    tissue culture
    polyploidy
    Alliaceae
    morphology
    Monocotyledoneae
    micronucleus
    Allium fistulosum
    Opis:
    The morphology of cell nuclei in callus obtained from root-tip meristems of Allium fistulosum L. (Monocotyledoneae, Alliaceae) was analysed. The most interesting phenomena observed in long-term callus culture were the different mechanisms of cell polyploidization, enlargement of telomeric segments of heterochromatin, and extensive chromatin elimination, associated with instability of nuclei size and DNA content. Protruding heterochromatin "spikes" were observed on the surface of some di- and polyploid nuclei. The presence of these spikes was connected with the formation of small heterochromatic micronuclei frequently found in the cytoplasm. It is suggested that these micronuclei are produced by direct elimination of heterochromatin from the interphase nuclei. Polyploid cells accumulated with each successive cell collection. The ploidy level attained by highly polyploid cells was 15C-220C. The shape of the nuclei and heterochromatin distribution suggest that polyploid nuclei in A. fistulosum tissue culture are produced by endoreduplication and by restitution cycles.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2003, 72, 1
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Protocol for acclimatization of in vitro cultured Potamogeton praelongus - aspect of plantlet size and type of substrate
    Autorzy:
    Prausova, R.
    Kozelkova, Z.
    Safarova, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/57604.pdf
    Data publikacji:
    2015
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    acclimatization
    in vitro culture
    Potamogeton praelongus
    plant tissue culture
    growth experiment
    plantlet size
    substrate type
    Opis:
    The aim of the experiment was to determine suitable substrate type and optimal plant size for transfer of plantlets from in vitro to ex vitro under experimental outdoor conditions. Tests focused on the effect of substrate type (muddy and sandy) and starting size of plantlets gained through in vitro seed germination (0-3, 3.1-5,5.1-6, 6.1-10 cm) on plant growth. Three parameters (fresh weight, length, and the number of leaves) were compared to evaluate growth. Basic water parameters in experimental water tanks were regularly measured (pH, temperature, electrical conductivity, shadow intensity) and controlled to reach similar conditions to those in the natural habitat of this species. Overwintering was studied in a cellar with newly defined size categories (<6, 6.1-8, 8.1-10, 10.1-12, 12.1-15 cm). Both substrate type and starting size of plantlets significantly impacted growth. Plantlets grew better in the muddy substrate while a 100% success rate of rooting was gained with a starting size of 6.1-10 cm in both substrates. The biggest increase in fresh weight was observed with a starting size of 3.1-5 cm and 5.1-6 cm in both substrates. The greatest increase in fresh weight was observed in plants with a starting size of 3.1-5 cm in the muddy substrate (more than 95% increase). The best overwintering results were gained in the 6.1-8 cm size category.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2015, 84, 1
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Propagation of goldenrod (Solidago canadensis L.) from leaf and nodal explants
    Autorzy:
    Li, J.
    Kang, Y.
    Qiang, S.
    Peng, G.
    Powiązania:
    https://bibliotekanauki.pl/articles/56457.pdf
    Data publikacji:
    2012
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    propagation
    goldenrod
    Solidago canadensis
    leaf
    nodal explant
    callus formation
    micropropagation
    adventitious shoot
    tissue culture
    Opis:
    Goldenrod (Solidago canadensis L.) is an invasive plant species in many countries except North America but a cut-flower species worldwide. There is a need to generate and propagate goldenrod clones efficiently for research and commercial purposes. A callus induction and plantlet regeneration system was developed by studying the influence of explant type and different concentrations of plant growth regulators. The highest callus production from leaf segments was obtained on Murashige and Skoog's medium (MS medium) supplemented with 1.0 mg/L naphthalene acetic acid (NAA) and 1.0 mg/L 6-benzylaminopurine (BA). Adventitious shoots could be regenerated directly from leaf explants without an intermediate callus phase with the highest shoot induction percentage of 87.2%. The largest number of adventitious shoots per leaf explant (3.2) was obtained on MS medium supplemented with 0.4 mg/L NAA and 2.0 mg/L BA. MS medium supplemented with 0.1 mg/L NAA and 1.0 mg/L BA was the best medium for axillary shoot regeneration from nodal segments. The highest root number and longest roots occurred on half-strength MS without the addition of any growth regulator. Rooted plantlets were then transferred to a soil-based growth medium, placed in a greenhouse, and acclimatized with 100% success. All surviving plants grew normally without showing any morphological variation when compared to those grow from seed. This regeneration protocol may be used to produce certain biotypes of goldenrod suitable for genetic transformation, rapid propagation of goldenrod for commercial purposes or for screening fungi and toxins as potential biocontrol agents against this weed.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2012, 81, 1
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The influence of PPM upon the sterility of the in vitro cultures in European beech [Fagus sylvatica L.]
    Autorzy:
    Kraj, W
    Dolnicki, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/56550.pdf
    Data publikacji:
    2003
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    Plant Preservative Mixture
    plant physiology
    tissue culture
    tree bud
    Fagus sylvatica
    sterilization
    beech
    sterility
    in vitro
    embryo
    European beech
    bud
    Opis:
    Investigations were conducted on the strongly infested by microbes embryos isolated from seed stratified in non-sterile conditions or from tree buds. The results showed, that PPM (Plant Preservative Mixture, produced by Plant Cell Technology, Inc.) was very useful for increasing the percentage of sterile cultures disinfected in a 5% solution of NaOCl. The best way of applying PPM turned out to be adding the substance directly to the growing medium. At the concentration of 2 or 4 cm3 x dm-3 of PPM there was an 30% increase in percentage of sterile of in vitro cultures developed from embryos and 70-80% of cultures developed from buds, as compared with the control without PPM. At the range of concentrations employed in this study, no negative effects of PPM upon the development and growth of callus were observed.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2003, 72, 4
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    65 years of in vitro culture in Poland
    Autorzy:
    Zenkteler, M.
    Zenkteler, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/58090.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    in vitro culture
    history
    plant tissue
    in vitro technology
    Polska
    Opis:
    This paper is a short review of historical development of the tissue culture method in Poland. Similarly to the rest of the world, in vitro technology in Poland has progressed in many directions simultaneously. Its main fields are closely interconnected by natural sequences of biological processes and integrade one into another. The best results, driven by the prospects of practical applications, have been achieved within the areas of meristem culture and micropropagation of valuable genotypes, somaclonal variation, production of haploids and DH plants, somatic embryogenesis, in vitro culture of embryos and ovules, improvement of plant disease resistance, somatic hybridization and transformation of plants. Due to the fact that in vitro domain is a very broad science the authors are aware that this review might not fully cover all the scientists working with tissue culture and their achievements.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2013, 82, 3
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-5 z 5

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