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Wyświetlanie 1-2 z 2
Tytuł:
Adventitious bud regeneration from the stigma of Sinapis alba L.
Regeneracja pąków przybyszowych ze znamion Sinapis alba L.
Autorzy:
Zarychta, L.
Zenkteler, E.
Powiązania:
https://bibliotekanauki.pl/articles/28578.pdf
Data publikacji:
2010
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
adventitious bud
bud
regeneration
Sinapis alba
stigma
white mustard
plantlet development
Brassicaceae
Opis:
Stigmas isolated from flower buds of ‘Nakielska’ variety of Sinapis alba were used to develop a micropropagation method suitable for breeding of new cultivars. The origin of adventitious bud regeneration was studied on MS medium, under stimulation by bezylaminopurine (BAP) in combination with 2,4-D - naphtoxy acetic acid (2,4-D). Histological analysis showed the structure of Sinapis stigma (composed from four types of tissue: papillae, transmitting tissue, parenchyma and vascular bundles) and revealed that numerous meristematic centers developed from parenchyma cells in close vicinity of vascular bundles. Buds very quickly appeared on the surface of initial explants and later formed multiplantlets that were easily rooted in the soil.
Źródło:
Acta Agrobotanica; 2010, 63, 1
0065-0951
2300-357X
Pojawia się w:
Acta Agrobotanica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Protocol for acclimatization of in vitro cultured Potamogeton praelongus - aspect of plantlet size and type of substrate
Autorzy:
Prausova, R.
Kozelkova, Z.
Safarova, L.
Powiązania:
https://bibliotekanauki.pl/articles/57604.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
acclimatization
in vitro culture
Potamogeton praelongus
plant tissue culture
growth experiment
plantlet size
substrate type
Opis:
The aim of the experiment was to determine suitable substrate type and optimal plant size for transfer of plantlets from in vitro to ex vitro under experimental outdoor conditions. Tests focused on the effect of substrate type (muddy and sandy) and starting size of plantlets gained through in vitro seed germination (0-3, 3.1-5,5.1-6, 6.1-10 cm) on plant growth. Three parameters (fresh weight, length, and the number of leaves) were compared to evaluate growth. Basic water parameters in experimental water tanks were regularly measured (pH, temperature, electrical conductivity, shadow intensity) and controlled to reach similar conditions to those in the natural habitat of this species. Overwintering was studied in a cellar with newly defined size categories (<6, 6.1-8, 8.1-10, 10.1-12, 12.1-15 cm). Both substrate type and starting size of plantlets significantly impacted growth. Plantlets grew better in the muddy substrate while a 100% success rate of rooting was gained with a starting size of 6.1-10 cm in both substrates. The biggest increase in fresh weight was observed with a starting size of 3.1-5 cm and 5.1-6 cm in both substrates. The greatest increase in fresh weight was observed in plants with a starting size of 3.1-5 cm in the muddy substrate (more than 95% increase). The best overwintering results were gained in the 6.1-8 cm size category.
Źródło:
Acta Societatis Botanicorum Poloniae; 2015, 84, 1
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-2 z 2

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