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    Wyświetlanie 1-4 z 4
    Tytuł:
    Effects of selenium on the growth and photosynthetic characteristics of flue-cured tobacco (Nicotiana tabacum L.)
    Autorzy:
    Jiang, C.
    Zu, C.
    Shen, J.
    Shao, F.
    Li, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/57536.pdf
    Data publikacji:
    2015
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    selenium
    growth
    photosynthesis
    tobacco plant
    Nicotiana tabacum
    carboxylation efficiency
    Opis:
    The objective of this study was to investigate the effect of Selenium (Se) supply (0, 3, 6, 12, 24 mg kg−1) on the growth, photosynthetic characteristics, Se accumulation and distribution of flue-cured tobacco (Nicotiana tabacum L.). Results showed that low-dose Se treatments (≤6 mg kg−1) stimulated plant growth but high-dose Se treatments (≥12 mg kg−1) hindered plant growth. Optimal Se dose (6 mg kg−1) stimulated plant growth by reducing MDA content and improving photosynthetic capability. However, excess Se (24 mg kg−1) increased MDA content by 28%, decreased net photosynthetic rate and carboxylation efficiency by 34% and 39%, respectively. The Se concentration in the roots, stems, and leaves of the tobacco plants significantly increased with increasing Se application. A linear correlation (R = 0.95, P < 0.01) was observed between Se level and tobacco plant tissue Se concentration. This correlation indicated that the tobacco plant tissues were not saturated within the concentration range tested. The pattern of total Se concentration in the tobacco plant tissues followed the order root > leaf > stem. The Se concentration in the roots was 3.17 and 7.57 times higher than that in the leaves and stems, respectively, after treatment with 24 mg kg−1 Se. In conclusion, the present study suggested that optimal Se dose (6 mg kg−1) improved the plant growth mainly by enhancing photosynthesis, stomatal conductance, carboxylation efficiency and Rubisco content in the flue-cured tobacco leaves. However, the inhibition of excess Se on tobacco growth might be due to high accumulation of Se in roots and the damage of photosynthesis in leaves.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2015, 84, 1
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Changes accompanying proliferative capacity and morphology of Nicotiana tabacum L. callus in response to 2,4-D
    Zmiany związane ze zdolnością do proliferacji i morfologią kalusa Nicotiana tabacum L. w odpowiedzi na różne stężenia 2,4-D
    Autorzy:
    Gatz, Andrzej
    Tomaszewska-Sowa, Agnieszka
    Figas, Anna
    Powiązania:
    https://bibliotekanauki.pl/articles/1628038.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    Nicotiana tabacum
    2
    4-dichlorophenoxyacetic acid
    proliferation
    cell divisions
    callus morphology
    Opis:
    The common trait of all auxins is a stimulation of cell elongation and also cell division in the presence of cytokinin; both are essential for callus induction and its multiplication. The response of plant tissues to various compounds with auxin activity may be quite different. In this study, the effectiveness of a synthetic auxin, 2,4-dichlorofenoxyacetic acid (2,4-D), instead of the generally applied natural auxin, indole-3-acetic acid (IAA), was tested for the proliferation of Nicotiana tabacum callus. The following concentrations of 2,4-D were tested: 0.1, 0.5, 1.0, 1.5, and 2.0 mg dm−3. Callus was derived from stem pith and its proliferation allowed on MS medium through five subcultures at 25°C and in darkness. After each passage, the fresh weight and morphological features of the callus were determined. The 0.5 mg dm−3 2,4-D treatment was the most favorable for producing the greatest increase in fresh weight in each of five subsequent subcultures as well as maintaining normal morphological features for proliferation. However, the 1.0 mg dm−3 2,4-D treatment in comparison with the lowest, 0.1 mg dm−3, was more beneficial when considering regular increases of fresh weight and a better cell cohesion for callus growth.
    Do indukcji i namnażania kalusa niezbędne jest zastosowanie odpowiedniej auksyny, podanej samodzielnie lub z cytokininą. Spośród naturalnych auksyn IAA jest często wykorzystywana do stymulowania proliferacji kalusa Nicotiana tabacum. Zastosowanie IAA wymaga wyższych stężeń ze względu na nietrwałość, wynikającą z wrażliwości na światło i wysoką temperaturę. Takich ograniczeń nie posiada syntetyczna auksyna 2,4-D. Celem tej pracy była ocena przydatności 2,4-D do proliferacji kalusa Nicotiana tabacum z następującymi wariantami stężeń 0.1, 0.5, 1.0, 1.5 i 2 mg dm−3. Proliferację kalusa z rdzenia łodygi przeprowadzono na pożywce MS przez 5 pasaży bez dostępu światła i w temperaturze 25°C. Po każdym pasażu określono świeżą masę i cechy morfologiczne kalusa takie jak barwa, struktura powierzchni i stopień spójności komórek. Spośród badanych stężeń 2,4-D, najefektywniej stymulującym było 0.5 mg dm−3 ze względu na najwyższy przyrost świeżej masy w każdym z pięciu kolejnych pasaży oraz sprzyjające proliferacji cechy morfologiczne kalusa. Wariant stężenia 1.0 mg dm−3 2,4-D w porównaniu z 0.1 mg dm−3 okazał się korzystniejszy, ponieważ charakteryzował się regularnymi przyrostami i lepszą dla namnażania spójnością komórek kalusa.
    Źródło:
    Acta Agrobotanica; 2017, 70, 4
    0065-0951
    2300-357X
    Pojawia się w:
    Acta Agrobotanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Tapetum development in transgenic tobacco [Nicotiana tabacum L.] plants with modified level of histone H1 variants
    Autorzy:
    Slusarczyk, J
    Tykarska, T.
    Wierzbicki, A.
    Jerzmanowski, A.
    Kuras, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/58139.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    tapetum
    development
    transgenic plant
    tobacco
    Nicotiana tabacum
    histone level
    H1 variant
    sterile pollen grain
    Opis:
    The phenomenon of male sterility has often been observed in investigations on the role of histone H1 in regulation of morphogenetic and cytological processes in transgenic tobacco plants. These changes were accumulated by disturbances in flower development, consisting in lengthening of the pistil style in relation to stamen heads. This prevented pollination and production of seeds. As similar abnormalities occurred also in the present investigations (depending on combination, the sterility% was 84.4 to 19.9, at only 8.1 in the control), the main problem of our investigations was an attempt to explain their reasons. It is commonly known that one of the conditions for formation of fertile pollen is the properly functioning tapetum. Here, we carried out observations of ultrastructure of anther tapetum control cells in respect of abnormalities which occurred during microsporogenesis of transgenic plants with inactivated expression of two major (A, B) and two minor (C, D) histone H1 variants. The investigations were carried out on the following groups of plants: (1) control group with a full set of histone variants (K), (2) with inactivated A and B variants (-AB); (3) with inactivated A, B, C and D variants (-ABCD), (4) with inactivated C and D variants (-CD). It was found that tapetal development was normal in all the investigated groups of plants, and the sequence of changes was similar as in the control. However, certain ultrastructural differences appeared when tapetum functioned as secretory tissue, and in the degeneration phase. In tapetal cell cytoplasm, with participation of rER, lipid bodies were formed, which, having penetrated to the cell surface and to locules, took part in formation of pollen grain sporoderm. Both in the control and in the remaining combination, excluding -ABCD, these bodies looked similar: they were grey, homogenous and surrounded by black jagged deposits. In -ABCD plants, these bodies were more translucent, slightly rarefied, and not surrounded by the deposits. Moreover, in -CD plants, large lipid deposits were frequently observed between remainders of degraded tapetal cells. They did not occur in the control and the remaining combinations.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2006, 75, 2; 95-105
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Influence of change in the proportion of H1 histone variants on microsporogenesis and development of male gametophyte in transgenic plants of tobacco [Nicotiana tabacum L.]
    Autorzy:
    Slusarczyk, J
    Wierzbicki, A.
    Przewloka, M.
    Tykarska, T.
    Jerzmanowski, A.
    Kuras, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/57064.pdf
    Data publikacji:
    2003
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    sterile pollen grain
    male
    development
    transgenic plant
    gametophyte
    tobacco
    microsporogenesis disorder
    histone H1
    Nicotiana tabacum
    Opis:
    As continuation of investigations in to the mechanism of the role of the H1 histone, which is a crucial protein component chromosomes of all eukaryotes, transgenic tobacco plants with different levels of the H1 histone variants were examined. Tobacco has six sequential variants of the H1 histone: two major ones (H1A and H1B), constituting ca. 90% of all H1, and four minor ones (H1C, H1D, H1E and H1F), occurring in very small quantities. The following groups of plants were examined: K - control group with a full set of histone variants; -AB -with the A and B variants removed; -ABCD - with the A, B, C and D variants removed; and -CD - with the C and D variants removed. The analysis of microsporogenesis in those plants, based on preparations squeezed in acetoorcein, revealed the asynchronous course of meiosis in -AB and -ABCD plants, occurrence of chromosomal aberration, and, consequently, the formation of sterile pollen grains (accordingly: 84,4% and 81,4%). In -CD plants, the percentage of aberration and sterile pollen grains was similar to the control material. Electron microscope observations of microsporogenesis showed ultrastructural changes. In -AB and -ABCD plants, a major portion of the pollen grains were degraded. The smallest number of degraded pollen grains, in comparison with the control, was found in the -CD group.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2003, 72, 1
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-4 z 4

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