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Wyszukujesz frazę "Zhang, K." wg kryterium: Autor

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    Wyświetlanie 1-5 z 5
    Tytuł:
    Methyl jasmonate-induced accumulation of metabolites and transcriptional responses involved in triterpene biosynthesis in Siraitia grosvenorii fruit at different growing stages
    Autorzy:
    Zhang, K.
    Luo, Z.
    Guo, Y.
    Mo, C.
    Tu, D.
    Ma, X.
    Bai, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/59180.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Opis:
    The cucurbitane-type triterpenoid glycosides, mogrosides, are the main active components of Siraitia grosvenorii fruit. Squalene and cucurbitadienol are among the intermediates of the biosynthetic pathway for the formation of cucurbitane-type triterpenoid backbones of mogrosides. It is recognized that the exogenous application of methyl jasmonate (MeJA) increases the accumulation of secondary metabolites in various plant species. Here, the effect of MeJA (50, 200, and 500 μM) on the accumulation of squalene and cucurbitadienol in the fruits of S. grosvenorii at 10, 20, and 30 days after flowering (DAF) was tested for the first time. Since mogroside II E is the main cucurbitane-type triterpenoid present at this time, its concentration was also determined. The results show that MeJA can indeed promote squalene and cucurbitadienol accumulation, the application of 500 μM MeJA at 30 DAF being optimal. The concentration of squalene and cucurbitadienol increased up to 0.43 and 4.71 μg/g dry weight (DW), respectively, both of which were 1.2-fold greater than that of the control. The content of mogroside II E increased by 15% over the untreated group. We subsequently analyzed the expression of key genes involved in the mogroside biosynthetic pathway, including the 3-hydroxy-3-methylglutaryl-coenzyme A reductase gene (SgHMGR), squalene synthetase gene (SgSQS), cucurbitadienol synthase gene (SgCS), and cytochrome P450 (SgCYP450) with quantitative real-time PCR. The results showed that transcriptional levels of these genes were upregulated following the treatment described above. Additionally, their responses in the presence of MeJA was related to the concentration and timing of MeJA treatment.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2016, 85, 3
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Identification of medicinal plant Schisandra chinensis using a potential DNA barcode ITS2
    Autorzy:
    Li, X.-K.
    Wang, B.
    Han, R.-C.
    Zheng, Y.-C.
    Yin, H.-B.
    Xu, L.
    Zhang, J.-K.
    Xu, B.-L.
    Powiązania:
    https://bibliotekanauki.pl/articles/56996.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    identification
    internal transcribed spacer
    medicinal plant
    Schisandra chinensis
    DNA barcode
    Opis:
    To test whether the internal transcribed spacer 2 (ITS2) region is an effective marker for using in authenticating of the Schisandra chinensis at the species and population levels, separately. And the results showed that the wild populations had higher percentage of individuals that had substitution of C→A at site 86-bp than the cultivated populations. At sites 10-bp, 37-bp, 42-bp and 235-bp, these bases of the Schisandra sphenanthera samples differed from that of S. chinensis. Two species showed higher levels of inter-specific divergence than intra-specific divergence within ITS2 sequences. However, 24 populations did not demonstrate much difference as inter-specific and intra-specific divergences were concerned. Both S. chinensis and S. sphenanthera showed monophyly at species level, yet the samples of different populations shown polyphyly at population level. ITS2 performed well when using BLAST1 method. ITS2 obtained 100% identification success rates at the species level for S. chinensis, with no ambiguous identification at the genus level for ITS2 alone. The ITS2 region could be used to identify S. chinensis and S. sphenanthera in the “Chinese Pharmacopoeia”. And it could also correctly distinguish 100% of species and 100% of genera from the 193 sequences of S. chinensis. Hence, the ITS2 is a powerful and efficient tool for species identification of S. chinensis.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2013, 82, 4
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    EGTA, a calcium chelator, affects cell cycle and increases DNA methylation in root tips of Triticum aestivum L.
    Autorzy:
    Zhang, C.
    Shi, W.
    Ma, K.
    Li, H.
    Zhang, F.
    Powiązania:
    https://bibliotekanauki.pl/articles/57455.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Opis:
    In this study, when germinated Triticum aestivum L. seeds were treated with 0, 2, 4 and 6 mM ethyl glycol tetraacetic acid (EGTA), root growth was suppressed and the mitotic index decreased. These inhibitory effects were positively correlated with EGTA concentration. RT-PCR analysis revealed that the expression of several gene markers related to the G1/S transition of the cell cycle were significantly downregulated. Confocal microscopy of Fluo-3/AM-stained roots showed chelation of nearly all of the Ca2+ within the root meristematic regions. Both random amplified polymorphic DNA (RAPD) and coupled restriction enzyme digestion-random amplification (CRED-RA) techniques showed significant increases in the levels of genomic DNA polymorphisms and degree of DNA methylation. The study provides information concerning the impact of Ca²+) chelator, EGTA, on the growth, expression of cell cycle transition marker genes, and changes in DNA structure and methylation in the wheat roots.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2016, 85, 3
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Forest in Karst Mountain sinkhole of southeastern China provides refugium for the preservation of bryophyte diversity
    Autorzy:
    Li, X.
    Zhang, Z.
    Wang, Z.
    Shi, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/2130582.pdf
    Data publikacji:
    2020
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    negative landform
    natural refuge
    complex microenvironment
    karst rocky
    desertification
    Opis:
    The negative landform of sinkholes provides belowground level refugia for a high diversity of forest species compared to the forests on the surface of surrounding karst plateaus in southeastern China. Bryophyte diversity in sinkhole forests is also likely to be high. In this study, bryophytes of an underground forest sinkhole (UFS), and two forests (Forest Karst Mountain 1; FKM1) and (Forest Karst Mountain 2; FKM2) on the surface of the karst plateau were compared to understand the role of the sinkhole forest in the conservation of bryophyte diversity and the relationships between bryophyte diversity, environmental factors, and soil nutrients. Significantly more bryophyte taxa were recorded from the sinkhole forest (71 taxa, 36 genera, 23 families) than those in the forest on the plateau surface, which was the closest to the sinkhole (FKM1; 29 bryophyte taxa, 16 genera, 12 families), and even fewer bryophytes were found in the forest more distant to the sinkhole (FKM2; 22 taxa, 17 genera, eight families). Twenty-four liverwort taxa were collected from the sinkhole forest, two from the closest surface forest (FKM1) and none from the furthest surface forest (FKM2). Ninety-three percent of the bryophytes in karst mountain sinkhole were not found on surface forest. The diversity index trend was as follows: UFS > FKM1 > FKM2, and the evenness index trend was in the opposite direction as follows: UFS < FKM1 < FKM2. The beta diversity showed that the Jaccard index among the three forests was 0–0.25, reflecting a huge difference between the sinkhole forest and the two surface forests. Canonical correspondence analysis showed that light was the most important factor affecting the distribution of bryophytes in the sinkhole forest, while temperature and humidity were key factors for the distribution of bryophytes in the two surface forests. Further, soil parameters, namely, soil organic carbon, total nitrogen, total phosphorus, total potassium, alkali- hydrolyzed nitrogen, and available phosphorus changed gradually from UFS to FKM1 and to FKM2. The sinkhole forest, located in a landform with the unique negative topography enclosed by cliffs, and with a complex microenvironment, provides a natural refuge for bryophyte species in areas where forests have been negatively impacted by karst rocky desertification.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2020, 89, 4
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Influence of selenium on growth, lipid peroxidation and antioxidative enzyme activity in melon (Cucumis melo L.) seedlings under salt stress
    Autorzy:
    Hu, K.L.
    Zhang, L.
    Wang, J.T.
    You, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/58727.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    selenium
    plant growth
    lipid peroxidation
    antioxidative enzyme
    enzyme activity
    melon
    Cucumis melo
    seedling
    salt stress
    Opis:
    The objective of this study was to investigate the effect of exogenous selenium (Se) supply (0, 2, 4, 8, 16 μM) on the growth, lipid peroxidation and antioxidative enzyme activity of 100 mM NaCl-stressed melon (Cucumis melo L.) seedlings. Salt stress significantly reduced the growth attributes including stem length, stem diameter, dry weight and increased antioxidative enzyme activity [superoxide dismutase (SOD), peroxidase (POD), catalase (CAT)]. Moreover, the plant exhibited a significant increase in electrolyte leakage and malondialdehyde (MDA) content under NaCl stress. Se supplementation not only improved the growth parameters but also successfully ameliorated the adverse effect caused by salt stress in melon seedlings. However, the mitigation of NaCl-stressed seedlings was different depending on the Se concentration. At lower concentrations (2–8 μM), Se improved growth and acted as antioxidant by inhibiting lipid peroxidation and increasing in SOD and POD enzymes activity under salt stress. At higher concentrations (16 μM), Se exerted diminished beneficial effects on growth. Whereas CAT activity was enhanced. The result indicated that Se supplementation had a positive physiological effect on the growth and development of salt-stressed melon seedlings.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2013, 82, 3
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-5 z 5

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