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    Wyświetlanie 1-5 z 5
    Tytuł:
    EGTA, a calcium chelator, affects cell cycle and increases DNA methylation in root tips of Triticum aestivum L.
    Autorzy:
    Zhang, C.
    Shi, W.
    Ma, K.
    Li, H.
    Zhang, F.
    Powiązania:
    https://bibliotekanauki.pl/articles/57455.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Opis:
    In this study, when germinated Triticum aestivum L. seeds were treated with 0, 2, 4 and 6 mM ethyl glycol tetraacetic acid (EGTA), root growth was suppressed and the mitotic index decreased. These inhibitory effects were positively correlated with EGTA concentration. RT-PCR analysis revealed that the expression of several gene markers related to the G1/S transition of the cell cycle were significantly downregulated. Confocal microscopy of Fluo-3/AM-stained roots showed chelation of nearly all of the Ca2+ within the root meristematic regions. Both random amplified polymorphic DNA (RAPD) and coupled restriction enzyme digestion-random amplification (CRED-RA) techniques showed significant increases in the levels of genomic DNA polymorphisms and degree of DNA methylation. The study provides information concerning the impact of Ca²+) chelator, EGTA, on the growth, expression of cell cycle transition marker genes, and changes in DNA structure and methylation in the wheat roots.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2016, 85, 3
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Xerophiles and other fungi associated with cereal baby foods locally produced in Uganda
    Autorzy:
    Ismail, M.A.
    Taligoola, H.K.
    Nakamya, R.
    Powiązania:
    https://bibliotekanauki.pl/articles/67513.pdf
    Data publikacji:
    2012
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    fungi
    cereal product
    baby food zob.child food
    child food
    food product
    production
    Uganda
    xerophilic fungi
    mycobiota
    spoilage
    contamination
    Opis:
    Fifty samples from five baby food products mainly made of cereal flour(s) were analyzed. The moisture contents of these products were between 11.14% and 11.9%, a level below 14.0%, the recommended level for safe storage of cereal grains and their products. The mycological analysis was carried out using the dilution plate method and two isolation media (DG18 for isolation of xerophilic fungi and DRBC for fungi in general). A total of 80 species related to 37 genera in addition to some unidentified fungal and yeast species were recorded on both media from the five products. The products were contaminated abundantly by xerophilic fungi which were occurring in 88% of food samples and accounting for 18.1% of the total CFU as recorded on DG18. The highest contamination level by xerophiles was registered in Mwebaza rice porridge (a component of rice flour) and the lowest in Mukuza (a product of maize, soyabean and sorghum flours). 11 xerophilic species were recorded of which Aspergillus and Eurotium (4 species each) were the predominant giving rise to 9.1% and 8.9% of the total CFU, with A. wentii, A. candidus, E. cristatum and E. repens were the most contaminating species. Of the fungi recorded other than xerophiles, species of Aspergillus (particularly A. flavus followed by A. niger), Penicillium (P. citrinum, P. oxalicum), Fusarium (F. solani, F. tricinctum), Cladosporium (C. sphaerospermum) and yeasts were the most predominant. Contamination of such foods is a matter of health hazard as these foods are for babies. So, the use of fresh, well-dried and uncontaminated flours for production of such foods is recommended.
    Źródło:
    Acta Mycologica; 2012, 47, 1
    0001-625X
    2353-074X
    Pojawia się w:
    Acta Mycologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Grain yield performance, correlation, and luster analysis in elite bread wheat (Triticum aestivum L.) lines
    Wydajność plonu ziarna, korelacja i analiza skupień w elitarnych liniach pszenicy (Triticum aestivum L.)
    Autorzy:
    Arain, S.M.
    Sial, M.A.
    Jamali, K.D.
    Laghari, K.A.
    Powiązania:
    https://bibliotekanauki.pl/articles/27445.pdf
    Data publikacji:
    2018
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Opis:
    Wheat is a leading cereal, playing a crucial role in feeding the hungry world and improving global food security. The present study was undertaken to comparatively analyze the extent of genetic diversity for various quantitative traits among the wheat material exotic to Pakistan, received from CIMMYT (The International Maize and Wheat Improvement Center), Mexico. Nineteen advanced lines from the Semi-Arid Wheat Yield Trial (SAWYT) were studied along with a local cultivar, considered a control (NIA-Amber). Data were recorded on nine important agro-morphic traits. The compared genotypes differed significantly (p ≤ 0.05) in the studied traits, where line V6 produced the highest mean grain yield (6,049 kg ha−1) and maximum 1,000-grain weight (45.0 g). Other lines, V19, V17, and V2, also showed superiority in yield (5,723, 5,150, and 5,067 kg ha−1, respectively). Days to heading established a significant positive association with days to maturity (r = 0.7995), plant height (r = 0.3168), spike length (r = 0.2696), and spikelets per spike (r = 0.4391). The important yield associated trait, 1,000-grain weight, had a highly significant positive correlation (r = 0.6833) with grain yield. Cluster analysis for various quantitative traits showed important information about genetic diversity for the studied traits among wheat genotypes. Hence, selection of genotypes for higher grain yield based on these traits could be useful for future breeding.
    Pszenica (Triticum aestivumL.) jest jednym z najważniejszych zbóż, odgrywającym kluczową rolę w zaspokajaniu potrzeb żywnościowych ludności i poprawie globalnego bezpieczeństwa żywnościowego. Niniejsze badania podjęto w celu przeprowadzenia analizy porównawczej stopnia różnorodności genetycznej wybranych cech ilościowych u linii pszenicy egzotycznych dla Pakistanu, otrzymanymi z Meksyku (CIMMYT; The International Corn and Wheat Impro- vement Center). Dziewiętnaście linii pochodzących z Semi-Arid Wheat Yield Trial (SAWYT) badano razem z lokalną odmianą NIA-Amber, przyjętą jako kontrola. Dane rejestrowano dla dziewięciu ważnych cech agro-morfologicznych. Porównywane genotypy różniły się istotnie (p≤ 0,05) pod względem badanych cech. Linia V6 dała najwyższy średni plon ziarna (6049 kg ha−1) i największą masę 1000 ziarniaków (45,0 g). Linie V19, V17 i V2 również wykazały wysoki plon (odpowiednio 5723, 5150 i 5067 kg ha−1). Liczba dni do kłoszenia była istotnie dodatnio skorelowana z wysokością roślin (r= 0,7995), dniami do dojrzałości (r= 0,3168), długością kłosa ( r= 2696) i liczbą kłosków w kłosie (r= 0,4391). Ważna cecha wpływająca na plon – masa 1000 ziarniaków – była wysoce dodatnio skorelowana z plonem ziarna (r= 0,6833). Analiza skupień dla różnych cech ilościowych dostarczyła ważnych informacji o różnorodności genetycznej badanych cech między genotypami pszenicy, dlatego też wybór genotypów dla uzyskania wyższego ziarna w oparciu o te cechy może być przydatny do przyszłej hodowli.
    Źródło:
    Acta Agrobotanica; 2018, 71, 4
    0065-0951
    2300-357X
    Pojawia się w:
    Acta Agrobotanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genome size and phenotypic variation of Nymphaea (Nymphaeaceae) species from Eastern Europe and temperate Asia
    Autorzy:
    Dabrowska, M.A.
    Rola, K.
    Volkova, P.
    Suda, J.
    Zalewska-Galosz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/59355.pdf
    Data publikacji:
    2015
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    genome size
    phenotypic variation
    Nymphaea alba
    Nymphaea candida
    Nymphaea tetragona
    Nymphaea x borealis
    Nymphaeaceae
    morphometric analysis
    flow cytometry
    interspecies hybridization
    Eastern Europe
    temperate region
    Asia
    Opis:
    Despite long-term research, the aquatic genus Nymphaea still possesses major taxonomie challenges. High phenotypic plasticity and possible interspecific hybridization often make it impossible to identify individual specimens. The main aim of this study was to assess phenotypic variation in Nymphaea taxa sampled over a wide area of Eastern Europe and temperate Asia. Samples were identified based on species-specific genome sizes and diagnostic morphological characters for each taxon were then selected. A total of 353 specimens from 32 populations in Poland, Russia and Ukraine were studied, with nine biometric traits being examined. Although some specimens morphologically matched N. xborealis (a hybrid between N. alba and N. Candida) according to published determination keys, only one hybrid individual was revealed based on genome size data. Other specimens with intermediate morphology possessed genome size corresponding to N. alba, N. Candida or N. tetragona. This indicates that natural hybridization between N. alba and N. Candida is not as frequent as previously suggested. Our results also revealed a considerably higher variation in the studied morphological traits (especially the quantitative ones) in N. alba and N. Candida than reported in the literature. A determination key for the investigated Nymphaea species is provided, based on taxonomically-informative morphological characters identified in our study.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2015, 84, 2
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Methyl jasmonate-induced accumulation of metabolites and transcriptional responses involved in triterpene biosynthesis in Siraitia grosvenorii fruit at different growing stages
    Autorzy:
    Zhang, K.
    Luo, Z.
    Guo, Y.
    Mo, C.
    Tu, D.
    Ma, X.
    Bai, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/59180.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Opis:
    The cucurbitane-type triterpenoid glycosides, mogrosides, are the main active components of Siraitia grosvenorii fruit. Squalene and cucurbitadienol are among the intermediates of the biosynthetic pathway for the formation of cucurbitane-type triterpenoid backbones of mogrosides. It is recognized that the exogenous application of methyl jasmonate (MeJA) increases the accumulation of secondary metabolites in various plant species. Here, the effect of MeJA (50, 200, and 500 μM) on the accumulation of squalene and cucurbitadienol in the fruits of S. grosvenorii at 10, 20, and 30 days after flowering (DAF) was tested for the first time. Since mogroside II E is the main cucurbitane-type triterpenoid present at this time, its concentration was also determined. The results show that MeJA can indeed promote squalene and cucurbitadienol accumulation, the application of 500 μM MeJA at 30 DAF being optimal. The concentration of squalene and cucurbitadienol increased up to 0.43 and 4.71 μg/g dry weight (DW), respectively, both of which were 1.2-fold greater than that of the control. The content of mogroside II E increased by 15% over the untreated group. We subsequently analyzed the expression of key genes involved in the mogroside biosynthetic pathway, including the 3-hydroxy-3-methylglutaryl-coenzyme A reductase gene (SgHMGR), squalene synthetase gene (SgSQS), cucurbitadienol synthase gene (SgCS), and cytochrome P450 (SgCYP450) with quantitative real-time PCR. The results showed that transcriptional levels of these genes were upregulated following the treatment described above. Additionally, their responses in the presence of MeJA was related to the concentration and timing of MeJA treatment.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2016, 85, 3
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-5 z 5

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