- Tytuł:
- Extracellular matrix surface network is associated with non-morphogenic calli of Helianthus tuberosus cv. Albik produced from various explants
- Autorzy:
-
Pilarska, M.
Popielarska-Konieczna, M.
Slesak, H.
Kozieradzka-Kiszkurno, M.
Goralski, G.
Konieczny, R.
Bohdanowicz, J.
Kuta, E. - Powiązania:
- https://bibliotekanauki.pl/articles/58028.pdf
- Data publikacji:
- 2014
- Wydawca:
- Polskie Towarzystwo Botaniczne
- Tematy:
-
callogenesis
extracellular matrix
surface network
in vitro culture
non-morphogenic callus
Helianthus tuberosus
Albik cultivar
explant
transmission electron microscopy - Opis:
- Helianthus tuberosus is economically important species. To improve characters of this energetic plant via genetic modification, production of callus tissue and plant regeneration are the first steps. A new, potentially energetic cultivar Albik was used in this study to test callus induction and regeneration. Callus was produced on leaves, petioles, apical meristems and stems from field-harvested plants but was totally non-morphogenic. Its induction started in the cortex and vascular bundles as confirmed by histological analysis. The surface of heterogeneous callus was partially covered with a membranous extracellular matrix surface network visible in scanning and transmission electron microscopies. The results clearly indicate that: (i) the morphogenic capacity of callus in topinambur is genotype dependent, (ii) cv. Albik of H. tuberosus proved recalcitrant in in vitro regeneration, and (iii) extracellular matrix surface network is not a morphogenic marker in this cultivar.
- Źródło:
-
Acta Societatis Botanicorum Poloniae; 2014, 83, 1
0001-6977
2083-9480 - Pojawia się w:
- Acta Societatis Botanicorum Poloniae
- Dostawca treści:
- Biblioteka Nauki
Artykuł