Temat: site-specific - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: A rapid and simple method for detection of type II restriction endonucleases in cells of bacteria with high activity of nonspecific nucleases
Autorzy:: Podgórska, Beata
Kujawska, Grażyna
Skurzewski, Michał
Batsko, Olesya
Kaczorowski, Tadeusz
Powiązania:: https://bibliotekanauki.pl/articles/1039682.pdf
Data publikacji:: 2012
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: site-specific endonuclease purification
type II restriction endonuclease
bacteria screening
Opis:: In this work we describe a novel, rapid and simple microscale procedure for identification of restriction endonuclease activity in bacteria lysates, which contain high levels of non-specific DNA nucleases.
Źródło:: Acta Biochimica Polonica; 2012, 59, 4; 669-672
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Two types of non-homologous RNA recombination in brome mosaic virus
Autorzy:: Alejska, Magdalena
Malinowska, Nelli
Urbanowicz, Anna
Figlerowicz, Marek
Powiązania:: https://bibliotekanauki.pl/articles/1041326.pdf
Data publikacji:: 2005
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: non-homologous RNA recombination
site-specific RNA recombination
heteroduplex-mediated RNA recombination
brome mosaic virus
Opis:: Non-homologous RNA recombination is a process enabling the exchange of genetic material between various (related or unrelated) RNA-based viruses. Despite extensive investigations its molecular mechanism remains unclear. Studies on genetic recombination in brome mosaic virus (BMV) have shown that local hybridization between genomic RNAs induces frequent non-homologous crossovers. A detailed analysis of recombinant structures suggested that local complementary regions might be involved in two types of non-homologous recombination in BMV: site-specific and heteroduplex-mediated. To verify the above hypothesis and better recognize the mechanism of the phenomenon studied we have tested how the putative types of recombination are affected by a specific mutation in the BMV polymerase gene or by changes in RNA structure. The experiments undertaken revealed substantial differences between site-specific and heteroduplex-mediated recombination, indicating that they occur according to different mechanisms. The former can be classified as homology-assisted, and the latter as homology-independent. In addition to local RNA/RNA hybridization, short regions of homology are required for site-specific crossovers to occur. They are most efficiently mediated if one homologous sequence is located at the beginning of and the second just before a double-stranded region. At present it is difficult to state what is the mechanism of heteroduplex-mediated recombination. Earlier it was postulated that strong RNA/RNA interaction enforces template switching by the viral replicase. There are, however, several observations questioning this model and indicating that some other factors, which are still unknown, may influence heteroduplex-mediated crossovers.
Źródło:: Acta Biochimica Polonica; 2005, 52, 4; 833-844
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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