- Tytuł:
- Quantification of thioredoxin mRNA expression in the rat hippocampus by real-time PCR following oxidative stress.
- Autorzy:
- Yalcin, Ayfer
- Powiązania:
- https://bibliotekanauki.pl/articles/1041524.pdf
- Data publikacji:
- 2004
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
kainic acid
real-time polymerase chain reaction
oxidative stress
thioredoxin - Opis:
- Thioredoxin (Trx) is a multifunctional protein with a redox-active disulfide/dithiol in the active site. Thioredoxin, with its redox-regulating and reactive oxygen species (ROS) scavenging activities, plays several important biologic roles both in intracellular and extracellular compartments. The purpose of this report was to quantify the relative expression of Trx in rat hippocampus following an oxidative stress-involving treatment such as kainic acid (KA) using real-time PCR and the 2-ΔΔCT method. The relative changes in expression of Trx mRNA in KA-treated and control animals were significantly different as 2.02 ± 0.77 and 1.0 ± 0.26, respectively ( P <0.05). Minimum and maximum n-fold changes in Trx expression in KA-treated and control animals were determined as (1.4-5.2) and (0.8-1.3), respectively. Thus, real-time PCR and the 2-ΔΔCT method for data analysis from real-time PCR were found to be an accurate and sensitive method for quantifying Trx mRNA levels.
- Źródło:
-
Acta Biochimica Polonica; 2004, 51, 4; 1059-1065
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł