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Wyszukujesz frazę "Endocytosis" wg kryterium: Temat

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    Wyświetlanie 1-3 z 3
    Tytuł:
    Saccharomyces cerevisiae - a model organism for the studies on vacuolar transport.
    Autorzy:
    Kucharczyk, Róża
    Rytka, Joanna
    Powiązania:
    https://bibliotekanauki.pl/articles/1044044.pdf
    Data publikacji:
    2001
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    vacuole
    vesicles
    intracellular transport
    phagocytosis
    endocytosis
    yeast
    Opis:
    The role of the yeast vacuole, a functional analogue of the mammalian lysosome, in the turnover of proteins and organelles has been well documented. This review provides an overview of the current knowledge of vesicle mediated vacuolar transport in the yeast Saccharomyces cerevisiae cells. Due to the conservation of the molecular transport machinery S. cerevisiae has become an important model system of vacuolar trafficking because of the facile application of genetics, molecular biology and biochemistry.
    Źródło:
    Acta Biochimica Polonica; 2001, 48, 4; 1025-1042
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Mechanosensitivity of cell membrane may govern creep-strain recovery, osmotic expansion and lysis
    Autorzy:
    Pawłowski, Piotr
    Powiązania:
    https://bibliotekanauki.pl/articles/1040543.pdf
    Data publikacji:
    2009
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    mechanosensitivity
    caveolae
    exocytosis
    surface tension
    endocytosis
    cell membrane
    Opis:
    A simple theoretical model considering cell membrane mechanosensitivity can accurately describe published experimental data on membrane area creeping and recovery, and on osmotic expansion and rupture. The model to data fit reveals real values of membrane tension and elasticity modulus, and the parameters describing membrane organization and kinetics of mechanosensitive membrane traffic, including small solute transport, water permeability, endocytosis, exocytosis, and caveolae formation. This estimation allows for separation and quantitative analysis of the participation of different processes constituting the response of plasmalemma to short time-scale membrane load. The predicted properties of the model were verified for membrane stretching at different osmotic pressures. Finally, a simple hypothesis concerning stressed cell membrane breakdown is postulated.
    Źródło:
    Acta Biochimica Polonica; 2009, 56, 3; 471-480
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Distinct role of clathrin-mediated endocytosis in the functional uptake of cholera toxin
    Autorzy:
    Broeck, Davy
    Lagrou, Albert
    De Wolf, Marc
    Powiązania:
    https://bibliotekanauki.pl/articles/1040864.pdf
    Data publikacji:
    2007
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    clathrin-mediated endocytosis
    internalization
    chlorpromazine
    Cholera toxin
    siRNA
    Opis:
    The involvement of the clathrin-mediated endocytic internalization route in the uptake of cholera toxin (CT) was investigated using different cell lines, including the human intestinal Caco-2 and T84 cell lines, green monkey Vero cells, SH-SY5Y neuroblastoma cells and Madin-Darby canine kidney cells. Suppression of the clathrin-mediated endocytic pathway by classical biochemical procedures, like intracellular acidification and potassium depletion, inhibited cholera toxin uptake by up to about 50% as well as its ability to raise intracellular levels of cAMP. Also prior exposure of these cell types to the cationic amphiphilic drug chlorpromazine reduced the functional uptake of cholera toxin, even to a greater extent. These effects were dose- and cell type-dependent, suggesting an involvement of clathrin-mediated endocytosis in the functional uptake of cholera toxin. For a more straightforward approach to study the role of the clathrin-mediated uptake in the internalization of cholera toxin, a Caco-2eps- cell line was exploited. These Caco-2eps- cells constitutively suppress the expression of epsin, an essential accessory protein of clathrin-mediated endocytosis, thereby selectively blocking this internalization route. CT uptake was found to be reduced by over 60% in Caco-2eps- paralleled by a diminished ability of CT to raise the level of cAMP. The data presented suggest that the clathrin-mediated uptake route fulfils an important role in the functional internalization of cholera toxin in several cell types.
    Źródło:
    Acta Biochimica Polonica; 2007, 54, 4; 757-767
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-3 z 3

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