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    Wyświetlanie 1-6 z 6
    Tytuł:
    Screening of environmental samples for bacteria producing 1,3-propanediol from glycerol
    Autorzy:
    Dąbrowski, Sławomir
    Zabłotna, Ewa
    Pietrewicz-Kubicz, Dorota
    Długołęcka, Anna
    Powiązania:
    https://bibliotekanauki.pl/articles/1039709.pdf
    Data publikacji:
    2012
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    anaerobic fermentation
    Clostridium
    screening
    biodiesel
    glycerol
    1,3-propanediol
    Opis:
    Twenty nine environmental samples were screened for the presence of anaerobic microorganisms fermenting glycerol with 1,3-propanediol as a final product. Seven samples were then selected for the next step of our research and eight bacteria strains were cultured anaerobically. Seven of them produced 1,3-propanediol with a yield of 0.47-0.58. Six of the the isolated microorganisms were then classified as Clostridium butyricum (four strains), C. lituseburense (one strain), and C. sartagoforme (one strain). We suggest that of all these strains C. butyricum 2CR371.5 is the best 1,3-propanediol producer as producing no lactate as a by-product and growing well on a glycerol-containing medium.
    Źródło:
    Acta Biochimica Polonica; 2012, 59, 3; 353-356
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Hypothetical glycerol pathways of newly isolated strains capable of 1,3-propanediol production
    Autorzy:
    Leja, Katarzyna
    Samul, Dorota
    Drożdżyńska, Agnieszka
    Myszka, Kamila
    Juzwa, Wojciech
    Pawlicka, Joanna
    Czaczyk, Katarzyna
    Powiązania:
    https://bibliotekanauki.pl/articles/1039209.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Clostridium bifermentans
    Clostridium butyricum
    Hafnia alvei
    1,3-propanediol
    Opis:
    Study presented here demonstrates the ability of three newly isolated strains, obtained from environmental probes (manure, bottom sediment, and food waste) and identified as Clostridium bifermentans, Clostridium butyricum, and Hafnia alvei, to synthesize 1,3-propanediol (1,3-PD), organic acids (such as lactic, acetic, fumaric, succinic, and butyric acids), and ethanol from glycerol. The production of 1,3-PD as well as the glycerol pathways in C. bifermentans and H. alvei cells have not been investigated and described yet by others. Moreover, there is no data in the available literature on the products of glycerol utilization by H. alvei and there is only some incoherent data (mainly from the first half of the twentieth century) about the ability of C. bifermentans to carry out glycerol degradation. Additionally, this study presents complete hypothetical glycerol pathways and the basic fermentation kinetic parameters (such as yield and productivity) for both strains as well as for the newly isolated C. butyricum strain.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 4; 759-763
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Biotechnological conversion of glycerol from biofuels to 1,3-propanediol using Escherichia coli
    Autorzy:
    Przystałowska, Hanna
    Lipiński, Daniel
    Słomski, Ryszard
    Powiązania:
    https://bibliotekanauki.pl/articles/1039124.pdf
    Data publikacji:
    2015
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    bioconversion
    biodiesel
    carbon source
    Escherichia coli
    glycerol
    1,3-propanediol
    Opis:
    In the face of shortage of fossil fuel supplies and climate warming triggered by excessive carbon dioxide emission, alternative resources for chemical industry have gained considerable attention. Renewable resources and their derivatives are of particular interest. Glycerol, which constitutes one of the by-products during biodiesel production, is such a substrate. Thus, generated excess glycerol may become an environmental problem, since it cannot be disposed of in the environment. The most promising products obtained from glycerol are polyols, including 1,3-propanediol, an important substrate in the production of synthetic materials, e.g. polyurethanes, unsaturated polyesters, and epoxy resins. Glycerol can be used as a carbon and energy source for microbial growth in industrial microbiology to produce 1,3-propanediol. This paper is a review of metabolic pathways of native producers and E. coli with the acquired ability to produce the diol via genetic manipulations. Culture conditions during 1,3-PDO production and genetic modifications of E. coli used in order to increase efficiency of glycerol bioconversion are also described in this paper.
    Źródło:
    Acta Biochimica Polonica; 2015, 62, 1; 23-34
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    1,3-propanediol production by Escherichia coli expressing genes of dha operon from Clostridium butyricum 2CR371.5
    Autorzy:
    Dąbrowski, Sławomir
    Pietrewicz-Kubicz, Dorota
    Zabłotna, Ewa
    Długołęcka, Anna
    Powiązania:
    https://bibliotekanauki.pl/articles/1039710.pdf
    Data publikacji:
    2012
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    anaerobic fermentation
    Clostridium butyricum
    Escherichia coli
    biodiesel
    dha operon
    glycerol
    1,3-propanediol
    Opis:
    1,3-propanediol is used as a monomer in the production of some polymers e.g. polytrimethylene terephthalate used in the production of carpets and textile fibers and in the thermoplastics engineering. However, the traditional chemical synthesis is expensive, generates some toxic intermediates and requires a reduction step under high hydrogen pressure. Biological production of 1,3-propanediol could be an attractive alternative to the traditional chemical methods. Moreover, crude glycerol which is a by-product of biodiesel production, can be used. We constructed a recombinant Escherichia coli strain producing 1,3-propanediol from glycerol by introducing genes of the dha operon from Clostridium butyricum 2CR371.5, a strain from our collection of environmental samples and strains. The E. coli strain produced 3.7 g of 1,3-propanediol per one litre of culture with the yield of 0.3 g per 1 g of glycerol consumed.
    Źródło:
    Acta Biochimica Polonica; 2012, 59, 3; 357-361
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Group II intron-mediated deletion of lactate dehydrogenase gene in an isolated 1,3-propanediol producer Hafnia alvei AD27
    Autorzy:
    Celińska, Ewelina
    Drożdżyńska, Agnieszka
    Wita, Agnieszka
    Juzwa, Wojciech
    Białas, Wojciech
    Czaczyk, Katarzyna
    Grajek, Włodzimierz
    Powiązania:
    https://bibliotekanauki.pl/articles/1038697.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    1,3-propanediol
    gene knock-out
    metabolic engineering
    Enterobacteriaceae
    group II intron
    lactate dehydrogenase
    Opis:
    Our previous studies showed that glycerol fermentation by Hafnia alvei AD27 strain was accompanied by formation of high quantities of lactate. The ultimate aim of this work was the elimination of excessive lactate production in the 1,3-propanediol producer cultures. Group II intron-mediated deletion of ldh (lactate dehydrogenase) gene in an environmental isolate of H. alvei AD27 strain was conducted. The effect of the Δldh genotype in H. alvei AD27 strain varied depending on the culture medium applied. Under lower initial glycerol concentration (20 gL-1), lactate and 1,3-propanediol production was fully abolished, and the main carbon flux was directed to ethanol synthesis. On the other hand, at higher initial glycerol concentrations (40 gL-1), 1,3-propanediol and lactate production was recovered in the recombinant strain. The final titers of 1,3-propanediol and ethanol were similar for the recombinant and the WT strains, while the Δldh genotype displayed significantly decreased lactate titer. The by-products profile was altered upon ldh gene deletion, while glycerol utilization and biomass accumulation remained unaltered. As indicated by flow-cytometry analyses, the internal pH was not different for the WT and the recombinant Δldh strains over the culture duration, however, the WT strain was characterized by higher redox potential.
    Źródło:
    Acta Biochimica Polonica; 2017, 64, 1; 123-133
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    1,3-Propanediol production by Escherichia coli using genes from Citrobacter freundii atcc 8090
    Autorzy:
    Przystałowska, Hanna
    Zeyland, Joanna
    Kośmider, Alicja
    Szalata, Marlena
    Słomski, Ryszard
    Lipiński, Daniel
    Powiązania:
    https://bibliotekanauki.pl/articles/1039012.pdf
    Data publikacji:
    2015
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    bio-based 1,3-propanediol
    genetically modified bacteria
    glycerol utilization
    renewable resources
    Citrobacter freundii
    Escherichia coli
    Opis:
    Compared with chemical synthesis, fermentation has the advantage of mass production at low cost, and has been used in the production of various industrial chemicals. As a valuable organic compound, 1,3-propanediol (1,3-PDO) has numerous applications in the production of polymers, lubricants, cosmetics and medicines. Here, conversion of glycerol (a renewable substrate and waste from biodiesel production) to 1,3-PDO by E. coli bacterial strain carrying altered glycerol metabolic pathway was investigated. Two gene constructs containing the 1,3-PDO operon from Citrobacter freundii (pCF1 and pCF2) were used to transform the bacteria. The pCF1 gene expression construct contained dhaBCE genes encoding the three subunits of glycerol dehydratase, dhaF encoding the large subunit of the glycerol dehydratase reactivation factor and dhaG encoding the small subunit of the glycerol dehydratase reactivating factor. The pCF2 gene expression construct contained the dhaT gene encoding the 1,3-propanediol dehydrogenase. Expression of the genes cloned in the above constructs was under regulation of the T7lac promoter. RT-PCR, SDS-PAGE analyses and functional tests confirmed that 1,3-PDO synthesis pathway genes were expressed at the RNA and protein levels, and worked flawlessly in the heterologous host. In a batch flask culture, in a short time applied just to identify the 1,3-PDO in a preliminary study, the recombinant E. coli bacteria produced 1.53 g/L of 1,3-PDO, using 21.2 g/L of glycerol in 72 h. In the Sartorius Biostat B Plus reactor, they produced 11.7 g/L of 1,3-PDO using 24.2 g/L of glycerol, attaining an efficiency of 0.58 [mol1,3-PDO/molglycerol].
    Źródło:
    Acta Biochimica Polonica; 2015, 62, 3; 589-597
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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