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    Wyświetlanie 1-8 z 8
    Tytuł:
    S100A4 promotes invasion and angiogenesis in breast cancer MDA-MB-231 cells by upregulating matrix metalloproteinase-13
    Autorzy:
    Wang, Lin
    Wang, Xingang
    Liang, Yu
    Diao, Xinying
    Chen, Qingfeng
    Powiązania:
    https://bibliotekanauki.pl/articles/1039658.pdf
    Data publikacji:
    2012
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    S100A4
    MMP-13
    metastasis
    breast cancer
    Opis:
    S100A4 is a member of the S100 family of calcium-binding proteins that is directly involved in tumor metastasis. In the present study, we examined the potential role of S100A4 in metastasis in breast cancer and its relation with matrix metalloproteinase-13 (MMP-13). Analysis of 100 breast cancer specimens including 50 with and 50 without lymph node metastasis showed a significant upregulation of S100A4 and MMP-13 expression in metastatic breast cancer tissues. Positive immunoreactivity for S100A4 was associated with MMP-13 expression. Overexpression of S100A4 in the MDA-MB-231 breast cancer cell line upregulated MMP13 expression leading to increased cell migration and angiogenesis. SiRNA-mediated silencing of S100A4 downregulated MMP13 expression and suppressed cell migration and angiogenesis. Moreover, neutralization of MMP-13 activity with a specific antibody blocked cell migration and angiogenesis in MDA-MB-231/S100A4 cells. In vivo siRNA silencing of S100A4 significantly inhibited lung metastasis in transgenic mice. The present results suggest that the S100A4 gene may control the invasive potential of human breast cancer cells by modulating MMP-13 levels, thus regulating metastasis and angiogenesis in breast tumors. S100A4 could therefore be of value as a biomarker of breast cancer progression and a novel therapeutic target for human breast cancer treatment.
    Źródło:
    Acta Biochimica Polonica; 2012, 59, 4; 593-598
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Comparative proteomic analysis of Bombyx mori hemolymph and fat body after calorie restriction
    Autorzy:
    Chen, Huiqing
    Li, Yijia
    Chen, Keping
    Yao, Qin
    Li, Guohui
    Wang, Lin
    Powiązania:
    https://bibliotekanauki.pl/articles/1040312.pdf
    Data publikacji:
    2010
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    calorie restriction
    Bombyx mori
    two-dimensional gel electrophoresis
    proteomic analysis
    MALDI-TOF/TOF MS
    Opis:
    Calorie restriction (CR) is known to extend life span from yeast to mammals. To gain an insight into the effects of CR on growth and development of the silkworm Bombyx mori at protein level, we employed comparative proteomic approach to investigate proteomic differences of hemolymph and fat body of the silkworm larvae subjected to CR. Thirty-nine differentially expressed proteins were identified by MALDI TOF/TOF MS. Among them, 19 were from the hemolymph and 20 from the fat body. The hemolymph of the CR group contained two down-regulated and 17 up-regulated proteins, whereas the fat body contained 15 down-regulated and five up-regulated ones. These proteins belonged to those functioning in immune system, in signal transduction and apoptosis, in regulation of growth and development, and in energy metabolism. Our results suggest that CR can alter the expression of proteins related to the above four aspects, implying that these proteins may regulate life span of the silkworm through CR.
    Źródło:
    Acta Biochimica Polonica; 2010, 57, 4; 505-511
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Molecular cloning, expression and characterization of Bmserpin-2 gene from Bombyx mori
    Autorzy:
    Pan, Ye
    Xia, Hengchuan
    Lü, Peng
    Chen, KePing
    Yao, Qin
    Chen, Huiqin
    Gao, Lu
    He, Yuanqing
    Wang, Lin
    Powiązania:
    https://bibliotekanauki.pl/articles/1040486.pdf
    Data publikacji:
    2009
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    serpin-2
    Bombyx mori
    bioinformatics
    subcellular location
    qPCR
    Opis:
    Serpins are a broadly distributed family of protease inhibitors. In this study, the gene encoding Bombyx mori serpin-2 (Bmserpin-2) was cloned and expressed in E. coli. The Bmserpin-2 cDNA contains a 1125 bp open reading frame (ORF). The deduced protein has 374 amino-acid residues, contains a conserved SERPIN domain and shares extensive homology with other invertebrate serpins. RT-PCR analysis showed that Bmserpin-2 was expressed in all developmental stages of B. mori larvae and various larval tissues. Subcellular localization analysis indicated that Bmserpin-2 protein was located in the cytoplasm. Interestingly, real-time quantitative PCR revealed that the expression of Bmserpin-2 in the midgut of susceptible B. mori strain 306 significantly increased at 72 hours post inoculation (hpi) when infected with BmNPV. However, there was no significant increase of the Bmserpin-2 expression in resistant strain NB infected with BmNPV. Thus, our data indicates that Bmserpin-2 may be involved in B. mori antiviral response.
    Źródło:
    Acta Biochimica Polonica; 2009, 56, 4; 671-677
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Newly identified transcripts of UL4 and UL5 genes of human cytomegalovirus
    Autorzy:
    Gao, Shuang
    Ruan, Shan
    Ma, Yanping
    Li, Mali
    Wang, Lin
    Zheng, Bo
    Qi, Ying
    Sun, Zhengrong
    Huang, Yujing
    Ruan, Qiang
    Powiązania:
    https://bibliotekanauki.pl/articles/1039141.pdf
    Data publikacji:
    2015
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    HCMV
    UL4
    UL5
    transcript
    Opis:
    Human cytomegalovirus (HCMV) UL4 and UL5 genes are two members of the RL11 gene family. In an earlier study, three UL4 transcripts of about 1.7, 1.5 and 1.4 kb were found in early and late classes after infection by the Towne strain by nuclease protection and primer extension analyses. In the present study, two UL4 transcripts (1.5 and 1.7 kb) were found by cDNA library screening, Northern blot, 3' and 5' RACE analyses to appear initially in the immediate early phase and one UL4 transcript (1.4 kb) in the late phase in a low-passage clinical isolate. Furthermore, two novel low-abundance UL5 transcripts with the same 3' terminus as the identified UL4 transcripts in the UL4-UL5 gene region were found in late class RNAs.
    Źródło:
    Acta Biochimica Polonica; 2015, 62, 1; 97-101
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of deletion mutation on the recombination activity of Cre recombinase
    Autorzy:
    Rongrong, Liu
    Lixia, Wang
    Zhongping, Lin
    Powiązania:
    https://bibliotekanauki.pl/articles/1041446.pdf
    Data publikacji:
    2005
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Cre recombinase
    deletion mutation
    in vitro recombination assay
    Opis:
    Cre recombinase from bacteriophage P1 is widely used in both in vitro and in vivo DNA manipulations. Based on a structural and functional analysis, three deleted cre mutants were constructed and expressed in Escherichia coli. Mutated recombinases were purified and their recombination activities were determined in vitro. Our results revealed that the mutant with amino-terminal deletion retains the recombination activity as high as wild type Cre; however, the carboxy-terminal deletion and the middle region deletion both lead to a complete loss of the recombinase function.
    Źródło:
    Acta Biochimica Polonica; 2005, 52, 2; 541-544
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Sp100 interacts with phage ΦC31 integrase to inhibit its recombination activity
    Autorzy:
    Lin, Yun
    Li, Zhi-Hui
    Wang, Jing-Jing
    Xu, Gua-Lan
    Shen, Qi
    Tian, Lin
    Xue, Jin-Lun
    Chen, Jin-Zhong
    Powiązania:
    https://bibliotekanauki.pl/articles/1039951.pdf
    Data publikacji:
    2011
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Sp100
    ΦC31 integrase
    recombination
    Opis:
    Phage ΦC31 integrase is a potential vector for the insertion of therapeutic genes into specific sites in the human genome. To understand the mechanism involved in ΦC31 integrase-mediated recombination, it is important to understand the interaction between the integrase and cellular proteins. Using a yeast two-hybrid system with pLexA-ΦC31 integrase as bait, we screened a pB42AD human fetal brain cDNA library for potential interacting cellular proteins. From the 106 independent clones that were screened, 11 potential interacting clones were isolated, of which one encoded C-terminal fragment of Sp100. The interaction between Sp100 and ΦC31 integrase was further confirmed by yeast mating and co-immunoprecipitation assays. The hybridization between a ΦC31 integrase peptide array and an HEK293 cell extract revealed that residues 81RILN84 in the N-terminus of ΦC31 integrase are responsible for the interaction with Sp100. Knocking down endogenous Sp100 with Sp100-specific siRNA increased ΦC31 integrase-mediated recombination but did not impact reporter gene expression. Therefore, endogenous Sp100 may interact with ΦC31 integrase and inhibit the efficiency of ΦC31 integrase-mediated recombination.
    Źródło:
    Acta Biochimica Polonica; 2011, 58, 1; 67-73
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Amino acid substitutions of His296 alter the catalytic properties of Zymomonas mobilis 10232 levansucrase
    Autorzy:
    Li, Shu
    Chen, Ming
    Li, Gang
    Yan, Yong
    Yu, Hai
    Zhan, Yu
    Peng, Zi
    Wang, Jin
    Lin, Min
    Powiązania:
    https://bibliotekanauki.pl/articles/1040841.pdf
    Data publikacji:
    2008
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Zymomonas mobilis
    His296
    levansucrase
    levan
    transfructosylation activity
    hydrolysis activity
    Opis:
    His296 of Zymomonas mobilis levansucrase (EC 2.4.1.10) is crucial for the catalysis of the transfructosylation reaction. The three-dimensional structures of levansucrases revealed the His296 is involved in the substrate recognition and binding. In this study, nine mutants were created by site-directed mutagenesis, in which His296 was substituted with amino acids of different polarity, charge and length. The substitutions of His296 with Arg or Trp retained partial hydrolysis and transfructosylation activities. The positively charged Lys substitution resulted in a 2.5-fold increase of sucrose hydrolysis. Substitutions with short (Cys or Ser), negatively charged (Glu) or polar (Tyr) amino acids virtually abolished both the activities. Analysis of transfructosylation products indicated that the mutants synthesized different oligosaccharides, suggesting that amino acid substitutions of His296 strongly affected both the enzyme activity and transfructosylation products.
    Źródło:
    Acta Biochimica Polonica; 2008, 55, 1; 201-206
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    cDNA cloning, gene organization and expression analysis of human peptidylarginine deiminase type VI.
    Autorzy:
    Zhang, Jiayi
    Dai, Jianliang
    Zhao, Enpeng
    Lin, Yun
    Zeng, Li
    Chen, Jinzhong
    Zheng, Huari
    Wang, Yu
    Li, Xin
    Ying, Kang
    Xie, Yi
    Mao, YuMin
    Powiązania:
    https://bibliotekanauki.pl/articles/1041522.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    ovary
    ePAD
    peptidylarginine deiminase
    hPADVI
    Opis:
    Peptidylarginine deiminase (PAD) catalyzes the post-translational modification of protein through the conversion of arginine to citrulline in the presence of calcium ions. Human, similar to rodents, has four isoforms of PAD (type I, II, III and IV/V), each of which is distinct in substrate specificity and tissue specific expression. In our large-scale sequencing project, we identified a new human PAD cDNA from a human fetal brain cDNA library. The putative protein encoded by this cDNA is designated hPADVI. Expression analysis of hPADVI showed that it is mainly expressed in adult human ovary and peripheral blood leukocytes. We conclude that hPADVI may be orthologous to mouse ePAD, basing on sequence comparison, chromosome localization and exon-intron structure analysis. PAD-mediated deimination of epithelial cell keratin resulting in cytoskeletal remodeling suggests a possible role for hPADVI in cytoskeletal reorganization in the egg and in early embryo development. This study describes a new important member of the human PAD family.
    Źródło:
    Acta Biochimica Polonica; 2004, 51, 4; 1051-1058
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-8 z 8

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