Autor: Grabowska, Iwona - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Electrochemical biosensors for detection of avian influenza virus - current status and future trends
Autorzy:: Grabowska, Iwona
Malecka, Kamila
Jarocka, Urszula
Radecki, Jerzy
Radecka, Hanna
Powiązania:: https://bibliotekanauki.pl/articles/1039249.pdf
Data publikacji:: 2014
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: electrochemical biosensors
genosensors
immunosensors
electrochemical detection
avian influenza virus
Opis:: Electrochemical biosensors have emerged as reliable analytical devices suitable for pathogen detection. Low cost, small sample requirement and possibility of miniaturization justifies their increasing development. Thus, we report in this review on the state of the art of avian influenza virus detection with genosensors and immunosensors working by an electrochemical mode. Their working principles focusing on the physical properties of the transducer, the immobilization chemistry, as well as new trends including incorporation of nanoparticles will be presented. Then, we critically review the detection of avian influenza virus in the complex matrices that use electrochemical biosensors and compare them with traditionally applied methods such as ELISA or Western blot.
Źródło:: Acta Biochimica Polonica; 2014, 61, 3; 471-478
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Barley primary microRNA expression pattern is affected by soil water availability
Autorzy:: Swida-Barteczka, Aleksandra
Kruszka, Katarzyna
Grabowska, Aleksandra
Pacak, Andrzej
Jarmolowski, Artur
Kurowska, Marzena
Szarejko, Iwona
Szweykowska-Kulinska, Zofia
Powiązania:: https://bibliotekanauki.pl/articles/1038746.pdf
Data publikacji:: 2016
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: pri-miRNA
miRNA
drought
rehydration
barley genotypes
Opis:: MicroRNAs are short molecules of 21-24 nt in length. They are present in all eukaryotic organisms and regulate gene expression by guiding posttranscriptional silencing of mRNAs. In plants, they are key players in signal transduction, growth and development, and in response to abiotic and biotic stresses. Barley (Hordeum vulgare) is an economically important monocotyledonous crop plant. Drought is the world's main cause of loss in cereal production. We have constructed a high-throughput Real-Time RT-qPCR platform for parallel determination of 159 barley primary microRNAs' levels. The platform was tested for two drought-and-rehydration-treated barley genotypes (Rolap and Sebastian). We have determined changes in the expression of primary microRNAs responding to mild drought, severe drought, and rehydration. Based on the results obtained, we conclude that alteration in the primary microRNA expression is relative to the stress's intensity. Mild drought and rehydration mostly decrease the pri-miRNA levels in both of the tested genotypes. Severe drought mainly induces the primary microRNA expression. The main difference between the genotypes tested was a much-stronger induction of pri-miRNAs in Rolap encountering severe drought. The primary microRNAs respond dynamically to mild drought, severe drought, and rehydration treatments. We propose that some of the individual pri-miRNAs could be used as drought stress or rehydration markers. The usage of the platform in biotechnology is also postulated.
Źródło:: Acta Biochimica Polonica; 2016, 63, 4; 817-824
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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